FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197723
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10260.5189077649034255No Hit
GTATGATAGTTATTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCGCACGAACTGTGGCTGCACCATCTGTCTT8230.4162388796447556No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA7440.3762839932633027No Hit
CTATCATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC7200.3641457999322284No Hit
CTCCTGTACAGCCCCTGGATTCACCCTCAACAACTTTGGCATGCACTGGGCCCGCCAGGCTCCAGGCAAGGGG7160.3621227677103827No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC6960.35200760660115415No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC6790.34340971965830985No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6590.3332945585490813No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG6530.3302600102163127No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT6270.31711030077431557No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6260.3166045427188542No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6170.3120527202197013No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG5480.27715541439286273No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA5350.2705805596718642No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT4970.2513617535643299No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT4870.2463041730097156No Hit
GTGCAGCCACAGTTCGTGCGATTTCCACCTTGGTCCCTTGGCCGAACGTC4810.24326962467694704No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT4770.24124659245510133No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT4670.23618901190048702No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT4440.22455657662487422No Hit
GAATATTGATAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTAATCTATAAGGCGT4400.2225335444030285No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG4360.22051051218118278No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC4330.21899323801479847No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC4300.2174759638484142No Hit
GCCACAGTTCGTGCGATTTCCACCTTGGTCCCTTGGCCGAACGTCCAATA4290.21697020579295279No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT4260.21545293162656848No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC4240.2144414155156456No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTGCGATTTCCACCTTGGTCC4130.2088780769055699No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAATATTGATAGCTGGTTGGCCTGGTATCAGCAGAAA4130.2088780769055699No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG4100.20736080273918564No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACT4030.20382049635095564No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC3920.1982571577408799No Hit
CCTTCCACTCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCG3920.1982571577408799No Hit
TCTATAAGGCGTCTAATTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT3860.19522260940811134No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT3820.19319957718626565No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT3750.18965927079803563No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC3680.18611896440980563No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG3670.18561320635434422No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA3620.18308441607703707No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT3580.18106138385519135No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC3560.1800498677442685No Hit
GTCTCCTTCCACTCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAATATTGATAG3530.1785325935778842No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG3530.1785325935778842No Hit
ATAATATACAGCCGTGTCTTCAGTTCTCAGGCTGTTCATTTGCAGATACA3500.17701531941149992No Hit
CAGTATGATAGTTATTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCGCACGAACTGTGGCTGCACCAT3340.16892319052411706No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG3190.16133681969219563No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT3120.15779651330396566No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG3090.15627923913758135No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTAATCTATAAGGCGTCTAATTTAGAAAGTGGGGTCCCAT3090.15627923913758135No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA3050.15425620691573563No Hit
ATCATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC3030.15324469080481282No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG3030.15324469080481282No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATGATAGTTATTGGACGTTCGG3030.15324469080481282No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA3000.1517274166384285No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC2980.15071590052750566No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG2980.15071590052750566No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT2970.15021014247204423No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC2950.14919862636112136No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT2920.14768135219473708No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC2880.1456583199728914No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG2850.14414104580650708No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG2830.14312952969558423No Hit
CCTTATAGATTAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTATCAATATTCTGA2820.1426237716401228No Hit
GGGCTGGAGTGGGTGGCATTTGTCTCACATGATGGAAGAAACAAAAACTA2820.1426237716401228No Hit
GTTATTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCGCACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC2810.14211801358466136No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA2810.14211801358466136No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG2770.14009498136281565No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC2700.13655467497458565No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA2690.13604891691912424No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA2690.13604891691912424No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC2670.13503740080820137No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT2640.1335201266418171No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG2630.13301436858635568No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC2590.13099133636450994No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA2520.12745102997627994No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC2520.12745102997627994No Hit
CAATAATATACAGCCGTGTCTTCAGTTCTCAGGCTGTTCATTTGCAGATA2500.12643951386535707No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG2490.12593375580989566No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT2490.12593375580989566No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC2470.12492223969897281No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG2420.12239344942166566No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT2420.12239344942166566No Hit
GTCAGAATATTGATAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTAATCTATAAGG2380.12037041719981995No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG2330.11784162692251282No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGGCCCAGTGCATGCCAAAGTTGT2320.11733586886705138No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA2320.11733586886705138No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA2280.11531283664520567No Hit
GTATATTATTGTGTGAGAGACGATAGCAACAGCTGGTTCAATCGGCACTACTTTGACTACTGGGGCCAGGGAACC2270.11480707858974423No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC2270.11480707858974423No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC2250.11379556247882137No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAGTAGTGCCGATTGAACCAGCTGTTGCTATCGTCTCTCACACAATAATATA2240.11328980442335994No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC2220.11227828831243711No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT2210.11177253025697567No Hit
GAGTCACCATCACTTGCCGGGCCAGTCAGAATATTGATAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAG2210.11177253025697567No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC2200.11126677220151425No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT2170.10974949803512996No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA2170.10974949803512996No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC2140.10823222386874566No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGA2140.10823222386874566No Hit
GATTAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACC2120.1072207077578228No Hit
GCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACTCTGTCTGCATCTGTGGGAGA2110.10671494970236138No Hit
GCACTAGAAGTCGGCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGA2110.10671494970236138No Hit
ATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATT2100.10620919164689994No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA2090.10570343359143854No Hit
GGCATGCACTGGGCCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC2060.10418615942505424No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA2040.10317464331413138No Hit
ACCTTGATGGGACCCCACTTTCTAAATTAGACGCCTTATAGATTAGGAGCTTAGGGGCTTTCCCTGGTTTCTG2030.10266888525866996No Hit
CTTCCACTCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGG2030.10266888525866996No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT2010.1016573691477471No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG1980.10014009498136281No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTTTA250.001785178173.9509570
CCCCTAG150.002348486869.030861
GTGGTAT354.123649E-969.030861
TCTTACC200.007005127652.3827159
AATCTTA200.007019323852.3559157
CTCGGCA200.007155255452.1026650
ACGACCT200.00729313751.8518536
TTACACT200.00733708651.77314826
TGTTAGA1151.9099389E-1048.2288870
GGGGGTT305.857898E-446.6818961
GAAAGGT306.2848505E-446.02057325
GTATCAA4200.046.0205731
GGTCTCG306.2848505E-446.02057320
AGTGGTG503.814883E-641.4185185
TATCAAC4650.040.8247032
TACGGGT350.001343831539.4362315
TCAACGC4900.038.741814
ATCAACG4950.038.350483
CAACGCA5000.037.9669725
GGGCAGT557.34085E-637.6531941
Produced by FastQC (version 0.11.5)