FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences939723
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28850.039.7338521
GGTATCA12950.038.0126841
TATCAAC33200.034.5668682
ATCAACG33750.033.7729573
TCAACGC33800.033.7122234
CAACGCA34800.032.8368645
AACGCAG35100.032.2629136
GCGTATA553.3352192E-431.2362582
ACGCAGA36600.030.84697
AGAGTAC37800.029.68606811
CAGAGTA38300.029.47771810
CGCAGAG39100.028.9623578
GCAGAGT40750.027.7054399
TACCGTC500.0079386727.4528037
CCGCTAT1606.7641377E-626.90047370
TCGTCAA1606.7641377E-626.90047370
ACCGTCG658.9304324E-426.3969298
AGTACGG32000.026.2731913
GAGTACG33400.024.96643412
GTGGTAT4300.024.8173561
Produced by FastQC (version 0.11.5)