FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666848
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10630.15940664139354094No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGATA306.306428E-446.0045735
GGTATCA8550.038.239181
GTATCAA18000.037.47441
GTGGTAT3050.033.851081
TCAACGC21100.032.5550464
CAACGCA21300.032.403325
TATCAAC21300.032.2905352
ATCAACG21250.032.0139963
AACGCAG23100.030.324336
CGCAGAG23750.029.4944048
ACGCAGA23900.029.309297
AGAGTAC24650.028.13892611
CAGAGTA25200.027.52478210
CGTGACG550.00832578627.18468968
ACGGGTA1057.997569E-726.16216916
TGGTATC3750.025.6774332
GCAGAGT27200.025.5008989
TCTGTCG951.08897675E-525.3015698
GAGTACG18650.025.22404512
AGTACGG18650.025.22404513
Produced by FastQC (version 0.11.5)