FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450461
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20880.4635251442411219No Hit
CTATAGCAGCATTCCGTACTCTTTTGGCCAGGGGACCACGTTGGAGATCAAACGAACTGTGGCTGCACCAT18320.4066944752153905No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17490.3882689067422041No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14820.3289962949067733No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG14260.3165645860573945No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14220.3156766068538675No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13440.29836101238508994No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13200.29303313716392765No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12970.28792725674364705No Hit
GTGCAAGTGCATACGGGTCTGGGACAGATTTTACTTTCACCATCAGCAGC12160.26994567787222423No Hit
CTTTTGGCCAGGGGACCACGTTGGAGATCAAACGAACTGTGGCTGCACCA11620.257957958624609No Hit
CTGCTATAGTGTTGACAGTAATATGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTC11400.25307407300521023No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11030.24486026537258498No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10800.23975438495230442No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10550.23420451493026034No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10470.23242855652320624No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10460.23220656172232446No Hit
GTAATATGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAA10270.22798866050557096No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10160.22554671769587156No Hit
CACTTGCACTGAACCTTGATGGGACCCCTGTTTGCAAATTGGATGCCTCGTAGATCAGGAGTTTAGGGGCTGTCC9850.2186648788685369No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9680.21489096725354692No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9570.21244902444384753No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9510.21111705563855696No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9210.20445721161210403No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9000.19979532079358703No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAACGTGGTCCCCTGGCCAAAAGAG8930.19824135718741467No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC8820.19579941437771528No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC8410.18669762754156297No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8250.18314571072745475No Hit
GCCCCTAAACTCCTGATCTACGAGGCATCCAATTTGCAAACAGGGGTCCC8240.182923715926573No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8200.18203573672304596No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8170.18136975232040065No Hit
GTAATAGACGGCCGTGTCCTCAGCTCTCAGGTTGTTCATTTGCAGATACA8160.1811477575195189No Hit
ATATTACTGTCAACACTATAGCAGCATTCCGTACTCTTTTGGCCAGGGGA7940.1762638719001201No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7930.17604187709923833No Hit
GGCCAAAAGAGTACGGAATGCTGCTATAGTGTTGACAGTAATATGTTGCA7840.17404392389130247No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7820.17359993428953893No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7780.1727119550860119No Hit
CAGTAATATGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGACCCGTATGCA7770.1724899602851301No Hit
ACATATTACTGTCAACACTATAGCAGCATTCCGTACTCTTTTGGCCAGGGGACCACGTTGGAGATCAAACGAA7750.1720459706833666No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7590.1684940538692584No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT7550.16760607466573132No Hit
GGTCTGGGACAGATTTTACTTTCACCATCAGCAGCCTGCAGCCTGAAGAT7510.16671809546220429No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7340.1629441838472143No Hit
GTCTATTACTGTGCGAGACCCCGGGGAGTCTCCTATTATTACTACGGTAT7340.1629441838472143No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7210.16005825143575136No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC7150.1587262826304608No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAACGTGGTCCCCTGGCCAAAAGAGTACGGAATG6970.15473037621458904No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT6910.15339840740929847No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT6670.14807053218813615No Hit
GTTTAGGGGCTGTCCCTGGTTTCTTCTGATACCAATTTAAATAGGTGCTA6620.14696055818372733No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA6570.1458505841793185No Hit
ACCAGGGACAGCCCCTAAACTCCTGATCTACGAGGCATCCAATTTGCAAA6530.14496260497579147No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC6460.14340864136961912No Hit
GTCCCAGACCCGTATGCACTTGCACTGAACCTTGATGGGACCCCTGTTTG6440.1429646517678556No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATAGTCAC6420.14252066216609208No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6360.14118869336080148No Hit
GTCTGCATCTGTAGGAGACACAGTCACCATCACTTGCCAGGCGAGTCAGG6210.13785877134757504No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG6150.13652680254228447No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6120.13586081813963918No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT6110.1356388233387574No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT6070.13475084413523036No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA5960.13230890132553097No Hit
AGTCAGGACATTAGCACCTATTTAAATTGGTATCAGAAGAAACCAGGGAC5940.13186491172376744No Hit
GAAGAAACCAGGGACAGCCCCTAAACTCCTGATCTACGAGGCATCCAATTTGCAAACAGGGGTCCCATCAAGGTT5920.1314209221220039No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC5850.12986695851583155No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCGTC5820.12920097411318626No Hit
GTAGGAGACACAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCACCTATTTAAATTGGTATCAGAAGAAA5810.1289789793123045No Hit
GTCTCCGTCCTCCCTGTCTGCATCTGTAGGAGACACAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAC5780.12831299490965922No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG5720.12698102610436862No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA5680.12609304690084158No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCGTCCTCC5660.12564905729907805No Hit
GCTATAGTGTTGACAGTAATATGTTGCAACATCTTCAGGCTGCAGGCTGC5640.12520506769731454No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT5590.12409509369290571No Hit
TCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGACCC5580.12387309889202394No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG5460.1212091612814428No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT5440.12076517167967926No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5430.1205431768787975No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC5420.12032118207791573No Hit
CTCCTGATCTACGAGGCATCCAATTTGCAAACAGGGGTCCCATCAAGGTT5400.11987719247615221No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC5340.11854522367086162No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT5330.11832322886997987No Hit
GTGCATACGGGTCTGGGACAGATTTTACTTTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACATATTA5240.116325275662044No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC5170.11477131205587165No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA5160.11454931725498989No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT5140.11410532765322637No Hit
CACTATAGCAGCATTCCGTACTCTTTTGGCCAGGGGACCACGTTGGAGATCAAACGAACTGTGGCTGCACCAT5130.1138833328523446No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC5110.11343934325058107No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG5080.11277335884793578No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT5070.11255136404705401No Hit
GCCTGAAGATGTTGCAACATATTACTGTCAACACTATAGCAGCATTCCGT5010.11121939524176344No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG5000.11099740044088167No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA4980.11055341083911815No Hit
GATTTTACTTTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACATATTACTGTCAACACTATAGCAGCATT4970.11033141603823639No Hit
GTTCAGTGCAAGTGCATACGGGTCTGGGACAGATTTTACTTTCACCATCA4960.11010942123735462No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA4780.10611351482148287No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT4770.10589152002060112No Hit
GTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCT4740.10522553561795583No Hit
CCCGTATGCACTTGCACTGAACCTTGATGGGACCCCTGTTTGCAAATTGG4650.10322758241001996No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC4610.10233960320649291No Hit
CCCCTAAACTCCTGATCTACGAGGCATCCAATTTGCAAACAGGGGTCCCA4610.10233960320649291No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC4580.10167361880384762No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA4560.10122962920208409No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGA4530.1005636447994388No Hit
CTCCAGAGACAGTTCCAAGAACACTCTGTATCTGCAAATGAACAACCTGAGAGCTGAGGACACGGCCGTCTATTA4520.10034164999855703No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)