FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505565
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33830.6691523345168278No Hit
GTATACGGGCCCCGTACGCATTTGGTCAGGGGACCAAGCTGGAGATCAAA25270.4998368162353011No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20150.39856398287064965No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20020.3959926023360003No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19190.3795753266147775No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18290.3617734613748974No Hit
GTGATGGAGAGACGTATTTGTATTGGTACCTACAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTATGAACTTT17110.3384332380603879No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16840.33309267848842383No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCGACTGATTGTCAGTGTGAAATCTGTCTCTGACCCGCTGCCA16830.3328948799857585No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16550.32735652191112913No Hit
GTGCAGATATTGTGCTGACCCAGACTCCACGCTCTCTGTCCGTCACCCCT16240.3212247683285038No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16060.3176643952805277No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGACCAAATGCG15870.31390622372988636No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13220.2614896205235726No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTTCTCAGTTCATCTTCTCACC13050.25812704597826197No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12680.25080850137964456No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTGATGGAGAGACGTATTTGTATTGGTACCTACAGAAGCCAGGCCAGCCTC12570.24863271785032587No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12480.24685253132633783No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12260.24250096426770051No Hit
GGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG12210.24151197175437383No Hit
GACCAAATGCGTACGGGGCCCGTATACTTTCCATGCAGTAATAAACCCCA12180.24091857624637783No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC11740.2322154421291031No Hit
GAAAGTATACGGGCCCCGTACGCATTTGGTCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT11680.23102865111311108No Hit
CATTTGGTCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA11640.23023745710244975No Hit
GTATACTTTCCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCCGACTGATTGTCAGTGTGAAAT11560.22865506908112707No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11470.22687488255713906No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11370.2248968975304857No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11170.22094092747717897No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11010.21777615143453366No Hit
GTTCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGTCAGAGACAGATT10980.21718275592653766No Hit
GATCAGGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATA10940.2163915619158763No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10490.2074906292959362No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10220.2021500697239722No Hit
CCCCTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG10000.19779850266533483No Hit
GGATACCTGGATGCAGTGCAGATATTGTGCTGACCCAGACTCCACGCTCTCTGTCCGTCACCCCTGGACAGCCGG9970.1972051071573388No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATGCAGTGCAGATATTGTGCTGA9820.1942381296173588No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9540.18869977154272943No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9480.18751298052673743No Hit
GCCCGTATACTTTCCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCCGACTGATTGTCAGTGTGAAAT9460.18711738352140675No Hit
GTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT9140.18078783143611604No Hit
AGCCTCCACAGCTCCTGATCTATGAACTTTCCAGCCGGTTCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGT9100.1799966374254547No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9070.17940324191745868No Hit
GACCCAGACTCCACGCTCTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCT9000.17801865239880132No Hit
CTCCTGCATAGTGATGGAGAGACGTATTTGTATTGGTACCTACAGAAGCCAGGCCAGCCTCCACAGCTCCTGAT8620.1705023092975186No Hit
CCCCTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCATAGTGATGGAGAGACGTATTTGT8540.16891992127619596No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8460.16733753325487327No Hit
GGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGGAAAGTATACGGGCCCCGTACGCATTTGGTCAGGGGAC8450.16713973475220792No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8440.1669419362495426No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8400.16615074223888124No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8380.1657551452335506No Hit
GCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATGCAGTGCAGATATTGTGCTGACCCAGACTCCACGCTCT8340.16496395122288923No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8330.1647661527202239No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8090.16001898865625588No Hit
CCCGTATACTTTCCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCCGACTGATTGTCAGTGTGAAAT8080.15982119015359053No Hit
GTTTATTACTGCATGGAAAGTATACGGGCCCCGTACGCATTTGGTCAGGGGACCAAGCTGGAGATCAAACGAA8050.15922779464559453No Hit
CCTGGATGCAGTGCAGATATTGTGCTGACCCAGACTCCACGCTCTCTGTC7890.1560630186029492No Hit
GTGTGAAATCTGTCTCTGACCCGCTGCCACTGAACCTATCTGGCACTCCA7790.15408503357629583No Hit
GTTCAGTGGCAGCGGGTCAGAGACAGATTTCACACTGACAATCAGTCGGG7760.15349163806829982No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATGCAGTGCAGATATTGTGCTGACCCAGACTCCACG7760.15349163806829982No Hit
GTATTGGTACCTACAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTATG7540.14914007100966248No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7460.1475576829883398No Hit
CTCCATCACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC7270.1437995114376984No Hit
GCTAATGCTCTGGATACCTGGATGCAGTGCAGATATTGTGCTGACCCAGACTCCACGCTCTCTGTCCGTCACCC7260.14360171293503307No Hit
CCAGAGAACCGGCTGGAAAGTTCATAGATCAGGAGCTGTGGAGGCTGGCC7170.14182152641104506No Hit
CTACAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTATGAACTTTCCAG7140.14122813090304906No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7040.13925014587639573No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7000.13845895186573437No Hit
TCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGTCAGAGACAGATTTCACACTGACAATCAGTCGGGTGGAGG6990.13826115336306904No Hit
GTGGAGGCTGGCCTGGCTTCTGTAGGTACCAATACAAATACGTCTCTCCA6960.13766775785507304No Hit
GATTTCACACTGACAATCAGTCGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGGAAAGTATACGGGCC6940.13727216084974236No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC6850.13549197432575436No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC6800.13450298181242767No Hit
GCATAGTGATGGAGAGACGTATTTGTATTGGTACCTACAGAAGCCAGGCC6800.13450298181242767No Hit
GCCTCCACCCGACTGATTGTCAGTGTGAAATCTGTCTCTGACCCGCTGCC6740.13331619079643567No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT6710.13272279528843967No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA6670.1319316012777783No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6580.1301514147537903No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC6520.12896462373779832No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6500.12856902673246765No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6460.1277778327218063No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT6460.1277778327218063No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA6430.1271844372138103No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG6360.12579984769515296No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC6310.12481085518182627No Hit
GCTCCTGATCTATGAACTTTCCAGCCGGTTCTCTGGAGTGCCAGATAGGT6300.12461305667916094No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6300.12461305667916094No Hit
GTCTCTGACCCGCTGCCACTGAACCTATCTGGCACTCCAGAGAACCGGCT6290.1244152581764956No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA6260.1238218626684996No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT6210.12283287015517294No Hit
CTCCAGAGAACCGGCTGGAAAGTTCATAGATCAGGAGCTGTGGAGGCTGG6210.12283287015517294No Hit
CTCTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA6200.1226350716525076No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT6180.12223947464717692No Hit
ATCTGGCACTCCAGAGAACCGGCTGGAAAGTTCATAGATCAGGAGCTGTGGAGGCTGGCCTGGCTTCTGTAGGTA6170.1220416761445116No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA6160.12184387764184626No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC6140.12144828063651558No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC6140.12144828063651558No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA6130.12125048213385026No Hit
ACAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTATGAACTTTCCAGCCGGTTCTCTGGAGTGCCAGATAGGTT6080.12026148962052358No Hit
TCTATGAACTTTCCAGCCGGTTCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGTCAGAGACAGATTTCACA5990.11848130309653555No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC5960.11788790758853955No Hit
GTGCCAGATAGGTTCAGTGGCAGCGGGTCAGAGACAGATTTCACACTGAC5940.11749231058320887No Hit
TCCTCAGCCTCCACCCGACTGATTGTCAGTGTGAAATCTGTCTCTGACCCGCTGCCACTGAACCTATCTGGCACT5780.11432753454056353No Hit
GACAATCAGTCGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGG5760.11393193753523286No Hit
GGTCAGAGACAGATTTCACACTGACAATCAGTCGGGTGGAGGCTGAGGAT5750.11373413903256753No Hit
CCTGCATAGTGATGGAGAGACGTATTTGTATTGGTACCTACAGAAGCCAG5750.11373413903256753No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC5750.11373413903256753No Hit
GATCAGGAGCTGTGGAGGCTGGCCTGGCTTCTGTAGGTACCAATACAAAT5680.11234954951391017No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC5660.11195395250857951No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATGCAGTGCAGATATTGTGCTGACCCAGACTC5650.11175615400591417No Hit
AGTCAGAGCCTCCTGCATAGTGATGGAGAGACGTATTTGTATTGGTACCTACAGAAGCCAGGCCAGCCTCCACAG5610.11096495999525283No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC5580.11037156448725682No Hit
GTACGCATTTGGTCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG5550.10977816897926082No Hit
GGTTTATTACTGCATGGAAAGTATACGGGCCCCGTACGCATTTGGTCAGGGGACCAAGCTGGAGATCAAACGAA5540.1095803704765955No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT5490.10859137796326883No Hit
ATATTGTGCTGACCCAGACTCCACGCTCTCTGTCCGTCACCCCTGGACAG5480.10839357946060349No Hit
CTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGT5450.10780018395260749No Hit
GTTCATAGATCAGGAGCTGTGGAGGCTGGCCTGGCTTCTGTAGGTACCAA5450.10780018395260749No Hit
GGCTGAGGATGTTGGGGTTTATTACTGCATGGAAAGTATACGGGCCCCGTACGCATTTGGTCAGGGGACCAAG5380.10641559443395013No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG5350.10582219892595413No Hit
TCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTG5270.10423981090463144No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA5240.10364641539663544No Hit
GTATTTGTATTGGTACCTACAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTATGAACTTTCCAGCCGGTTCT5150.10186622887264744No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGAC5130.10147063186731677No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACCCGACTGATTGTCAGTGTGAAATCTGTCTCTGACCCGCTGCCA5060.10008604234865942No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACACC252.6089506E-455.013974
ATAAACG200.007446764551.5909043
TAAACGC200.007455508751.5755924
ACGATCA200.00745842551.57049613
GGTATCA3950.046.244911
CCTTATA306.339204E-445.954061
TGGTATC1050.045.9085462
GTATCAA9850.041.6385771
TGTTAGA4500.041.50082470
GTGGTAT1250.041.3586581
GTGTATA400.002607704134.4655461
TATCAAC12000.034.4314082
AGCAAAT400.002634441234.39393618
ACGGGAA400.0026357234.39053316
AATTTAA750.002650524734.3455170
ATCAACG12550.033.434743
CAACGCA12800.032.7687535
TCAACGC12850.032.6444744
AACGCAG13200.032.557136
TATAAGA652.3731007E-531.7388294
Produced by FastQC (version 0.11.5)