FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences886491
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26470.29859299191982774No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20730.23384332159040533No Hit
GAGTTACCATCACTTGCCGGGCAAGTCAGACCATCAGCGGCTATTTAAATTGGTATCAGCAAAAACCTGGGAAAG19930.2248189772936217No Hit
GTTACAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA18910.21331293831522263No Hit
GTATTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT18370.20722150591489366No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17110.19300816364745949No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16650.1878191656768089No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16090.18150212466906038No Hit
ACAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA15740.17755397403921755No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15620.17620032239470002No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15370.17338021480195512No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA14900.16807841252759476No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13890.15668517785290542No Hit
GAGTTACAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT13280.14980411532660795No Hit
GAGTATTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT12810.1445023130522476No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12590.14202061837063207No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGGGACAGAGTTACCATCACTTGCCGGGCAAGTCAGACCATCAGCGG12280.13852368495562842No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12210.13773405482965986No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG11910.13434992571836601No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11870.13389870850352684No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11770.13277066546642888No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11730.1323194482515897No Hit
GGCCAAAAGTGTACGGAGTATTGTAACTCTGTTGACAGTAGTAAGTTGCA11290.12735605888835871No Hit
GTAGGGGACAGAGTTACCATCACTTGCCGGGCAAGTCAGACCATCAGCGGCTATTTAAATTGGTATCAGCAAAAA11270.1271304502809391No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG11240.12679203736980973No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10910.12306949534738651No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10830.12216706091770814No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10820.12205425661399834No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10580.11934695332496326No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG10490.1183317145915751No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC10230.11539880269512041No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAATACT10060.1134811295320539No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9770.11020980472446985No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA9530.10750250143543476No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT9340.10535921966494866No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC9270.10456958953898009No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG900.053.8940840
CTCGTAT1150.042.1540739
GTATCAA20450.041.4607771
TATCAAC22150.037.9653362
ATCAACG22900.037.0229263
TCAACGC23150.036.4742364
CAACGCA23250.036.4655955
CGTATGC1350.035.94161641
AACGCAG24000.035.3260426
ACGCAGA24800.034.0436827
ATGCCGT1450.033.51992844
AGAGTAC25950.032.26941311
CGCAGAG26200.032.2245568
ACTAGGC1500.032.18314427
TATACAC1850.031.6700213
ACGAGAC1650.031.33130322
GCAGAGT26950.031.3277689
TACGGAT1552.1798951E-730.35225170
CAGAGTA28800.029.1957410
TTATACA2250.029.10332
Produced by FastQC (version 0.11.5)