FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1146671
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACCAGAGTGCTTCTGCTTGTGTACGAGGAG46900.40901008222934043No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC45790.3993298862533369No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAGCATCTCCTG44430.38746946595841353No Hit
GTAGTAATCAGCCTCGTCCTCAGCCTGGACCCCAGAAATGGTCAGGGAGG44270.3860741223943049No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACCAGAGTGCTTCTGCTTGTGTACGAGGAGCAGTAGTAAT43200.37674276230932846No Hit
GATTACTACTGCTCCTCGTACACAAGCAGAAGCACTCTGGTCTTCGGCGGAGGGACCAAACTGACCGTCCTAGGT39230.3421208001248832No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG36200.3156964813795762No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT35820.3123825404148182No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34010.29659771634583937No Hit
CTCCTGGACAGTCGATCAGCATCTCCTGCACTGGAACCAGCACTGACATT33330.2906675061983778No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG29600.25813855936009544No Hit
CTCCTGCACTGGAACCAGCACTGACATTGGTGGTTATGACTATGTCTCCTGGTATCAGCAACACCCAGGCAAAGT29430.25665600682323003No Hit
GAGCAGTAGTAATCAGCCTCGTCCTCAGCCTGGACCCCAGAAATGGTCAGGGAGGCCGTTTTGCCAGACTTGGAG29190.25456299147706707No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29010.2529932299674449No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28850.25159788640333625No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC28620.2495920800299301No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAG27050.23590027130711422No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26740.2331967931516538No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23920.2086038628342393No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG23230.20258644371402088No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC23130.20171435398645296No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23010.2006678463133715No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22150.19316787465628765No Hit
CTACTGCTCCTCGTACACAAGCAGAAGCACTCTGGTCTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCC22050.19229578492871977No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21810.1902027695825568No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21780.18994114266428644No Hit
GACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAGCATCT21560.18802254526363707No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21320.18592952991747416No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20720.1806969915520668No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20480.17860397620590387No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20160.17581328907768662No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT20000.174417945513578No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19860.17319701989498296No Hit
TTTCTGGGGTCCAGGCTGAGGACGAGGCTGATTACTACTGCTCCTCGTAC19610.17101679557606325No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19100.166569137965467No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAAC18360.1601156739814646No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC17910.1561912702074091No Hit
GTTTGGTCCCTCCGCCGAAGACCAGAGTGCTTCTGCTTGTGTACGAGGAGCAGTAGTAATCAGCCTCGTCCTCAG17730.1546215086977869No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17370.15148198567854249No Hit
GCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAGCATCTCCTGCACTGGAACCAGCA16970.14799362676827094No Hit
GGTTATGACTATGTCTCCTGGTATCAGCAACACCCAGGCAAAGTCCCCAAGCTCTTGATTTATGGGGTCAGTGAT16710.1457261934765944No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16680.14546456655832404No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16680.14546456655832404No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC16670.14537735758556727No Hit
CCCCAGAAATGGTCAGGGAGGCCGTTTTGCCAGACTTGGAGCCAGAGAAGCGATGAGAAACCCCTGAGGGCCGAT16560.1444180588852426No Hit
GGCTGAGGACGAGGCTGATTACTACTGCTCCTCGTACACAAGCAGAAGCA16170.1410169089477278No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16160.14092969997497104No Hit
GTCGATCAGCATCTCCTGCACTGGAACCAGCACTGACATTGGTGGTTATGACTATGTCTCCTGGTATCAGCAACA15620.13622041544610441No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC15510.13526111674577976No Hit
GCCCTCAGGGGTTTCTCATCGCTTCTCTGGCTCCAAGTCTGGCAAAACGG15460.1348250718819958No Hit
ACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACCAGAGTGCTTCTGC15420.13447623599096864No Hit
ACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAGCATCTCCTGCA15210.13264484756307607No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGT15050.13124950399896745No Hit
GTCCCCAAGCTCTTGATTTATGGGGTCAGTGATCGGCCCTCAGGGGTTTCTCATCGCTTCTCTGGCTCCAAGT14730.1284588168707502No Hit
AGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG14720.1283716078979934No Hit
GCCTGGACCCCAGAAATGGTCAGGGAGGCCGTTTTGCCAGACTTGGAGCC14660.12784835406145267No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14580.12715068227939838No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14580.12715068227939838No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC14420.12575533871528974No Hit
TCCTCAGCCTGGACCCCAGAAATGGTCAGGGAGGCCGTTTTGCCAGACTTGGAGCCAGAGAAGCGATGAGAAACC14280.12453441309669469No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14100.12296465158707248No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13910.12130768110469349No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAACCT13840.12069721829539598No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13760.11999954651334167No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACCAGAGTGCTT13590.11851699397647626No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13570.11834257603096268No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13070.11398212739312322No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12960.11302282869279856No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT12650.11031935053733809No Hit
GTGATCGGCCCTCAGGGGTTTCTCATCGCTTCTCTGGCTCCAAGTCTGGC12520.10918563389149982No Hit
GGTATCAGCAACACCCAGGCAAAGTCCCCAAGCTCTTGATTTATGGGGTC12260.10691820059982331No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12150.10595890189949865No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCT12030.10491239422641717No Hit
CAGCAACACCCAGGCAAAGTCCCCAAGCTCTTGATTTATGGGGTCAGTGATCGGCCCTCAGGGGTTTCTCATCG11900.10377867758057892No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA11710.10212170709819991No Hit
CTCCTGGTATCAGCAACACCCAGGCAAAGTCCCCAAGCTCTTGATTTATG11560.10081357250684808No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA11560.10081357250684808No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAGAC11530.10055194558857772No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG150.002392313468.7315
GTATCAA23000.044.942151
ATCAACG26400.039.0806733
TATCAAC26450.039.0306432
TCAACGC27050.037.989584
CAACGCA27050.037.9862635
AACGCAG27400.037.3756186
AGAGTAC29450.034.7739211
CGCAGAG29650.034.5393568
ACGCAGA30400.033.6872337
CAGAGTA30750.033.30380210
GCAGAGT31550.032.3504079
CGTATAT901.6525155E-731.33271662
AGTACGG26900.029.38314213
GAGTACG27350.028.77403812
TACGGGG19750.027.66640315
GCCAGAG26650.027.3683870
ACGGGGG11200.027.00264416
TACGGGC3100.026.60554715
CGATTTA3257.2759576E-1225.81831670
Produced by FastQC (version 0.11.5)