FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences886491
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25890.2920503423046596No Hit
GAGTTACCATCACTTGCCGGGCAAGTCAGACCATCAGCGGCTATTTAAATTGGTATCAGCAAAAACCTGGGAAAG19510.2200811965378103No Hit
GTATTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT19150.21602024160425767No Hit
GTTACAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA18950.2137641555300618No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17040.1922185335214909No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17020.19199292491407133No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16120.18184053758018975No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15450.1742826492316335No Hit
ACAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA14920.16830402113501433No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14720.16604793506081844No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14010.15803882949742298No Hit
GAGTATTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT13960.157474807978874No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA13700.15454189608241933No Hit
GAGTTACAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT13530.15262422291935282No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11960.13491394723691497No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG11780.13288346977013868No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGGGACAGAGTTACCATCACTTGCCGGGCAAGTCAGACCATCAGCGG11760.13265786116271908No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11760.13265786116271908No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11740.1324322525552995No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11680.13175542673304072No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11540.13017616648110358No Hit
GTAGGGGACAGAGTTACCATCACTTGCCGGGCAAGTCAGACCATCAGCGGCTATTTAAATTGGTATCAGCAAAAA11450.12916092774771543No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11230.12667923306609993No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG10940.12340790825851589No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10840.12227986522141794No Hit
GGCCAAAAGTGTACGGAGTATTGTAACTCTGTTGACAGTAGTAAGTTGCA10800.12182864800657875No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10750.12126462648802977No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10650.12013658345093182No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10640.12002377914722202No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9930.11201467358382658No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC9830.11088663054672862No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA9780.11032260902817964No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG9730.10975858750963068No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAATACT9540.10761530573914456No Hit
GTCTGCATCTGTAGGGGACAGAGTTACCATCACTTGCCGGGCAAGTCAGA9430.10637445839833681No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG200.007474844351.545046
GTATCAA20050.044.6718331
TATCAAC23050.039.1145942
TCAACGC23050.038.912554
CAACGCA23200.038.6587755
ATCAACG23400.038.341343
AACGCAG24250.036.9848946
ACGCAGA25700.035.0319067
AGAGTAC26400.034.1030311
TACGGAT1606.157279E-933.89286470
CGCAGAG26850.033.6594548
CGACCGG953.049975E-431.71262470
GCAGAGT28650.031.6646659
GCTTACG450.004280692431.15140556
CAGAGTA29250.031.01513510
AGTACGG22650.029.2809213
CGTGTCA1302.0736479E-1029.07668913
GAGTACG23500.028.22182312
TCCTATA1005.128877E-727.5295072
CGTTTCG1155.278889E-827.18284843
Produced by FastQC (version 0.11.5)