FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences579405
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10090.1741441651349229No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC6560.11321959596482599No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT6450.11132109664224507No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAC150.002377308768.83575433
GGTATCA10100.044.204411
GTATCAA26600.040.928071
GGTACGG451.0281187E-438.1593671
TATCAAC30150.036.289732
ATCAACG29850.036.2783853
TCAACGC30000.035.9703374
CAACGCA30200.035.7259565
AACGCAG30400.035.6035926
TTCGATA501.8913165E-434.40296632
CGCAGAG32250.033.34888
GTGGTAT2900.033.159171
ACGCAGA33050.032.6452037
AGAGTAC33600.032.1108311
GCAGAGT33550.032.0565959
CAGAGTA34150.031.69397210
GAGTACG25150.029.82540712
AGTACGG25350.029.72521213
CCCGAAA754.4331566E-528.96338567
GCGGAAA500.00670600728.41646863
Produced by FastQC (version 0.11.5)