FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1163052
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT59770.5139065149279654No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC54290.46678910315273947No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC47740.41047175878636555No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43720.3759075260607436No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC41520.3569917768079157No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38630.3321433607439736No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG38580.3317134573518639No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38250.3288760949639397No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35950.3091005389268924No Hit
GTAATATGTTGCAAGATCTTCAGGTTGCAGGCTGCTGATGGTGAGGGTAAAATTTGCCCCAGATCCACTTCCG35760.3074669060368754No Hit
ACATATTACTGTCAACAGTTTCATAATTTACCGATCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAA34640.2978370700536176No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG34370.29551559173622505No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG34170.29379597816778613No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC33560.2885511567840475No Hit
GTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG33160.2851119296471697No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT32320.2778895526597263No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31950.27470826755811434No Hit
ATATTACTGTCAACAGTTTCATAATTTACCGATCACTTTCGGCGGAGGGA30170.25940370679900815No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG30110.25888782272847644No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28670.2465066050357164No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG28510.24513091418096525No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG27990.24065991890302413No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27670.23790853719352187No Hit
GGGTAAAATTTGCCCCAGATCCACTTCCGCTGAACCTTGACGGGACCCCT26750.22999831477870294No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26660.2292244886729054No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG25620.22028249811702313No Hit
CACCATCAGCAGCCTGCAACCTGAAGATCTTGCAACATATTACTGTCAACAGTTTCATAATTTACCGATCACTTT24740.212716198415892No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24040.20669755092635583No Hit
GTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGAAATCATCAACTATTTAAATTGGTATCAGCAGCAA23600.20291440107579026No Hit
CAGTAATATGTTGCAAGATCTTCAGGTTGCAGGCTGCTGATGGTGAGGGTAAAATTTGCCCCAGATCCACTTCCG23510.20214057496999277No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22860.1965518308725663No Hit
CTCCTGATCTGCGATGCATCGAAATTGGAACCAGGGGTCCCGTCAAGGTT22340.1920808355946252No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC22260.19139299016724962No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21780.18726591760299627No Hit
ATTTTACCCTCACCATCAGCAGCCTGCAACCTGAAGATCTTGCAACATATTACTGTCAACAGTTTCATAATTTAC21770.1871799369245743No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21230.1825369802897893No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCC21050.1809893280781943No Hit
CCTTCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCA20890.17961363722344315No Hit
GCTGATGGTGAGGGTAAAATTTGCCCCAGATCCACTTCCGCTGAACCTTG20820.17901177247448954No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTGCTGCTGATACCAATTTAAAT20810.17892579179606757No Hit
GTCTCCTTCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGAAATCATCAA20570.1768622555139409No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA19940.17144547277335836No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19940.17144547277335836No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGGTGTGCCAT19890.17101556938124865No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC19690.16929595581280976No Hit
ATCTTCAGGTTGCAGGCTGCTGATGGTGAGGGTAAAATTTGCCCCAGATCCACTTCCGCTGAACCTTGACGGGAC19400.16680251613857333No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG19270.16568476731908804No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18930.16276142425274193No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18560.15958013915112995No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18260.1570007187984716No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18220.1566567960847838No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC18200.15648483472793995No Hit
GACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCT18100.1556250279437205No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17850.15347551098317186No Hit
GGGAAAGCCCCTAAGCTCCTGATCTGCGATGCATCGAAATTGGAACCAGG17620.15149795537946711No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT17620.15149795537946711No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC17590.15124001334420129No Hit
CCTGAAGATCTTGCAACATATTACTGTCAACAGTTTCATAATTTACCGAT17420.14977834181102823No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17360.14926245774049657No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17330.14900451570523074No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17300.14874657366996488No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17000.14616715331730654No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT16970.1459092112820407No Hit
GAAGTGGATCTGGGGCAAATTTTACCCTCACCATCAGCAGCCTGCAACCT16970.1459092112820407No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG16810.14453352042728956No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16710.14367371364307013No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16640.14307184889411653No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16470.14161017736094345No Hit
CCCCTAAGCTCCTGATCTGCGATGCATCGAAATTGGAACCAGGGGTCCCG16330.14040644786303622No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16180.13911673768670704No Hit
ATCCAGATGACCCAGTCTCCTTCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGT15950.1371391820830023No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGGTGTGCCATCCAGATGACCCAGTCTCCTTCCTCC15840.1361933946203609No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15810.13593545258509507No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15790.13576349122825118No Hit
GTTTCATAATTTACCGATCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCAT15590.1340438776598123No Hit
ATCTCCACCTTGGTCCCTCCGCCGAAAGTGATCGGTAAATTATGAAACTG15570.13387191630296838No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGGTGTGCCATCCAGATGACCCAGTCTCCTTC15520.13344201291085867No Hit
GGTGGAGATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC15510.13335603223243672No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT15310.13163641866399783No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT14910.12819719152712003No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC14840.1275953267781664No Hit
GATCTGCGATGCATCGAAATTGGAACCAGGGGTCCCGTCAAGGTTCAGCGGAAGTGGATCTGGGGCAAATTTTAC14410.12389815760602278No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA14370.123554234892335No Hit
GGTATCAGCAGCAACCAGGGAAAGCCCCTAAGCTCCTGATCTGCGATGCATCGAAATTGGAACCAGGGGTCCCGT14360.12346825421391304No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14160.12174864064547414No Hit
ACCTTGACGGGACCCCTGGTTCCAATTTCGATGCATCGCAGATCAGGAGCTTAGGGGCTTTCCCTGGTTGCTG14100.12123275657494248No Hit
GCTTAGGGGCTTTCCCTGGTTGCTGCTGATACCAATTTAAATAGTTGATG13990.12028696911230109No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13960.12002902707703525No Hit
CTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCA13950.11994304639861329No Hit
CCTTGGTCCCTCCGCCGAAAGTGATCGGTAAATTATGAAACTGTTGACAG13750.11822343283017439No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13710.11787951011648662No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC13460.115729993155938No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG13130.1128926307680138No Hit
CCATCAGCAGCCTGCAACCTGAAGATCTTGCAACATATTACTGTCAACAG13080.11246272737590408No Hit
ACCTTGGTCCCTCCGCCGAAAGTGATCGGTAAATTATGAAACTGTTGACA13070.11237674669748214No Hit
GCAGCAACCAGGGAAAGCCCCTAAGCTCCTGATCTGCGATGCATCGAAATTGGAACCAGGGGTCCCGTCAAGGTT12960.11143095923484075No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT12940.11125899787799685No Hit
GCCCCTAAGCTCCTGATCTGCGATGCATCGAAATTGGAACCAGGGGTCCCGTCAAGGTTCAGCGGAAGTGGAT12880.11074311380746518No Hit
GTTCAGCGGAAGTGGATCTGGGGCAAATTTTACCCTCACCATCAGCAGCC12770.1097973263448238No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT12640.10867957752533851No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG12640.10867957752533851No Hit
GGACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATC12460.10713192531374349No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12430.10687398327847766No Hit
GCCACAGTTCGCTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGATCGG12280.10558427310214849No Hit
GTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGGTGTGCCATCCAGATGACCCAGTCT12220.10506838903161682No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12150.1044665242826632No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12130.1042945629258193No Hit
GAGTCACCATCACTTGCCAGGCGAGTCAGGAAATCATCAACTATTTAAATTGGTATCAGCAGCAACCAGGGAAAG11970.1029188720710682No Hit
CACCATCACTTGCCAGGCGAGTCAGGAAATCATCAACTATTTAAATTGGTATCAGCAGCAACCAGGGAAAGCCC11940.10266093003580236No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11810.10154318121631707No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGCT150.002367315368.91337633
CGTATGC1100.044.072441
GTATCAA22200.041.5015641
TCGTATG1200.040.36822540
GTGGTAT2500.039.8786661
ACGAGAC1200.037.24460622
TATCAAC25350.036.0702322
ATCTCGT1350.035.8038437
ATCAACG26050.035.2329333
CAACGCA26350.035.0927125
TCAACGC26400.034.8960384
GGTACGG400.00264289534.3752063
AACGCAG27200.033.9960676
CTTATAC1800.032.468261
TATACAC1700.032.3531343
AATCTCG1500.032.2012136
AGAGTAC29050.031.82697911
ACGGGAT1305.456968E-1231.7282316
CGCAGAG29350.031.5016618
CAGAGTA30150.030.66579610
Produced by FastQC (version 0.11.5)