FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1220899
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTACCTTGCATGCAGTAATAAATTCCAACATCCTCAGCCTCCACCC38990.31935483606752074No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37720.30895266520817855No Hit
GGTACACACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT37280.30534876349313084No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT37200.3046935086358495No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33710.2761080154869486No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32240.2640677074844029No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT31820.2606276194836755No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT30360.24866921833829003No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27840.22802869033392603No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27730.22712771490516417No Hit
GTACACACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA26650.21828177433186527No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA25830.211565412044731No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGACCTCCAGCAACTTCGGCACCCAGAC25250.20681481432944085No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT24120.19755933947034113No Hit
CTCCAGGCCCTTCCCTGGAACTTGCCGGACCCAATGCATGGCATGATCAT23600.19330018289801204No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23110.1892867468971635No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT22900.18756670289679983No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22740.18625619318223705No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22400.18347136003879108No Hit
AGGTACACACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT22320.1828161051815097No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA21860.17904838975214166No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20970.17175867946488613No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20610.16881003260711983No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA19570.16029171946246168No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC19320.15824404803345732No Hit
CCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGGCTCACGTCCACCACCACGCACGTGA19230.15750688631901574No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGACAGTTGAGCGCAAATGTTGTGT18870.15455823946124947No Hit
GAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTTAAAAGGTGTCCAGTGTGAAATGCAAGTGGTGGAGT18670.15292010231804598No Hit
GTATACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGAGTGGAGA18300.1498895486031195No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGATTTTC18260.1495619211744788No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18180.14890666631719743No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18170.14882475946003723No Hit
GTAATACAAGGCCGTGTCCTCAGTTTTCAGACTGTCCATTTGCAAAAACA18140.1485790388885567No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17800.14579420574511076No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG17670.1447294166020285No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCGTGGCACTCATTTA17650.14456560288770814No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17620.14431988231622764No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17520.14350081374462587No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17360.14219030403006308No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTC17280.1415350491727817No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC17230.14112551488698083No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA17200.1408797943155003No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG16910.13850449545785523No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAACATCC16860.13809496117205436No Hit
CAGTAATACAAGGCCGTGTCCTCAGTTTTCAGACTGTCCATTTGCAAAAA15670.1283480451699936No Hit
GTGTAGGTCTGGGTGCCGAAGTTGCTGGAGGTCACGGTCACCACGCTGCT15590.1276927903127122No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGAA15340.1256451188837078No Hit
CCGCTGGTCAGAGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA15190.12441651602630521No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG15090.12359744745470347No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA14980.12269647202594154No Hit
CTCCTGTGTAGCCTCTGGATTCACCTTTCATGATCATGCCATGCATTGGGTCCGGCAAGTTCCAGGGAAGGGC14890.12195931031149998No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGGCAG14810.12130405545421857No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14800.12122214859705839No Hit
GGCCTGGCCTCTGCTGAAACCAATTCAAGTAGGTGTTTCCATCACTGTAT14270.11688108516756915No Hit
GTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA14200.11630773716744791No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14010.1147515068814046No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13980.11450578630992408No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGA13820.11319527659536129No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13690.11213048745227902No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13590.11131141888067726No Hit
GTCTGGGACCCCAGAGTCCCAATTAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA13550.11098379145203657No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTT13540.1109018845948764No Hit
CTCGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCC13400.10975518859463396No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA13280.10877230630871185No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13250.10852658573723133No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13050.10688844859402785No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT13020.10664272802254733No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13020.10664272802254733No Hit
CTATTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGTCTTCCCCCTGGCGCC13020.10664272802254733No Hit
GGCCTGGAGTGGGTCTCAGGTATTTTTTGGAATAGTGGTGGGGTAGGCTA13000.10647891430822698No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTATA12920.10582365945094557No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12770.10459505659354296No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTT12710.10410361545058192No Hit
GTGCTGGGCACGGTGGGCACTCGACACAACATTTGCGCTCAACTGTCTTG12700.10402170859342173No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12560.1028750125931793No Hit
CAACTGTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATCTACGTTGCAGGTGTAGGTCTGGGTGCCGAAGTTG12500.10238357145021823No Hit
CGTCCACCACCACGCACGTGACCTCAGGGGTCCGGGAGATCATGAGGGTG12350.10115496859281561No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGAC200.007404555651.66953721
CTTATAC1850.042.8181881
TATACAC2050.042.0008743
TCGCTAA1900.041.74096731
CGTATGC1950.040.7810641
TCGTATG1950.040.76429740
GTATCAA14050.040.691521
CGCTAAT2000.039.65880232
GTGGTAT1850.039.0948681
GGTATCA5900.038.52691
TTATACA2150.038.445452
GCTAATC2000.036.2161533
TGGTATC2000.036.162752
CCTTTGT13900.035.6354870
AATCTCG2100.034.50997536
TATCAAC16650.034.3372882
ATCAACG16850.033.725333
TCAACGC16750.033.7210584
CTAATCT2150.033.6949734
ACACTCG2400.033.0205627
Produced by FastQC (version 0.11.5)