FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505565
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32360.6400759546250235No Hit
GTATACGGGCCCCGTACGCATTTGGTCAGGGGACCAAGCTGGAGATCAAA25620.5067597638285878No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22920.4533541681089474No Hit
GTGATGGAGAGACGTATTTGTATTGGTACCTACAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTATGAACTTT22030.43575010137173265No Hit
GTGCAGATATTGTGCTGACCCAGACTCCACGCTCTCTGTCCGTCACCCCT21090.4171570421211911No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19920.39401461730934695No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19770.3910476397693669No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19290.38155331164143086No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18860.3730479760268215No Hit
CCCCTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG17350.3431804021243559No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16420.3247851413764798No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16200.3204335743178424No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTGATGGAGAGACGTATTTGTATTGGTACCTACAGAAGCCAGGCCAGCCTC15770.31192823870323305No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15110.29887353752732093No Hit
GGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG14790.2925439854420302No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14190.28067607528211014No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGACCAAATGCG14130.27948928426611813No Hit
GTTCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGTCAGAGACAGATT13580.2686103666195247No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTTCTCAGTTCATCTTCTCACC13540.26781917260886334No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13490.26683018009553666No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATGCAGTGCAGATATTGTGCTGA13120.25951163549691925No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12870.2545666729302859No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12700.2512040983849752No Hit
GGATACCTGGATGCAGTGCAGATATTGTGCTGACCCAGACTCCACGCTCTCTGTCCGTCACCCCTGGACAGCCGG12560.24843491934766052No Hit
GACCCAGACTCCACGCTCTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCT12480.24685253132633783No Hit
CATTTGGTCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA12110.23953398672772047No Hit
GATCAGGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATA11980.2369626061930711No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11240.22232551699583633No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11190.22133652448250965No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC11060.21876514394786034No Hit
CTCCTGCATAGTGATGGAGAGACGTATTTGTATTGGTACCTACAGAAGCCAGGCCAGCCTCCACAGCTCCTGAT11040.21836954694252966No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11020.21797394993719899No Hit
CCTGGATGCAGTGCAGATATTGTGCTGACCCAGACTCCACGCTCTCTGTC10990.21738055442920298No Hit
GAAAGTATACGGGCCCCGTACGCATTTGGTCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT10970.2169849574238723No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10900.21560036790521497No Hit
AGCCTCCACAGCTCCTGATCTATGAACTTTCCAGCCGGTTCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGT10700.21164439785190825No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10610.20986421132792024No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10600.2096664128252549No Hit
GCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATGCAGTGCAGATATTGTGCTGACCCAGACTCCACGCTCT10530.20828182330659756No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCGACTGATTGTCAGTGTGAAATCTGTCTCTGACCCGCTGCCA10480.20729283079327088No Hit
GTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT10440.20650163678260952No Hit
GACCAAATGCGTACGGGGCCCGTATACTTTCCATGCAGTAATAAACCCCA10350.20472145025862157No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATGCAGTGCAGATATTGTGCTGACCCAGACTCCACG10170.20116107721064552No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9850.19483152512535482No Hit
GCTAATGCTCTGGATACCTGGATGCAGTGCAGATATTGTGCTGACCCAGACTCCACGCTCTCTGTCCGTCACCC9650.1908755550720481No Hit
CTACAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTATGAACTTTCCAG9570.18929316705072544No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9570.18929316705072544No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9030.17861204790679736No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8990.177820853896136No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8750.17307368983216798No Hit
GTATTGGTACCTACAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTATG8610.1703045107948533No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8560.1693155182815266No Hit
GCATAGTGATGGAGAGACGTATTTGTATTGGTACCTACAGAAGCCAGGCC8530.1687221227735306No Hit
CCCCTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCATAGTGATGGAGAGACGTATTTGT8480.16773313026020392No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8320.16456835421755855No Hit
GTTCAGTGGCAGCGGGTCAGAGACAGATTTCACACTGACAATCAGTCGGG8180.1617991751802439No Hit
GTGGAGGCTGGCCTGGCTTCTGTAGGTACCAATACAAATACGTCTCTCCA8140.16100798116958254No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8080.15982119015359053No Hit
GTTTATTACTGCATGGAAAGTATACGGGCCCCGTACGCATTTGGTCAGGGGACCAAGCTGGAGATCAAACGAA7980.15784320512693717No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7940.15705201111627584No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC7920.15665641411094516No Hit
GTATACTTTCCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCCGACTGATTGTCAGTGTGAAAT7840.1550740260896225No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7810.1544806305816265No Hit
GGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGGAAAGTATACGGGCCCCGTACGCATTTGGTCAGGGGAC7720.1527004440576385No Hit
TCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGTCAGAGACAGATTTCACACTGACAATCAGTCGGGTGGAGG7700.15230484705230782No Hit
CCAGAGAACCGGCTGGAAAGTTCATAGATCAGGAGCTGTGGAGGCTGGCC7580.14993126502032378No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7560.14953566801499313No Hit
GCTCCTGATCTATGAACTTTCCAGCCGGTTCTCTGGAGTGCCAGATAGGT7510.14854667550166645No Hit
CTCTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA7500.14834887699900112No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7490.1481510784963358No Hit
CTCCATCACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC7410.1465686904750131No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7400.14637089197234776No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7330.14498630245369043No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG7270.1437995114376984No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATGCAGTGCAGATATTGTGCTGACCCAGACTC7240.1432061159297024No Hit
ACAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTATGAACTTTCCAGCCGGTTCTCTGGAGTGCCAGATAGGTT7170.14182152641104506No Hit
AGTCAGAGCCTCCTGCATAGTGATGGAGAGACGTATTTGTATTGGTACCTACAGAAGCCAGGCCAGCCTCCACAG7070.13984354138439173No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC7060.13964574288172638No Hit
CTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGT7040.13925014587639573No Hit
CCTGCATAGTGATGGAGAGACGTATTTGTATTGGTACCTACAGAAGCCAG7010.1386567503683997No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6930.137074362347077No Hit
GTGCCAGATAGGTTCAGTGGCAGCGGGTCAGAGACAGATTTCACACTGAC6930.137074362347077No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT6930.137074362347077No Hit
GATTTCACACTGACAATCAGTCGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGGAAAGTATACGGGCC6850.13549197432575436No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT6750.13351398929910102No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC6670.1319316012777783No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT6600.13054701175912098No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT6590.13034921325645565No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG6550.12955801924579433No Hit
GCCCGTATACTTTCCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCCGACTGATTGTCAGTGTGAAAT6480.12817342972713697No Hit
TCTATGAACTTTCCAGCCGGTTCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGTCAGAGACAGATTTCACA6470.12797563122447164No Hit
ATATTGTGCTGACCCAGACTCCACGCTCTCTGTCCGTCACCCCTGGACAG6460.1277778327218063No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT6420.12698663871114496No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG6340.12540425068982228No Hit
CTCCAGAGAACCGGCTGGAAAGTTCATAGATCAGGAGCTGTGGAGGCTGG6320.1250086536844916No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT6310.12481085518182627No Hit
GTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGA6270.12401966117116493No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6190.12243727314984226No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA6070.12006369111785824No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC6040.11947029560986223No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA6010.11887690010186623No Hit
ATCTGGCACTCCAGAGAACCGGCTGGAAAGTTCATAGATCAGGAGCTGTGGAGGCTGGCCTGGCTTCTGTAGGTA6000.11867910159920089No Hit
GGTCAGAGACAGATTTCACACTGACAATCAGTCGGGTGGAGGCTGAGGAT5930.11729451208054355No Hit
TCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTG5930.11729451208054355No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC5900.11670111657254753No Hit
GATCAGGAGCTGTGGAGGCTGGCCTGGCTTCTGTAGGTACCAATACAAAT5880.11630551956721688No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC5830.11531652705389021No Hit
GTATTTGTATTGGTACCTACAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTATGAACTTTCCAGCCGGTTCT5760.11393193753523286No Hit
GGTATCAACGCAGAGTACGGGGATCAGGACTTCTCAGTTCATCTTCTCAC5740.11353634052990219No Hit
GACAATCAGTCGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGG5720.11314074352457151No Hit
GATAGGTTCAGTGGCAGCGGGTCAGAGACAGATTTCACACTGACAATCAG5680.11234954951391017No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG5640.11155835550324883No Hit
CTCCACGCTCTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGC5630.1113605570005835No Hit
CCCGTATACTTTCCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCCGACTGATTGTCAGTGTGAAAT5570.11017376598459148No Hit
AGCCAGGCCAGCCTCCACAGCTCCTGATCTATGAACTTTCCAGCCGGTTC5560.10997596748192615No Hit
GTACGCATTTGGTCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG5540.1095803704765955No Hit
TCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT5530.10938257197393017No Hit
ATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAGGGGTGACGGACAGAGAGCGTGGAGT5460.10799798245527281No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC5420.10720678844461148No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC5400.1068111914392808No Hit
CCACAGCTCCTGATCTATGAACTTTCCAGCCGGTTCTCTGGAGTGCCAGA5390.10661339293661547No Hit
GTTCATAGATCAGGAGCTGTGGAGGCTGGCCTGGCTTCTGTAGGTACCAA5360.10601999742861946No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA5340.1056244004232888No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT5330.10542660192062346No Hit
GATATTGTGCTGACCCAGACTCCACGCTCTCTGTCCGTCACCCCTGGACA5310.10503100491529278No Hit
GTCGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGGAAAGTATA5310.10503100491529278No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA5230.1034486168939701No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC5200.10285522138597412No Hit
CCCAGACTCCACGCTCTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTC5200.10285522138597412No Hit
GGTTTATTACTGCATGGAAAGTATACGGGCCCCGTACGCATTTGGTCAGGGGACCAAGCTGGAGATCAAACGAA5170.10226182587797811No Hit
CACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATGCAGTGCAGATATTGTG5150.10186622887264744No Hit
GTGCTGACCCAGACTCCACGCTCTCTGTCCGTCACCCCTGGACAGCCGGC5150.10186622887264744No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT5100.10087723635932076No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)