FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1124375
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACGGAACCACTCAGGCTGCTGTCATAGGA36040.3205336297943302No Hit
GGTTATGGTGTCCACTGGTACCAGCAGGTTCCAGGAACCGTCCCCAAACTCCTCATCTATGGTAACAACAATCGG35800.3183991106170095No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACGGAACCACTCAGG34090.3031906614785992No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGGTGTCCACTGGTACCAGCAGGTTCCAGGAACCGT31920.2838910505836576No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31020.2758866036687048No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCTTCAGCCTGGAGCCCAG27550.24502501389660922No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26080.23195108393551972No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24960.2219899944413563No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC23860.21220678154530292No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22960.2042023346303502No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22650.20144524735964423No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC22080.1963757643135075No Hit
CCATAACCTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGACGGTGACCCACTGCCCTGGGGCCCCAGACA21300.1894385769872151No Hit
GTCCTATGACAGCAGCCTGAGTGGTTCCGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG21290.1893496386881601No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20660.18374652584769316No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC20620.18339077265147305No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG20140.17912173429683156No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19120.17005002779321846No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG18430.16391328515842135No Hit
GGCCATCACTGGGCTCCAGGCTGAAGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTTC18210.16195664257921066No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16890.15021678710394665No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16710.1486158977209561No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT16540.14710394663702056No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16510.14683713173985546No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16010.14239021678710395No Hit
GTGCAGGAGACGGTGACCCACTGCCCTGGGGCCCCAGACACTGAGGGCGG15830.14078932740411337No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15690.139544191217343No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15620.13892162312395775No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC15590.13865480822679266No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15480.13767648693718731No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15200.1351862145636465No Hit
GTGTCTGGGGCCCCAGGGCAGTGGGTCACCGTCTCCTGCACTGGGAGCAG15090.13420789327404115No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14920.1326959421901056No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACGGAACCACTCAGGC14880.1323401889938855No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT14820.1318065591995553No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14780.13145080600333517No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT14630.13011673151750974No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14550.1294052251250695No Hit
GATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTTCCGTGTTCGG14460.1286047804335742No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT14040.12486937187326294No Hit
TCACTGGGCTCCAGGCTGAAGATGAGGCTGATTATTACTGCCAGTCCTAT13220.11757643135075042No Hit
GTAATAATCAGCCTCATCTTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG12960.11526403557531961No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12950.11517509727626458No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12930.11499722067815452No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12710.11304057809894386No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12570.11179544191217343No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA12560.1117065036131184No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12400.1102834908282379No Hit
GTTTGGGGACGGTTCCTGGAACCTGCTGGTACCAGTGGACACCATAACCT12390.11019455252918287No Hit
GGGCAGTGGGTCACCGTCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGGTGTCCACTGGTACCAG12300.10939410783768762No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12260.1090383546414675No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC11810.10503613118399109No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAAGATG11640.1035241801000556No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11620.10334630350194553No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACGGAAC11610.1032573652028905No Hit
GTTCCTGGAACCTGCTGGTACCAGTGGACACCATAACCTGCCCCGATGTT11600.10316842690383547No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11520.10245692051139522No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11420.10156753752084491No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11400.10138966092273485No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11340.10085603112840466No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11340.10085603112840466No Hit
GTATCAACGCAGAGTACGGGAGGCTCTGCTTCAGCTGTGGGCACAAGAGG11280.10032240133407448No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC11270.10023346303501945No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)