FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684812
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16150.23583114781867137No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACAAAAGTGAAGCTGCCTGCATATGGGCAGCAGTAATAAT14420.21056874003376108No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14110.2060419501994708No Hit
GATTATTACTGCTGCCCATATGCAGGCAGCTTCACTTTTGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGT13880.20268336419338445No Hit
GTAATAATCAGCCTCATCGTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG12460.18194774624276444No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12260.17902723667225456No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACAAAAGTGAAGCTGCCTGCATATGGGCAG11820.17260211561713287No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11440.16705314743316413No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC11220.1638405869056033No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10640.15537110915112468No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG10530.15376482888734427No Hit
TCTCTGGGCTCCAGGCTGACGATGAGGCTGATTATTACTGCTGCCCATAT10460.1527426505376658No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10220.14923803905305397No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG10180.148653937138952No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG9600.1401844593844734No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGAACTGATGTT9510.13887023007774396No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9410.13740997529248902No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT9310.13594972050723408No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9180.13405138928640267No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8940.13054677780179086No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8530.12455973318224564No Hit
GTAATATATAGCCGTGTCTGCGGCGGTCATAGACCTCAGCTTCAGGGAGA8470.12368358031109268No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG8270.12076307074058283No Hit
CCCCTGAGGGCCGCTTATTGACATCATAAATCATGAGTTTGGGGGCTTTGCCTGGCTGTTGTTGGTACCAGGAGA8190.11959486691237887No Hit
CTCCTGCACTGGAACCAGAACTGATGTTGGTGGTTATAAGTATGTCTCCTGGTACCAACAACAGCCAGGCAAAGC8040.11740448473449648No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC8020.11711243377744548No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC7960.11623628090629252No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7820.11419192420693562No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC7790.11375384777135916No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT7780.11360782229283366No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7370.10762077767328844No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT7280.10630654836655899No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG7270.1061605228880335No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTC7180.10484629358130405No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG7020.10250988592489618No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6940.10134168209669223No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA6900.10075758018259026No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAA352.3179271E-549.1188431
GGTATCA6150.041.3716471
GTATCAA15000.040.8013841
GTGGTAT1750.037.3303221
TATCAAC17500.035.120242
TACGGAT3650.034.92630470
ATCAACG17900.033.9245073
TCAACGC17850.033.8125154
TGGTATC1950.033.4550322
CAACGCA18150.033.25125
AACGCAG18700.032.8233386
ATCGTTT450.004197897431.27493157
GCGCTTA450.004742614530.50476826
CTAGGTA450.004759591530.4824944
CGTATAT703.2055974E-530.37414762
AATTAGG850.00514652229.99552770
AGAGTAC20650.029.55774511
CGCAGAG20700.029.486358
CAGAGTA21000.029.06511510
GCAGAGT21400.028.3616079
Produced by FastQC (version 0.11.5)