FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1146671
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACCAGAGTGCTTCTGCTTGTGTACGAGGAG49120.42837047418134755No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACCAGAGTGCTTCTGCTTGTGTACGAGGAGCAGTAGTAAT48070.4192135320418847No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC47370.41310890394890953No Hit
GTAGTAATCAGCCTCGTCCTCAGCCTGGACCCCAGAAATGGTCAGGGAGG45340.3954054824792813No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAGCATCTCCTG44240.3858124954760346No Hit
GATTACTACTGCTCCTCGTACACAAGCAGAAGCACTCTGGTCTTCGGCGGAGGGACCAAACTGACCGTCCTAGGT41230.3595625946762411No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG39200.3418591732066129No Hit
CTCCTGGACAGTCGATCAGCATCTCCTGCACTGGAACCAGCACTGACATT38000.3313940964757982No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT37560.3275569016744995No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32530.2836907883778346No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC31590.2754931449386965No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30890.26938851684572124No Hit
CTCCTGCACTGGAACCAGCACTGACATTGGTGGTTATGACTATGTCTCCTGGTATCAGCAACACCCAGGCAAAGT29740.2593594849786905No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT29720.2591850670331769No Hit
GAGCAGTAGTAATCAGCCTCGTCCTCAGCCTGGACCCCAGAAATGGTCAGGGAGGCCGTTTTGCCAGACTTGGAG29660.25866181319663617No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG28940.2523827671581474No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26480.23092935985997726No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAG25730.2243886869032181No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC25630.2235165971756502No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG24770.21601662551856637No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24180.21087129612591582No Hit
CTACTGCTCCTCGTACACAAGCAGAAGCACTCTGGTCTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCC23860.20808060899769856No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23710.20677247440634672No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT22250.1940399643838555No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21890.19090044136461112No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21530.1877609183453667No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG21380.1864527837540149No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21380.1864527837540149No Hit
GACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAGCATCT21090.183923723544068No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21070.18374930559855443No Hit
TTTCTGGGGTCCAGGCTGAGGACGAGGCTGATTACTACTGCTCCTCGTAC21060.18366209662579763No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20510.17886560312417424No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20310.17712142366903846No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19950.17398190064979405No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19110.1666563469382238No Hit
CCCCAGAAATGGTCAGGGAGGCCGTTTTGCCAGACTTGGAGCCAGAGAAGCGATGAGAAACCCCTGAGGGCCGAT18640.1625575252186547No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAAC18610.16229589830038432No Hit
GTTTGGTCCCTCCGCCGAAGACCAGAGTGCTTCTGCTTGTGTACGAGGAGCAGTAGTAATCAGCCTCGTCCTCAG18500.16133659960005964No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC18100.1578482406897881No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17260.1505226869782178No Hit
ACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACCAGAGTGCTTCTGC17250.150435478005461No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17050.14869129855032526No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC17030.14851688060481166No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC16770.14624944731313516No Hit
GCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAGCATCTCCTGCACTGGAACCAGCA16750.14607502936762157No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16510.14398201402145863No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16330.14241225251183645No Hit
GGTTATGACTATGTCTCCTGGTATCAGCAACACCCAGGCAAAGTCCCCAAGCTCTTGATTTATGGGGTCAGTGAT16270.14188899867529572No Hit
GTCGATCAGCATCTCCTGCACTGGAACCAGCACTGACATTGGTGGTTATGACTATGTCTCCTGGTATCAGCAACA16130.14066807305670065No Hit
TCCTCAGCCTGGACCCCAGAAATGGTCAGGGAGGCCGTTTTGCCAGACTTGGAGCCAGAGAAGCGATGAGAAACC15970.13927272949259203No Hit
GGCTGAGGACGAGGCTGATTACTACTGCTCCTCGTACACAAGCAGAAGCA15850.13822622181951055No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGT15800.13779017695572662No Hit
GTCCCCAAGCTCTTGATTTATGGGGTCAGTGATCGGCCCTCAGGGGTTTCTCATCGCTTCTCTGGCTCCAAGT15540.1355227436640501No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15190.1324704296175625No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC15170.1322960116720489No Hit
GCCTGGACCCCAGAAATGGTCAGGGAGGCCGTTTTGCCAGACTTGGAGCC15040.13116229502621066No Hit
GCCCTCAGGGGTTTCTCATCGCTTCTCTGGCTCCAAGTCTGGCAAAACGG15010.1309006681079403No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14770.12880765276177736No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC14650.1277611450886959No Hit
AGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG14380.12540650282426258No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC14120.12313906953258606No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAACCT14050.12252860672328854No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13980.12191814391399101No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACCAGAGTGCTT13780.12017396445885525No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13750.11991233754058489No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13070.11398212739312322No Hit
ACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAGCATCTCCTGCA12980.11319724663831213No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT12770.11136585821041955No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12740.11110423129214919No Hit
GGTATCAGCAACACCCAGGCAAAGTCCCCAAGCTCTTGATTTATGGGGTC12730.1110170223193924No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA12700.11075539540112203No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG12540.1093600518370134No Hit
CAGCAACACCCAGGCAAAGTCCCCAAGCTCTTGATTTATGGGGTCAGTGATCGGCCCTCAGGGGTTTCTCATCG12090.10543564806295791No Hit
GTGATCGGCCCTCAGGGGTTTCTCATCGCTTCTCTGGCTCCAAGTCTGGC11950.10421472244436286No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT11810.10299379682576781No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA11610.10124961737063203No Hit
CATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC11580.10098799045236166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21250.042.4296231
GATCGGA350.001287663539.79641750
TATCAAC23700.038.183652
ATCAACG24350.037.164373
TCAACGC25100.036.190974
CAACGCA25050.036.125855
ACGTTAA400.002449743234.91122453
AACGCAG26150.034.7377976
AGAGTAC27900.032.31083311
CGCAGAG28300.032.0973058
CAGAGTA28600.031.52001210
ACGCAGA29350.030.949027
GCAGAGT29300.030.8844039
AGTACGG22750.029.34204513
GAGTACG23750.028.10658812
CCTTACG500.00746569627.80105847
GGCGCTA500.007588454527.70831342
TACATCG500.00783541927.5270715
GTGGTAT3150.027.3121761
ATGCGTA550.00867698726.95717668
Produced by FastQC (version 0.11.5)