FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324982
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG150.00238221268.793174
TTAAGCG252.3444308E-456.21160
GTATCAA10400.040.4681661
TATCAAC11550.035.4771232
TCAACGC11600.035.286154
ATCAACG11650.035.1455273
CAACGCA11700.034.9791765
GGTATCA4100.034.4974561
GTCTTAT400.002594533834.4974561
GTGGTAT1700.034.497451
AACGCAG12000.034.3912936
ACCGCGT400.002634056134.391298
TATCCCG400.002634056134.391295
AGAGTAC12650.032.6241511
CGCAGAG12950.032.1339458
ACGCAGA12900.031.9919017
CAGAGTA12950.031.86837610
GCAGAGT13100.031.7669
TTACACC450.00468617530.574744
AGTACGG10750.030.07238613
Produced by FastQC (version 0.11.5)