FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1220899
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTACCTTGCATGCAGTAATAAATTCCAACATCCTCAGCCTCCACCC41350.33868485435732193No Hit
GGTACACACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT36620.29994291092055936No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36090.2956018474910701No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT35190.2882302303466544No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32940.2698011874856151No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT32020.26226575662687907No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29700.24326336576571853No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27730.22712771490516417No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGACCTCCAGCAACTTCGGCACCCAGAC27070.22172186233259264No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT27000.2211485143324714No Hit
GTACACACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA26850.21991991147506879No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25590.20959964747288679No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA22870.1873209823253193No Hit
CTCCAGGCCCTTCCCTGGAACTTGCCGGACCCAATGCATGGCATGATCAT22580.18494568346767423No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22480.1841266148960725No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22420.18363517375311142No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22120.18117796803830621No Hit
AGGTACACACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT22090.18093224746682568No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT20830.17061198346464368No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT20580.16856431203563932No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA20370.16684426803527563No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA19920.16315845946306778No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19280.15791642060481662No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA19000.15562302860433172No Hit
GTAATACAAGGCCGTGTCCTCAGTTTTCAGACTGTCCATTTGCAAAAACA18970.1553773080328512No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC18060.14792378403127532No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18050.14784187717411515No Hit
GTCTGGGACCCCAGAGTCCCAATTAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA17900.14661327431671253No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC17880.14644946060239217No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGACAGTTGAGCGCAAATGTTGTGT17870.146367553745232No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17860.14628564688807183No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17460.14300937260166485No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTC17450.14292746574450466No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17170.14063407374401976No Hit
GTATACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGAGTGGAGA16980.13907784345797647No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16900.13842258860069506No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16760.13727589260045261No Hit
GAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTTAAAAGGTGTCCAGTGTGAAATGCAAGTGGTGGAGT16720.1369482651718119No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG16570.1357196623144093No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGGCAG16300.1335081771710846No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16250.1330986428852837No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGATTTTC16120.13203385374220145No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16050.1314605057420802No Hit
CCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGGCTCACGTCCACCACCACGCACGTGA16000.13105097145627936No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15980.130887157741959No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGAA15930.13047762345615813No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15890.13014999602751742No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAACATCC15820.1295766480273962No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCGTGGCACTCATTTA15510.1270375354554308No Hit
CAGTAATACAAGGCCGTGTCCTCAGTTTTCAGACTGTCCATTTGCAAAAA15420.12630037374098924No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTT15190.12441651602630521No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14910.12212312402582032No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG14840.1215497760256991No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA14710.12048498688261682No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT14640.1199116388824956No Hit
GTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA14530.11901066345373368No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14120.11565248231016653No Hit
CTCCTGTGTAGCCTCTGGATTCACCTTTCATGATCATGCCATGCATTGGGTCCGGCAAGTTCCAGGGAAGGGC13940.1141781588812834No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG13930.11409625202412321No Hit
GGCCTGGCCTCTGCTGAAACCAATTCAAGTAGGTGTTTCCATCACTGTAT13840.11335909030968164No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA13230.10836277202291099No Hit
GTCTGAAAACTGAGGACACGGCCTTGTATTACTGTGTAAAAGATGTCTTA13210.10819895830859064No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTT13210.10819895830859064No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12790.1047588703078633No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12560.1028750125931793No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12550.10279310573601912No Hit
CTATTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGTCTTCCCCCTGGCGCC12520.1025473851645386No Hit
GGCCAAAAGTGTACGGCCAGTGTGTACCTTGCATGCAGTAATAAATTCCA12460.10205594402157754No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12450.10197403716441736No Hit
ATACAAGGCCGTGTCCTCAGTTTTCAGACTGTCCATTTGCAAAAACACGG12450.10197403716441736No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12360.10123687544997581No Hit
GCCTTGTATTACTGTGTAAAAGATGTCTTACCCGGCGGTGCGGACTATTGGGGCCAGGGAACCCAGGTCACCGT12310.10082734116417492No Hit
GTTCAACAGCACGTTCCGTGTGGTCAGCGTCCTCACCGTCGTGCACCAGG12250.10033590002121388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13700.039.7773131
GTGGTAT2150.038.5009731
CCTTTGT13700.038.1603270
GGTATCA6150.034.210041
TATCAAC16000.033.585452
TCAACGC16250.033.0308274
AACGCAG16500.032.9460646
CAACGCA16300.032.9281545
ATCAACG16350.032.8409083
ATTCGCT450.00411072231.4113264
AGAGTAC17550.031.17097511
ATAGGAC450.004275783431.15966458
ACGCAGA17550.030.974937
CGCAGAG17900.030.3692788
ATCAGTG18750.030.20601370
CAGAGTA18300.029.89347810
TGGTATC2550.029.7186472
TGTTAGA9650.028.37779670
GCAGAGT19850.027.7325559
TTCGGGG11450.027.72157970
Produced by FastQC (version 0.11.5)