FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218606
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46822.141752742376696No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT3930.1797754864916791No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC3350.15324373530461194No Hit
GTATATTACTGTCAGCATTATGGTGGTTCACCTCCTCTCACTTTCGGCGG3120.14272252362698187No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC3010.13769063978115878No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC2950.1349459758652553No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA2800.12808431607549656No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA2510.11481844048196299No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG2490.11390355250999515No Hit
ATATTACTGTCAGCATTATGGTGGTTCACCTCCTCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAA2370.10841422467818816No Hit
GTAATATACCGCAAAGTCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCGCTGCCA2260.10338234083236508No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT2240.10246745286039724No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTCG350.005444332555.813770
AGCGATG200.007585731351.33998539
GGGAATC200.00827483350.22338518
ATAACAC200.00828232550.211893
TAGGACG200.008304832550.1774375
TTACCCC200.008304832550.1774374
GGTATCA2400.043.3969421
GTATCAA5650.038.6521451
ACGGGAA559.0750345E-636.50938416
ACGGGAT701.2287619E-633.46693416
TCAACGC6500.032.4223444
ATCAACG6550.032.1969383
TACGGGA2200.031.93840415
CAACGCA6600.031.9310955
TATCAAC6800.031.070122
AACGCAG6800.030.9919476
ATACGGC652.8559654E-530.8784246
CGCAGAG6950.030.3230538
AGAGTAC7050.029.8929411
TCCGACA500.005419845729.67294160
Produced by FastQC (version 0.11.5)