FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences689986
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21900.041.9960631
GGTATCA8350.040.8404161
TATCAAC24650.036.7119372
ATCAACG25100.035.888023
TCAACGC25250.035.656734
CAACGCA25500.035.441915
AACGCAG26400.034.4939966
ACGCAGA27750.032.815917
AGAGTAC28750.031.67448611
CGCAGAG28900.031.5100868
CAGAGTA30000.030.58380910
GCAGAGT30250.030.1038519
GTATAGA1005.08162E-727.5569691
TAATACG500.00788347927.4910644
AGTACGG23900.027.32050513
GAGTACG23900.027.31852512
TGGTATC3050.027.075712
GTGGTAT3300.026.0956131
CGGGTAC1208.821371E-825.77847717
GTATTAG2950.024.521031
Produced by FastQC (version 0.11.5)