FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337814
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT3640.10775160295310438No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCGA200.006950550752.49458758
GTATCAA10700.041.232691
GGTATCA4650.039.286051
TCAACGC11400.038.700864
ATCAACG11700.038.592323
CAACGCA12000.037.0530435
TTGCGGA650.00185348636.93751570
TATCAAC12050.036.89932
AACGCAG12600.035.2886126
ACGCAGA12650.034.87157
AGAGTAC13400.033.17691811
CAGAGTA13550.032.80964710
CGCAGAG13550.032.555318
TACGGGC3000.032.16532515
GCAGAGT13950.031.6218249
CGAAATT1001.29693944E-831.39294453
CTCGCTA450.004635802530.64271523
TAAGCGC450.004635802530.64271523
ACGGGCG902.0070001E-730.63364416
AGCGTCA604.9448136E-429.1987759
Produced by FastQC (version 0.11.5)