FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences886952
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCCACTCTGTGTCGGGGTCTCCGGGGGAGACGATAACCATTTCCTGCACCCGCACCAGTGGCGGCATTGCCAG58610.660802388404333No Hit
GCCTTGGGCTGACCCAGGACGGTCAGTTTGGTCCCTCCGCCGAACACTTT57200.6449052485365612No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC55100.621228657244135No Hit
GTGTCGGGGTCTCCGGGGGAGACGATAACCATTTCCTGCACCCGCACCAGTGGCGGCATTGCCAGCGCCTTTGTT39470.44500717062479145No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT36230.4084775726307624No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC34440.3882960971957896No Hit
TCTCAGGCCTGAAGACCGATGACGAGGCTGACTACTATTGTCAGTCTCAT34130.38480098133833623No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31150.3512027708376552No Hit
CCACTAAAGTGTTCGGCGGAGGGACCAAACTGACCGTCCTGGGTCAGCCC27760.3129819877513101No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC27570.31083981996770965No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGTTTGGTCCCTC27550.3106143286220675No Hit
GAGTGGGGCTGAGTCAGCATAAAATTGGCCCAAGAACCTGTGCAGTGAGCGAGGAGGGTGAGAAGTAGTGGAGCC27160.3062172473820455No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25740.2902073618414525No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG25410.286486754638357No Hit
GGCCTGAGATGGTCAGGGAGGCAGAGTTGGAGGAGGCGTCGATGGAGCCA25130.28332987579936686No Hit
CTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCGGGGTCTCCGGGGGAGACGATAAC24770.279271031577808No Hit
CTTTAGTGGTGCTATCATGAGACTGACAATAGTAGTCAGCCTCGTCATCG24670.2781435748495973No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG24540.27667788110292324No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT22540.2541287465387078No Hit
GACCCAGGACGGTCAGTTTGGTCCCTCCGCCGAACACTTTAGTGGTGCTATCATGAGACTGACAATAGTAGTCAG22300.251422850391002No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22140.24961891962586474No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20800.2345109994678404No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20550.2316923576473135No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20500.23112862928320813No Hit
CCCCGACACAGAGTGGGGCTGAGTCAGCATAAAATTGGCCCAAGAACCTG20460.2306776465919238No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG20270.22853547880832334No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20030.2258295826606175No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19600.22098151872931115No Hit
GAGTTGGAGGAGGCGTCGATGGAGCCAGAGAATCGATCAGGGACCCCAGA19540.2203050446923847No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19480.21962857065545824No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19090.21523148941543624No Hit
GTGGTACCAGCAGCGCCCGGGCAGTTCCCCCACCGGTGTGATTTATGAGGATAATAAAAGACCCTCTGGGGTCC18970.2138785413415833No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG18460.20812851202770838No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18460.20812851202770838No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18420.20767752933642408No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17950.20237848271383346No Hit
CCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGA17560.19798140147381144No Hit
CCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTT17350.1956137423445688No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA17080.19256960917839971No Hit
GTTCCCCCACCGGTGTGATTTATGAGGATAATAAAAGACCCTCTGGGGTC16900.19054018706762033No Hit
ACCCAGGACGGTCAGTTTGGTCCCTCCGCCGAACACTTTAGTGGTGCTATCATGAGACTGACAATAGTAGTCAGC16840.18986371303069388No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16800.18941273033940956No Hit
GCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCT16700.1882852736111988No Hit
GTGGGGGAACTGCCCGGGCGCTGCTGGTACCACTGAACAAAGGCGCTGGC16570.1868195798645248No Hit
CTACTATTGTCAGTCTCATGATAGCACCACTAAAGTGTTCGGCGGAGGGACCAAACTGACCGTCCTGGGTCAGCC16570.1868195798645248No Hit
TCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATG16300.18377544669835572No Hit
CAATAGTAGTCAGCCTCGTCATCGGTCTTCAGGCCTGAGATGGTCAGGGA16270.1834372096798925No Hit
TGATAGCACCACTAAAGTGTTCGGCGGAGGGACCAAACTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGT15850.17870189142140724No Hit
CTGCTGGTACCACTGAACAAAGGCGCTGGCAATGCCGCCACTGGTGCGGGTGCAGGAAATGGTTATCGTCTCCCC15760.17768718036601755No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15600.1758832496008803No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15430.17396657316292202No Hit
GTGCTATCATGAGACTGACAATAGTAGTCAGCCTCGTCATCGGTCTTCAG15350.1730646077803534No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15320.17272637076189015No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGT15290.17238813374342693No Hit
GGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCC15270.17216264239778478No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15250.17193715105214263No Hit
CTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCT15210.1714861683608583No Hit
GTTTGGTCCCTCCGCCGAACACTTTAGTGGTGCTATCATGAGACTGACAA14960.16866752654033138No Hit
ATAAAAGACCCTCTGGGGTCCCTGATCGATTCTCTGGCTCCATCGACGCC14930.16832928952186815No Hit
CTCCGGGGGAGACGATAACCATTTCCTGCACCCGCACCAGTGGCGGCATT14860.1675400698121206No Hit
GTCTTCAGGCCTGAGATGGTCAGGGAGGCAGAGTTGGAGGAGGCGTCGATGGAGCCAGAGAATCGATCAGGGACC14830.16720183279365738No Hit
ATCATGAGACTGACAATAGTAGTCAGCCTCGTCATCGGTCTTCAGGCCTG14710.16584888471980447No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14690.16562339337416232No Hit
CTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACT14520.16370671693620398No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT14510.16359397126338293No Hit
ATTTTATGCTGACTCAGCCCCACTCTGTGTCGGGGTCTCCGGGGGAGACG14320.16145180347978244No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14190.15998610973310842No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGTTTGGTCCCTCCGCCGAACACTTTAGTGGTG14160.1596478727146452No Hit
CTCCAACTCTGCCTCCCTGACCATCTCAGGCCTGAAGACCGATGACGAGG13890.15660373954847612No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13790.15547628282026535No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13700.15446157176487565No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13540.15265764099973844No Hit
ATAGTAGTCAGCCTCGTCATCGGTCTTCAGGCCTGAGATGGTCAGGGAGG13520.15243214965409627No Hit
GACTACTATTGTCAGTCTCATGATAGCACCACTAAAGTGTTCGGCGGAGGGACCAAACTGACCGTCCTGGGTCAG13370.1507409645617801No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG13350.15051547321613795No Hit
GCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCG13240.1492752708151061No Hit
ACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACT13050.14713310303150565No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC12870.14510368092072626No Hit
GAACAAAGGCGCTGGCAATGCCGCCACTGGTGCGGGTGCAGGAAATGGTTATCGTCTCCCCCGGAGACCCCGACA12800.14431446121097874No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12760.14386347851969442No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12610.14217229342737825No Hit
ACTCTGTGTCGGGGTCTCCGGGGGAGACGATAACCATTTCCTGCACCCGC12280.13845168622428272No Hit
GTACCAGCAGCGCCCGGGCAGTTCCCCCACCGGTGTGATTTATGAGGATA12250.13811344920581947No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA12230.13788795786017732No Hit
GGTCAGGGAGGCAGAGTTGGAGGAGGCGTCGATGGAGCCAGAGAATCGAT12170.13721148382325085No Hit
CCCCAGAGGGTCTTTTATTATCCTCATAAATCACACCGGTGGGGGAACTGCCCGGGCGCTGCTGGTACCACTGAA12130.13676050113196656No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT12090.13630951844068223No Hit
CCTTTGTTCAGTGGTACCAGCAGCGCCCGGGCAGTTCCCCCACCGGTGTGATTTATGAGGATAATAAAAGACCCT11970.13495657036682931No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG11970.13495657036682931No Hit
GTCTCATGATAGCACCACTAAAGTGTTCGGCGGAGGGACCAAACTGACCGTCCTGGGTCAGCCCAAGGCTGCCCC11860.13371636796579747No Hit
TCCCTGACCATCTCAGGCCTGAAGACCGATGACGAGGCTGACTACTATTG11800.13303989392887103No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA11740.13236341989194456No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC11720.1321379285463024No Hit
CAGGAAGCAGAGCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTT11660.13146145450937594No Hit
CTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATG11470.12931928672577547No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC11180.12604966221396424No Hit
CCTGAAGACCGATGACGAGGCTGACTACTATTGTCAGTCTCATGATAGCACCACTAAAGTGTTCGGCGGAGGGAC11140.12559867952267992No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA11120.12537318817703777No Hit
GAGCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGG10970.1236820030847216No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC10970.1236820030847216No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC10960.12356925741190054No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGTTTGGTCCCTCCGCCGAACA10940.12334376606625838No Hit
TCCTCCAACTCTGCCTCCCTGACCATCTCAGGCCTGAAGACCGATGACGA10800.1217653266467633No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC10670.1202996329000893No Hit
GGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10660.12018688722726821No Hit
GGACCAAACTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG10600.11951041319034174No Hit
CTTCAGGCCTGAGATGGTCAGGGAGGCAGAGTTGGAGGAGGCGTCGATGG10580.11928492184469959No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC10510.11849570213495206No Hit
ACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGC10500.11838295646213097No Hit
GGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGG10420.11748099107956236No Hit
GGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCGGGGTCTCCGGGGGAGACGATAACCATTTC10370.11691726271545698No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT10310.11624078867853052No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC10220.11522607762314081No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAAC10210.11511333195031975No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA10160.11454960358621435No Hit
ACTATTGTCAGTCTCATGATAGCACCACTAAAGTGTTCGGCGGAGGGACC10140.11432411224057219No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT10140.11432411224057219No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG10100.11387312954928788No Hit
GCTGGTACCACTGAACAAAGGCGCTGGCAATGCCGCCACTGGTGCGGGTG9910.11173096176568743No Hit
GACAATAGTAGTCAGCCTCGTCATCGGTCTTCAGGCCTGAGATGGTCAGG9880.1113927247472242No Hit
GACTCAGCCCCACTCTGTGTCGGGGTCTCCGGGGGAGACGATAACCATTT9850.11105448772876098No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT9850.11105448772876098No Hit
CCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCC9810.11060350503747667No Hit
GGAAGCAGAGCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTTCT9790.1103780136918345No Hit
GTTGGAGGAGGCGTCGATGGAGCCAGAGAATCGATCAGGGACCCCAGAGG9750.10992703100055021No Hit
GACCATCTCAGGCCTGAAGACCGATGACGAGGCTGACTACTATTGTCAGTCTCATGATAGCACCACTAAAGTGTT9720.10958879398208696No Hit
GTATCAACGCAGAGTACGGGGTAGCTCAGGAAGCAGAGCCTGGAGCATCT9720.10958879398208696No Hit
GTCTTTTATTATCCTCATAAATCACACCGGTGGGGGAACTGCCCGGGCGCTGCTGGTACCACTGAACAAAGGCG9600.10823584590823404No Hit
GGCCTGAAGACCGATGACGAGGCTGACTACTATTGTCAGTCTCATGATAG9470.10677015216156005No Hit
GTATCAACGCAGAGTACGGGAGCTCAGGAAGCAGAGCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTT9340.10530445841488603No Hit
CCTCTGGGGTCCCTGATCGATTCTCTGGCTCCATCGACGCCTCCTCCAACTCTGCCTCCCTGACCATCTCAGGC9230.10406425601385419No Hit
CTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCC9210.10383876466821204No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGG9210.10383876466821204No Hit
CACCAGTGGCGGCATTGCCAGCGCCTTTGTTCAGTGGTACCAGCAGCGCC9080.10237307092153802No Hit
GAGATGGTCAGGGAGGCAGAGTTGGAGGAGGCGTCGATGGAGCCAGAGAA8930.10068188582922188No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG8910.10045639448357972No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGT8890.10023090313793757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1350.053.33405339
TAAGGCG1600.053.1000229
TCGTATG1400.051.52323540
GCGAATC1400.051.12621333
GGCGAAT1650.049.5264232
CGTATGC1550.046.61697441
CACGAGA1850.045.83112721
TCTCGTA1500.045.65245438
ACTAAGG1950.043.529927
GACTAAG1950.043.50779726
TATACAC1950.043.4783673
ATGCCGT1850.043.04807344
GTCTAAG405.5320157E-542.39381
GTGGTAT2800.042.3937951
AGGGGGG3150.042.2356770
CTTATAC2050.041.3598021
CGAGACT2050.041.35980223
CGAATCT1750.040.93808434
AGACTAA2100.040.39554225
TCTCCGA2150.039.43164411
Produced by FastQC (version 0.11.5)