FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences886952
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC56820.6406209129693603No Hit
GCCCCACTCTGTGTCGGGGTCTCCGGGGGAGACGATAACCATTTCCTGCACCCGCACCAGTGGCGGCATTGCCAG53960.6083756505425322No Hit
GCCTTGGGCTGACCCAGGACGGTCAGTTTGGTCCCTCCGCCGAACACTTT52800.5952971524952873No Hit
GTGTCGGGGTCTCCGGGGGAGACGATAACCATTTCCTGCACCCGCACCAGTGGCGGCATTGCCAGCGCCTTTGTT39240.44241402014990666No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33360.3761195645311133No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC31680.35717829149717234No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31650.3568400544787091No Hit
TCTCAGGCCTGAAGACCGATGACGAGGCTGACTACTATTGTCAGTCTCAT31630.35661456313306694No Hit
GAGTGGGGCTGAGTCAGCATAAAATTGGCCCAAGAACCTGTGCAGTGAGCGAGGAGGGTGAGAAGTAGTGGAGCC26210.2955064084640432No Hit
CCACTAAAGTGTTCGGCGGAGGGACCAAACTGACCGTCCTGGGTCAGCCC26050.29370247769890595No Hit
CTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCGGGGTCTCCGGGGGAGACGATAAC25920.2922367839522319No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC25910.29212403827941086No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGTTTGGTCCCTC25470.28716322867528343No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG25260.2847955695460408No Hit
GGCCTGAGATGGTCAGGGAGGCAGAGTTGGAGGAGGCGTCGATGGAGCCA24560.27690337244856544No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24090.27160432582597477No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG23330.2630356546915729No Hit
CTTTAGTGGTGCTATCATGAGACTGACAATAGTAGTCAGCCTCGTCATCG22820.257285625377698No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21010.23687865859708304No Hit
CCCCGACACAGAGTGGGGCTGAGTCAGCATAAAATTGGCCCAAGAACCTG20900.2356384561960512No Hit
GTGGGGGAACTGCCCGGGCGCTGCTGGTACCACTGAACAAAGGCGCTGGC20880.23541296485040902No Hit
GACCCAGGACGGTCAGTTTGGTCCCTCCGCCGAACACTTTAGTGGTGCTATCATGAGACTGACAATAGTAGTCAG20860.23518747350476688No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20420.2302266639006395No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT20160.22729527640729147No Hit
GTGGTACCAGCAGCGCCCGGGCAGTTCCCCCACCGGTGTGATTTATGAGGATAATAAAAGACCCTCTGGGGTCC19910.2244766345867646No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19890.22425114324112239No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19850.2238001605498381No Hit
GAGTTGGAGGAGGCGTCGATGGAGCCAGAGAATCGATCAGGGACCCCAGA19360.2182756225816053No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19240.21692267450775238No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18820.21218735624926716No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG18800.21196186490362498No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18450.2080157663548873No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17990.20282946540511776No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG17820.20091278896715944No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17360.19572648801738987No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17270.1947117769620002No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17200.19392255725225266No Hit
CTACTATTGTCAGTCTCATGATAGCACCACTAAAGTGTTCGGCGGAGGGACCAAACTGACCGTCCTGGGTCAGCC16960.1912166611045468No Hit
CTGCTGGTACCACTGAACAAAGGCGCTGGCAATGCCGCCACTGGTGCGGGTGCAGGAAATGGTTATCGTCTCCCC16760.18896174764812526No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16310.1838881923711768No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16190.18253524429732387No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA16000.1803930765137234No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15910.17937836545833372No Hit
GCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCT15520.1749812842183117No Hit
ATAAAAGACCCTCTGGGGTCCCTGATCGATTCTCTGGCTCCATCGACGCC15230.17171165970650049No Hit
CAATAGTAGTCAGCCTCGTCATCGGTCTTCAGGCCTGAGATGGTCAGGGA15220.17159891403367938No Hit
ACCCAGGACGGTCAGTTTGGTCCCTCCGCCGAACACTTTAGTGGTGCTATCATGAGACTGACAATAGTAGTCAGC15120.17047145730546862No Hit
CTCCAACTCTGCCTCCCTGACCATCTCAGGCCTGAAGACCGATGACGAGG15060.16979498326854214No Hit
GTGCTATCATGAGACTGACAATAGTAGTCAGCCTCGTCATCGGTCTTCAG15010.16923125490443677No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14950.1685547808675103No Hit
CCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGA14820.1670890871208363No Hit
TGATAGCACCACTAAAGTGTTCGGCGGAGGGACCAAACTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGT14690.16562339337416232No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGT14600.1646086823187726No Hit
ATCATGAGACTGACAATAGTAGTCAGCCTCGTCATCGGTCTTCAGGCCTG14380.1621282775167089No Hit
GTCTTCAGGCCTGAGATGGTCAGGGAGGCAGAGTTGGAGGAGGCGTCGATGGAGCCAGAGAATCGATCAGGGACC14300.1612263121341403No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14250.1606625837700349No Hit
GTTTGGTCCCTCCGCCGAACACTTTAGTGGTGCTATCATGAGACTGACAA14230.16043709242439275No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC14150.15953512704182413No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT14130.15930963569618198No Hit
TCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATG14080.1587459073320766No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13930.15705472223976044No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13670.15412333474641243No Hit
ATTTTATGCTGACTCAGCCCCACTCTGTGTCGGGGTCTCCGGGGGAGACG13640.1537850977279492No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13590.1532213693638438No Hit
ATAGTAGTCAGCCTCGTCATCGGTCTTCAGGCCTGAGATGGTCAGGGAGG13530.15254489532691734No Hit
CTCCGGGGGAGACGATAACCATTTCCTGCACCCGCACCAGTGGCGGCATT13500.15220665830845412No Hit
GTTCCCCCACCGGTGTGATTTATGAGGATAATAAAAGACCCTCTGGGGTC13480.15198116696281197No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC13080.1474713400499689No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA12930.14578015495765273No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGTTTGGTCCCTCCGCCGAACACTTTAGTGGTG12920.14566740928483166No Hit
CCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTT12890.1453291722663684No Hit
GACTACTATTGTCAGTCTCATGATAGCACCACTAAAGTGTTCGGCGGAGGGACCAAACTGACCGTCCTGGGTCAG12850.1448781895750841No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12850.1448781895750841No Hit
GGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCC12710.14329975015558902No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12660.14273602179148365No Hit
GTACCAGCAGCGCCCGGGCAGTTCCCCCACCGGTGTGATTTATGAGGATA12560.14160856506327288No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGTTTGGTCCCTCCGCCGAACA12430.14014287131659886No Hit
CCCCAGAGGGTCTTTTATTATCCTCATAAATCACACCGGTGGGGGAACTGCCCGGGCGCTGCTGGTACCACTGAA12310.13878992324274594No Hit
GCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCG12260.13822619487864057No Hit
GAACAAAGGCGCTGGCAATGCCGCCACTGGTGCGGGTGCAGGAAATGGTTATCGTCTCCCCCGGAGACCCCGACA12190.13743697516889303No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12120.13664775545914548No Hit
GTCTCATGATAGCACCACTAAAGTGTTCGGCGGAGGGACCAAACTGACCGTCCTGGGTCAGCCCAAGGCTGCCCC12020.1355202987309347No Hit
TCCCTGACCATCTCAGGCCTGAAGACCGATGACGAGGCTGACTACTATTG11990.13518206171247146No Hit
ACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACT11880.13394185931143965No Hit
GTCTTTTATTATCCTCATAAATCACACCGGTGGGGGAACTGCCCGGGCGCTGCTGGTACCACTGAACAAAGGCG11820.13326538527451318No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11820.13326538527451318No Hit
CTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACT11600.13078498047244946No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAAC11340.12785359297910148No Hit
CCTTTGTTCAGTGGTACCAGCAGCGCCCGGGCAGTTCCCCCACCGGTGTGATTTATGAGGATAATAAAAGACCCT11310.12751535596063823No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG11310.12751535596063823No Hit
ACTCTGTGTCGGGGTCTCCGGGGGAGACGATAACCATTTCCTGCACCCGC11300.12740261028781716No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG11280.127177118942175No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT11140.12559867952267992No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA10940.12334376606625838No Hit
TCCTCCAACTCTGCCTCCCTGACCATCTCAGGCCTGAAGACCGATGACGA10900.12289278337497408No Hit
CAGGAAGCAGAGCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTT10880.12266729202933192No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT10670.1202996329000893No Hit
CCTGAAGACCGATGACGAGGCTGACTACTATTGTCAGTCTCATGATAGCACCACTAAAGTGTTCGGCGGAGGGAC10660.12018688722726821No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10650.12007414155444714No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC10590.11939766751752068No Hit
GACCATCTCAGGCCTGAAGACCGATGACGAGGCTGACTACTATTGTCAGTCTCATGATAGCACCACTAAAGTGTT10450.1178192280980256No Hit
CTTCAGGCCTGAGATGGTCAGGGAGGCAGAGTTGGAGGAGGCGTCGATGG10400.1172554997339202No Hit
CTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATG10280.11590255166006727No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC10270.11578980598724621No Hit
GCTGGTACCACTGAACAAAGGCGCTGGCAATGCCGCCACTGGTGCGGGTG10230.1153388232959619No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC10160.11454960358621435No Hit
GGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10160.11454960358621435No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC10150.11443685791339328No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC10010.1128584184938982No Hit
ACTATTGTCAGTCTCATGATAGCACCACTAAAGTGTTCGGCGGAGGGACC10000.11274567282107711No Hit
GGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCGGGGTCTCCGGGGGAGACGATAACCATTTC9950.11218194445697174No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT9930.1119564531113296No Hit
CCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCC9870.11127997907440312No Hit
GAGCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGG9810.11060350503747667No Hit
CTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCT9790.1103780136918345No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA9760.11003977667337127No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG9680.10913781129080265No Hit
GACTCAGCCCCACTCTGTGTCGGGGTCTCCGGGGGAGACGATAACCATTT9590.10812310023541297No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGT9560.10778486321694973No Hit
CCTCTGGGGTCCCTGATCGATTCTCTGGCTCCATCGACGCCTCCTCCAACTCTGCCTCCCTGACCATCTCAGGC9560.10778486321694973No Hit
GACAATAGTAGTCAGCCTCGTCATCGGTCTTCAGGCCTGAGATGGTCAGG9480.10688289783438111No Hit
GTATCAACGCAGAGTACGGGAGCTCAGGAAGCAGAGCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTT9460.10665740648873895No Hit
GAGATGGTCAGGGAGGCAGAGTTGGAGGAGGCGTCGATGGAGCCAGAGAA9410.10609367812463358No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC9410.10609367812463358No Hit
GGAAGCAGAGCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTTCT9350.10541720408770712No Hit
GGCCTGAAGACCGATGACGAGGCTGACTACTATTGTCAGTCTCATGATAG9330.10519171274206496No Hit
GGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGG9280.10462798437795957No Hit
GGACCAAACTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG9280.10462798437795957No Hit
GTATCAACGCAGAGTACGGGGTAGCTCAGGAAGCAGAGCCTGGAGCATCT9180.10350052764974879No Hit
CTCTGGCTCCATCGACGCCTCCTCCAACTCTGCCTCCCTGACCATCTCAGGCCTGAAGACCGATGACGAGGCTGA9170.10338778197692773No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT9160.10327503630410664No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC9150.10316229063128557No Hit
CTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCT8920.1005691401564008No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAG200.00785222650.9068152
GTGGTAT3150.047.4615751
TGGTATC3150.047.405292
GTATCAA18900.042.2479931
GGTATCA7000.037.8613971
TATCAAC22600.035.2893832
TCAACGC23050.034.539974
ATCAACG23150.034.4101683
CGTGCGA502.0185627E-434.02627632
GATAGTC502.0715049E-433.8766567
CAACGCA23600.033.7331125
AACGCAG23900.033.5931666
ACGCAGA25200.031.994627
CGCAGAG25400.031.4759488
CAGAGTA25600.031.23004210
AGAGTAC25500.031.21966411
ACCCGTC553.623808E-430.79695911
CGGCAAA900.004922344330.27128870
TATGTAC450.005037925730.1312683
GGTCGGT450.00505323130.11258311
Produced by FastQC (version 0.11.5)