FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1124375
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTATGGTGTCCACTGGTACCAGCAGGTTCCAGGAACCGTCCCCAAACTCCTCATCTATGGTAACAACAATCGG34640.3080822679266259No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGGTGTCCACTGGTACCAGCAGGTTCCAGGAACCGT30800.27392996108949413No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACGGAACCACTCAGGCTGCTGTCATAGGA30140.2680600333518622No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30120.2678821567537521No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGTGGGTCACCGTCTCCTG27550.24502501389660922No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACGGAACCACTCAGG25660.22821567537520845No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25170.22385769872151196No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24730.21994441356309058No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCTTCAGCCTGGAGCCCAG23510.2090939410783769No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22030.19593107281823235No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGTGGGTCAC21920.194952751528627No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21740.19335186214563646No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21120.18783768760422456No Hit
GTCCTATGACAGCAGCCTGAGTGGTTCCGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG20860.1855252918287938No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC20430.18170094496942746No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19630.174585881045025No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT19610.17440800444691495No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG19260.17129516397998887No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18410.1637354085603113No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17670.157153974430239No Hit
GGCCATCACTGGGCTCCAGGCTGAAGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTTC17250.15341856586992775No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16830.14968315730961645No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGTGGGTCAC16810.1495052807115064No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16450.14630350194552527No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC16340.14532518065591996No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG16160.1437242912729294No Hit
GATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTTCCGTGTTCGG16000.1423012784880489No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC15960.1419455252918288No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15900.1414118954974986No Hit
CCATAACCTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGACGGTGACCCACTGCCCTGGGGCCCCAGACA15490.13776542523624236No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15350.13652028904947192No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15000.13340744858254586No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14990.13331851028349084No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT14940.13287381878821566No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT14890.1324291272929405No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14820.1318065591995553No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14750.1311839911061701No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT14570.12958310172317955No Hit
GTGTCTGGGGCCCCAGGGCAGTGGGTCACCGTCTCCTGCACTGGGAGCAG14310.12727070594774875No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14270.12691495275152861No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14180.12611450806003335No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13920.12380211228460256No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13570.12068927181767648No Hit
GTGCAGGAGACGGTGACCCACTGCCCTGGGGCCCCAGACACTGAGGGCGG13260.11793218454697055No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13180.1172206781545303No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13040.11597554196775986No Hit
TCACTGGGCTCCAGGCTGAAGATGAGGCTGATTATTACTGCCAGTCCTAT12680.11277376320177877No Hit
GTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGTGGGTCACCGTCTCCTGCACTGGGAGCAG12510.11126181211784324No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12380.11010561423012784No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC12350.10983879933296274No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA12140.10797109505280712No Hit
GGGCAGTGGGTCACCGTCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGGTGTCCACTGGTACCAG12020.10690383546414675No Hit
GTATCAACGCAGAGTACGGGAGGCTCTGCTTCAGCTGTGGGCACAAGAGG11610.1032573652028905No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACGGAACCACTCAGGC11580.1029905503057254No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTG11580.1029905503057254No Hit
GTCTGGGGCCCCAGGGCAGTGGGTCACCGTCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGGTGT11480.1021011673151751No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAAGATG11470.10201222901612006No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11390.10130072262367981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3750.041.785921
GCAAGCG350.00144217738.88939725
ATAGACT350.001448498238.8548054
ACCGTTC400.00279039233.9964458
GTATCAA31850.033.6902731
GGTATCA15800.033.6334151
CGTTTCG450.003472590132.5121366
ATCAACG32650.031.9788253
TCAACGC32950.031.6763944
CAACGCA33550.031.1085175
AACGCAG34100.030.806166
TAGACTC450.004967319330.2190595
TATCAAC36500.029.4587362
CGCAGAG36200.029.1129748
TAACACG605.907649E-428.3316294
AGAGTAC37350.028.30761311
CAGAGTA37350.028.30761310
GACCGAC854.964686E-627.997077
ACGCAGA38150.027.5357847
TCTAGTA500.00827409927.2213652
Produced by FastQC (version 0.11.5)