FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450461
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20850.4628591598384766No Hit
CTATAGCAGCATTCCGTACTCTTTTGGCCAGGGGACCACGTTGGAGATCAAACGAACTGTGGCTGCACCAT16910.3753932082910618No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15580.3458678997737873No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG14960.33210422211911794No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14720.32677634689795565No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14020.3112367108362322No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12340.27394158428809595No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12310.2732755998854507No Hit
CTGCTATAGTGTTGACAGTAATATGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTC12260.2721656258810419No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10920.2424183225628856No Hit
CTTTTGGCCAGGGGACCACGTTGGAGATCAAACGAACTGTGGCTGCACCA10920.2424183225628856No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10910.2421963277620038No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10130.22488073329322628No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10100.224214748890581No Hit
GTGCAAGTGCATACGGGTCTGGGACAGATTTTACTTTCACCATCAGCAGC9990.2217728060808816No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9810.21777689966500985No Hit
GTAATATGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAA9730.21600094125795574No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9700.21533495685531046No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9530.21156104524032046No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9490.21067306603679342No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9390.20845311802797578No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9270.20578918041739463No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9210.20445721161210403No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAACGTGGTCCCCTGGCCAAAAGAG9000.19979532079358703No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8740.19402345597066117No Hit
CACTTGCACTGAACCTTGATGGGACCCCTGTTTGCAAATTGGATGCCTCGTAGATCAGGAGTTTAGGGGCTGTCC8650.1920255027627253No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8600.19091552875831647No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC8210.1822577315239277No Hit
GCCCCTAAACTCCTGATCTACGAGGCATCCAATTTGCAAACAGGGGTCCC8180.18159174712128243No Hit
GGCCAAAAGAGTACGGAATGCTGCTATAGTGTTGACAGTAATATGTTGCA7770.1724899602851301No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7680.17049200707719425No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT7650.16982602267454897No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC7610.1689380434710219No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7470.16583011625867722No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7450.16538612665691368No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7410.16449814745338665No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7330.16272218904633254No Hit
ACATATTACTGTCAACACTATAGCAGCATTCCGTACTCTTTTGGCCAGGGGACCACGTTGGAGATCAAACGAA7300.16205620464368725No Hit
ATATTACTGTCAACACTATAGCAGCATTCCGTACTCTTTTGGCCAGGGGA7240.16072423583839668No Hit
CAGTAATATGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGACCCGTATGCA7140.15850428782957904No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7070.15695032422340668No Hit
GGTCTGGGACAGATTTTACTTTCACCATCAGCAGCCTGCAGCCTGAAGAT7040.1562843398207614No Hit
GTAATAGACGGCCGTGTCCTCAGCTCTCAGGTTGTTCATTTGCAGATACA7010.1556183554181161No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA6840.15184444380312612No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA6780.15051247499783554No Hit
GTTTAGGGGCTGTCCCTGGTTTCTTCTGATACCAATTTAAATAGGTGCTA6710.14895851139166322No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT6700.14873651659078144No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT6640.14740454778549086No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6590.14629457378108204No Hit
GTCTATTACTGTGCGAGACCCCGGGGAGTCTCCTATTATTACTACGGTAT6580.1460725789802003No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6540.14518459977667322No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT6470.1436306361705009No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6460.14340864136961912No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC6260.13896874535198386No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT6200.13763677654669326No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATAGTCAC6190.1374147817458115No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6080.13497283893611212No Hit
ACCAGGGACAGCCCCTAAACTCCTGATCTACGAGGCATCCAATTTGCAAA5980.13275289092729448No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC5920.1314209221220039No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAACGTGGTCCCCTGGCCAAAAGAGTACGGAATG5900.13097693252024037No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA5890.13075493771935862No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG5880.13053294291847684No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCGTCCTCC5850.12986695851583155No Hit
GTCCCAGACCCGTATGCACTTGCACTGAACCTTGATGGGACCCCTGTTTG5820.12920097411318626No Hit
GTGCATACGGGTCTGGGACAGATTTTACTTTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACATATTA5780.12831299490965922No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA5780.12831299490965922No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT5750.12764701050701394No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT5740.12742501570613216No Hit
GTCTGCATCTGTAGGAGACACAGTCACCATCACTTGCCAGGCGAGTCAGG5730.1272030209052504No Hit
AGTCAGGACATTAGCACCTATTTAAATTGGTATCAGAAGAAACCAGGGAC5720.12698102610436862No Hit
GTCTCCGTCCTCCCTGTCTGCATCTGTAGGAGACACAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAC5650.1254270624981963No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC5600.12431708849378748No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT5550.12320711448937866No Hit
TCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGACCC5540.1229851196884969No Hit
GTAGGAGACACAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCACCTATTTAAATTGGTATCAGAAGAAA5530.12276312488761514No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC5480.12165315088320632No Hit
GCTATAGTGTTGACAGTAATATGTTGCAACATCTTCAGGCTGCAGGCTGC5390.11965519767527044No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA5380.11943320287438869No Hit
CTCCTGATCTACGAGGCATCCAATTTGCAAACAGGGGTCCCATCAAGGTT5270.11699126006468928No Hit
GAAGAAACCAGGGACAGCCCCTAAACTCCTGATCTACGAGGCATCCAATTTGCAAACAGGGGTCCCATCAAGGTT5250.11654727046292576No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCGTC5180.11499330685675341No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5130.1138833328523446No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5070.11255136404705401No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT5050.1121073744452905No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA5040.11188537964440873No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT4970.11033141603823639No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG4960.11010942123735462No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC4960.11010942123735462No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA4930.10944343683470933No Hit
GGGGACCACGTTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCA4900.10877745243206405No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA4880.10833346283030051No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC4870.10811146802941875No Hit
CCCCTAAACTCCTGATCTACGAGGCATCCAATTTGCAAACAGGGGTCCCA4820.10700149402500994No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA4770.10589152002060112No Hit
GATTTTACTTTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACATATTACTGTCAACACTATAGCAGCATT4760.10566952521971935No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA4730.10500354081707407No Hit
GTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCT4720.1047815460161923No Hit
GCCTGAAGATGTTGCAACATATTACTGTCAACACTATAGCAGCATTCCGT4690.10411556161354701No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG4610.10233960320649291No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA4560.10122962920208409No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT4560.10122962920208409No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT4540.10078563960032055No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT4540.10078563960032055No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC4520.10034164999855703No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTTG150.00221111170.10264660
TCGACGT150.002377173668.83427412
TTATAGG200.00738088351.7060631
GTGGTAT751.6370905E-1146.0120931
GTATCAA6350.042.9325451
TATCAAC7450.036.528462
ATCAACG7800.034.8816153
TACTGGG400.002614027434.4477272
CATTCAT400.002616877234.44007526
CAACGCA8000.034.4171375
TCAACGC7950.034.2006764
AACGCAG8150.033.783696
CGCAGAG8750.031.860448
CAGAGTA8950.031.14847210
GCAGAGT9350.029.8159169
TGCACAG3000.029.56610770
TACGGGC1103.731111E-828.1657315
GAGCGAG500.00759093527.70283742
GTACTGG500.007720076527.6072561
GTCATCG500.007808541427.54288117
Produced by FastQC (version 0.11.5)