FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197723
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10240.5178962487925026No Hit
GTATGATAGTTATTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCGCACGAACTGTGGCTGCACCATCTGTCTT8460.4278713149203684No Hit
CTCCTGTACAGCCCCTGGATTCACCCTCAACAACTTTGGCATGCACTGGGCCCGCCAGGCTCCAGGCAAGGGG7000.35403063882299984No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA6930.35049033243476985No Hit
CTATCATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC6720.33986941327007986No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6050.30598362355416414No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC5930.299914526888627No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT5900.2983972527222427No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC5750.2908108818903213No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA5520.2791784466147085No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG5450.2756381402264784No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT5360.2710863177273256No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5230.26451146300632705No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5150.26046539856263556No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC4990.25237326967525275No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG4560.23062567329041134No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC4400.2225335444030285No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT4300.2174759638484142No Hit
TCTATAAGGCGTCTAATTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT4280.21646444773749132No Hit
GAATATTGATAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTAATCTATAAGGCGT4260.21545293162656848No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG4240.2144414155156456No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT4200.2124183832937999No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT4150.20988959301649276No Hit
GTGCAGCCACAGTTCGTGCGATTTCCACCTTGGTCCCTTGGCCGAACGTC4130.2088780769055699No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT4130.2088780769055699No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC3970.20078594801818705No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAATATTGATAGCTGGTTGGCCTGGTATCAGCAGAAA3930.19876291579634134No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG3910.1977513996854185No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT3820.19319957718626565No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA3660.1851074482988828No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC3630.1835901741324985No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTGCGATTTCCACCTTGGTCC3620.18308441607703707No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT3620.18308441607703707No Hit
GCCACAGTTCGTGCGATTTCCACCTTGGTCCCTTGGCCGAACGTCCAATA3610.18257865802157563No Hit
CCTTCCACTCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCG3610.18257865802157563No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG3580.18106138385519135No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG3540.17903835163334564No Hit
CAGTATGATAGTTATTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCGCACGAACTGTGGCTGCACCAT3520.1780268355224228No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG3490.17650956135603849No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC3430.17347501302326993No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT3430.17347501302326993No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACT3320.1679116744131942No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA3230.16335985191404137No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA3220.16285409385857993No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG3210.1623483358031185No Hit
ATCATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC3200.16184257774765706No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT3160.15981954552581137No Hit
ATAATATACAGCCGTGTCTTCAGTTCTCAGGCTGTTCATTTGCAGATACA3150.15931378747034994No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATGATAGTTATTGGACGTTCGG3120.15779651330396566No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA3120.15779651330396566No Hit
GTCTCCTTCCACTCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAATATTGATAG3090.15627923913758135No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTAATCTATAAGGCGTCTAATTTAGAAAGTGGGGTCCCAT3070.1552677230266585No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG3060.15476196497119707No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT3040.15375044886027422No Hit
GGGCTGGAGTGGGTGGCATTTGTCTCACATGATGGAAGAAACAAAAACTA3020.15273893274935138No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT2880.1456583199728914No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG2800.14161225552919993No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT2800.14161225552919993No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG2780.14060073941827708No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC2770.14009498136281565No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG2720.13756619108550852No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG2660.13453164275273993No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT2610.1320028524754328No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT2550.12896830414266422No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC2530.12795678803174138No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA2510.1269452719208185No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG2480.12542799775443425No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA2460.12441648164351138No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT2440.12340496553258852No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT2440.12340496553258852No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC2430.1228992074771271No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT2430.1228992074771271No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA2410.12188769136620423No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC2380.12037041719981995No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT2370.11986465914435851No Hit
CCTTATAGATTAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTATCAATATTCTGA2370.11986465914435851No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC2320.11733586886705138No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA2300.11632435275612853No Hit
CAATAATATACAGCCGTGTCTTCAGTTCTCAGGCTGTTCATTTGCAGATA2300.11632435275612853No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC2290.11581859470066709No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC2290.11581859470066709No Hit
GTATATTATTGTGTGAGAGACGATAGCAACAGCTGGTTCAATCGGCACTACTTTGACTACTGGGGCCAGGGAACC2260.11430132053428281No Hit
GCACTAGAAGTCGGCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGA2260.11430132053428281No Hit
GTCAGAATATTGATAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTAATCTATAAGG2240.11328980442335994No Hit
CTCCTAATCTATAAGGCGTCTAATTTAGAAAGTGGGGTCCCATCAAGGTT2230.11278404636789852No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA2210.11177253025697567No Hit
GTTATTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCGCACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC2200.11126677220151425No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA2170.10974949803512996No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGGCCCAGTGCATGCCAAAGTTGT2160.10924373997966852No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA2150.1087379819242071No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA2140.10823222386874566No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC2140.10823222386874566No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2140.10823222386874566No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAGTAGTGCCGATTGAACCAGCTGTTGCTATCGTCTCTCACACAATAATATA2130.10772646581328424No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC2110.10671494970236138No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA2100.10620919164689994No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC2070.10469191748051568No Hit
ACCTTGATGGGACCCCACTTTCTAAATTAGACGCCTTATAGATTAGGAGCTTAGGGGCTTTCCCTGGTTTCTG2020.10216312720320853No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTC2020.10216312720320853No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT2000.10115161109228567No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA2000.10115161109228567No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT2000.10115161109228567No Hit
GGCATGCACTGGGCCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC1990.10064585303682425No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA1990.10064585303682425No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAAAC200.007178630752.059744
AGTGGTG405.0055372E-543.0978135
GTATCAA3850.041.2783131
TATCAAC4350.038.8572242
TCAACGC4400.037.6126374
ATCAACG4500.037.552483
CAACGCA4500.036.77685
AACGCAG4500.036.77686
CACTGGA1051.04316605E-535.7508770
CGCAGAG4750.034.841188
GTGGTAT601.3242245E-534.5481531
ACGGGGG709.710784E-734.48697316
AGTACTT807.082963E-834.47825213
TTCGGGG2002.7284841E-1134.4102170
AGAGTAC4950.033.43345311
GCAGAGT5000.033.099129
GAGTACT958.771167E-932.66360512
AAGGCTT1650.032.1295769
CAGAGTA5100.031.77407310
ACTTTTT1101.0513759E-931.35179316
Produced by FastQC (version 0.11.5)