FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337814
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8010.23711273067427638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10050.044.080081
CTCGCTA451.00320416E-438.3129323
CAACGCA11850.037.2349625
GGTATCA4100.037.142171
ATCAACG11850.036.9714283
TCAACGC11900.036.7997324
AACGCAG12200.036.166756
TATCAAC12500.035.387792
GTGGTAT1500.034.609751
ACGCAGA12650.034.607687
CGCAGAG13100.033.418878
AGAGTAC13050.033.2827611
AGTTGCG450.003324007432.7966568
CAGAGTA13500.032.4286810
TCGCTAT553.2595315E-431.35158724
CCGTTAA553.2651983E-431.34230416
GCAGAGT14000.031.2705159
GTCGAGT450.004633513330.64581116
AGTACGG11550.030.44230513
AGTTCGG1004.004879E-430.26104470
Produced by FastQC (version 0.11.5)