FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452709
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19240.42499707317504176No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA14750.32581636327088703No Hit
GTATATTACTGTCAGCATTATGGTGGTTCACCTCCTCTCACTTTCGGCGG12470.2754528847449465No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11420.25225917752905286No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11200.24739954363619895No Hit
GTAATATACGGCCGTGTCCGCAACGGTCACAGAGTTCAGATTCAGGGAGAACTGGTTGTTGGACGTGTCTGCCGA10770.23790116830016633No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10000.22089244967517765No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9770.21581192333264856No Hit
GTCTCTGGTGGCTCCATCAGTGGTTACTATTGGGGCTGGATCCGGCAGCC9660.2133821063862216No Hit
ATATTACTGTCAGCATTATGGTGGTTCACCTCCTCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAA9600.21205675168817054No Hit
GTAATATACCGCAAAGTCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCGCTGCCA9410.20785979514434216No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8490.18753768977422583No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8460.1868750124252003No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8360.18466608792844852No Hit
GTGTTAGTAGCATTCACCTGGCCTGGTACCAGCAGAGACCTGGCCATGCTCCCAGGCTCCTCATTCATAGTGCAT8010.1769348521898173No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGTGAGAGGAGGTGAACCACCATAATG7640.16876183155183572No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAGCATTCACCTGGCCTGGTACC7590.16765736930345984No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7520.1661111221557336No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7170.15837988641710238No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGACTTTGCGGTATATTACTGTCAGCATTATGGTGGTTCA6750.14910240353074491No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC6710.14821883373204423No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT6680.14755615638301867No Hit
CCATAATGCTGACAGTAATATACCGCAAAGTCCTCAGGCTCCAGTCTGCT6600.14578901678561723No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6590.14556812433594207No Hit
CAGTAATATACCGCAAAGTCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCGCTGCCA6580.14534723188626691No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6520.14402187718821582No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT6490.1433591998391903No Hit
GTATATTACTGTGCGAGACTCGATAGTGGAAACGATCGACACGGCTATTATTACTACTACCACGGTTTGGACGT6440.14225473759081442No Hit
GCATTATGGTGGTTCACCTCCTCTCACTTTCGGCGGAGGGACCAGGGTGG6390.1411502753424385No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6320.1396040281947123No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6320.1396040281947123No Hit
CTCCAGGCTCCCTGTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGC6270.1384995659463364No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC6120.13518617920120873No Hit
GTCCCAGACCCGCTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCG6090.13452350185218317No Hit
GTCTCCAGGCTCCCTGTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAG6010.13275636225478177No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT6010.13275636225478177No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC5970.13187279245608105No Hit
CTCCCAGGCTCCTCATTCATAGTGCATCCCGCAGGGCCACTGGCATCCCA5740.12679226611355196No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG5710.12612958876452643No Hit
GTCCTGGACCTCCTGTGCAAGAGCATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC5630.12436244916712502No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5610.12392066426777466No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG5580.12325798691874912No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAGTTGTGTTGACGCAGTC5560.12281620201939879No Hit
CATTCATAGTGCATCCCGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGCGGGTCTGGGACAGACTTCA5510.12171173977102288No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC5490.12126995487167253No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCC5380.11884013792524557No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGACT5270.11641032097881862No Hit
GTACCAGCAGAGACCTGGCCATGCTCCCAGGCTCCTCATTCATAGTGCATCCCGCAGGGCCACTGGCATCCCAGA5240.11574764362979309No Hit
GTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA5210.11508496628076754No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT5090.11243425688466542No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG4980.11000443993823847No Hit
TTCATAGTGCATCCCGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGCGGGTCTGGGACAGACTTCACT4960.10956265503888811No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA4860.10735373054213634No Hit
GAGTGAAGTCTGTCCCAGACCCGCTGCCACTGAACCTGTCTGGGATGCCA4850.10713283809246116No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG4750.10492391359570939No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4730.10448212869635903No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG4720.10426123624668385No Hit
GGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAGCATTCACCTGGCCTGGTACCAGCAGAGAC4720.10426123624668385No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA4700.10381945134733349No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT4670.10315677399830796No Hit
CCTCATTCATAGTGCATCCCGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGCGGGTCTGGGACAGACTT4630.10227320419960725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9650.043.3660241
GGTATCA3400.042.723021
TATCAAC11150.036.8585852
CACCGAC400.002546769334.63133639
TCAACGC12000.034.4859734
CGGTACT400.002599859434.485974
TAAACTC400.002601268234.4821625
ATCAACG12050.034.3618553
AACGCAG12400.033.3698356
CAACGCA12550.032.9709935
TGGATTG5250.032.5219370
ATTAGAG652.3161241E-531.8507963
ACGGGAT1305.456968E-1231.83320616
AGAGTAC13100.031.58671211
CGGGATT553.2617018E-431.35088517
ACGCAGA13250.031.229137
GCAGAGT13450.031.0211289
GGGATAC901.9977779E-730.65419618
CAGAGTA13650.030.56660710
CGCAGAG13600.030.4254388
Produced by FastQC (version 0.11.5)