FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences627112
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19260.30712217275383025No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA11430.18226409317633852No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11240.1792343313475105No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10950.17460995802982562No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10880.1734937299876258No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10700.17062342930768348No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10620.16934774011659798No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCCGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGGGG8570.13665820459503245No Hit
GTTTATTACTGTCAGCACCGTAGCGACTGGTCCGCGCTCACTTTCGGCGG8180.13043921978849074No Hit
GCCTAGAACCTGAAGATTTTGCAGTTTATTACTGTCAGCACCGTAGCGAC8070.1286851471507482No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8070.1286851471507482No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8030.12804730255520544No Hit
GTCTTTGTCTCCCGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGGGGCTACTTAGCCTGGTA7990.12740945795966271No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGGGGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT7460.11895801706872138No Hit
GTGTTAGGGGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC7350.11720394443097884No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7340.11704448328209315No Hit
GTGCAGCCACAGTTCGTTTGATCTGCACCTTGGTCCCTCCGCCGAAAGTGAGCGCGGACCAGTCGCTACGGTG7310.1165660998354361No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7310.1165660998354361No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7130.11369579915549376No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7110.11337687685772238No Hit
GTCTGGGACGGACTTCAGTCTCACCATCAGCAGCCTAGAACCTGAAGATT7030.11210118766663689No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7010.11178226536886554No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6830.1089119646889232No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6820.10875250354003752No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT6790.10827412009338044No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6700.10683896975340929No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA6470.1031713633290385No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)