FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences745061
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38000.5100253536287633No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA30220.40560437333319016No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23440.3146051128699529No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCGGCCTAGAGCCTGAAGATT23300.3127260720934259No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22420.30091495864097034No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21690.29111710316336514No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20390.2736688673813285No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAT20160.2705818718198913No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC19700.26440788069701676No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19140.2568917175909087No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT18760.25179146405462105No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18670.2505835092697108No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17840.23944348180887204No Hit
GTTCTGACGGTGCTGACAGTAATAAACTGCAGAATCTTCAGGCTCTAGGCCGCTGATGGTGAGAGTGAAGT17650.2368933550407282No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17100.2295114091329435No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCATCTACTTAGCCTGGTACCAACAGAAACCTGACCAGGCTCCCAGGCT16390.21998198805198502No Hit
GTTTATTACTGTCAGCACCGTCAGAACTGGCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA15510.20817087459952943No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG15420.2069629198146192No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15360.20615761662467905No Hit
GAGTGTTACCATCTACTTAGCCTGGTACCAACAGAAACCTGACCAGGCTCCCAGGCTCCTCATCTATGATGCATC14800.19864145351857096No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14640.1964939783453972No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14570.19555445795713372No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14450.1939438515772534No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCATCTACTTAGCCTGGTA14330.1923332451973731No Hit
GACTTCACTCTCACCATCAGCGGCCTAGAGCCTGAAGATTCTGCAGTTTATTACTGTCAGCACCGTCAGAACTGG14190.1904542044208461No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14000.18790407765270226No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT13910.18669612286779202No Hit
GTAATAAACTGCAGAATCTTCAGGCTCTAGGCCGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA13730.18428021329797156No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA13710.18401177890132486No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT13100.17582452980359997No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGCCAGTT13040.17501922661365982No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA12190.16361076475617434No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11510.15448399527018594No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11350.15233652009701218No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11210.15045747932048517No Hit
GCCTAGAGCCTGAAGATTCTGCAGTTTATTACTGTCAGCACCGTCAGAACTGGCCGTGGACGTTCGGCCAAGGGA11020.14790735255234133No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10990.14750470095737128No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10970.14723626656072455No Hit
GTCAGAGTGTTACCATCTACTTAGCCTGGTACCAACAGAAACCTGACCAGGCTCCCAGGCTCCTCATCTATGATG10730.14401505380096394No Hit
ACAGAAACCTGACCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTT10680.14334396780934713No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10370.13918323466132304No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10280.1379752798764128No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10130.13596202190156242No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC10060.13502250151329892No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9990.1340829811250354No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT9960.1336803295300653No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC9880.13260659194347846No Hit
CGTCAGAACTGGCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT9830.13193550595186165No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG9760.13099598556359815No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC9740.13072755116695145No Hit
CATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA9540.12804320720048426No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9390.12602994922563387No Hit
GTGTTACCATCTACTTAGCCTGGTACCAACAGAAACCTGACCAGGCTCCC9060.12160078168096304No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8960.12025860969772945No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8910.11958752370611264No Hit
CACCATCAGCGGCCTAGAGCCTGAAGATTCTGCAGTTTATTACTGTCAGCACCGTCAGAACTGGCCGTGGACGTT8810.11824535172287906No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8700.11676896254132212No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCGGCC8690.11663474534299877No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8470.11368196697988486No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8470.11368196697988486No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC8400.11274244659162135No Hit
CAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCGG8220.1103265370218009No Hit
GGACTACAGTAACAAGCGTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGGAGTGCATCCGCCC8210.11019231982347755No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC7930.10643423827042349No Hit
CTACTTAGCCTGGTACCAACAGAAACCTGACCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCA7830.1050920662871899No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT7820.10495784908886654No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCATCTACTTAGCCTGGTACCAACAGAAACCTGACCAGG7680.10307880831233952No Hit
GAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTC7670.10294459111401617No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7640.1025419395190461No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7580.10173663632910594No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC7570.10160241913078258No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7550.10133398473413585No Hit
GCCTGGTACCAACAGAAACCTGACCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATC7530.10106555033748915No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)