FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324982
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9050.043.373561
TAACCGT405.0566938E-543.0340237
TATCAAC10150.038.6729742
GGTATCA3800.037.1508751
ATCAACG10650.036.857343
TCAACGC10750.036.194184
CAACGCA11150.034.8957375
AACGCAG11350.034.58426
CTAACCG400.002618626734.4325186
CGCAGAG11650.033.3929258
TGGTATC1154.7293724E-1132.935452
ACGCAGA12100.032.435567
CAGAGTA12200.032.16969310
AGAGTAC12350.032.05773511
GCAGAGT12800.030.6617439
TTTACGG450.00466230230.60668423
ACGGGGA1600.030.12845416
GTGGTAT1401.4551915E-1129.5135841
AGTACGG10100.028.97340213
CTTTACG605.476132E-428.69376422
Produced by FastQC (version 0.11.5)