FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1489648
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGGCTGGCCTCCGAACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT52940.3553859703768944No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46100.30946908262891637No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38910.2612026465312611No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31420.21092231184816815No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30910.20749868425292417No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29970.2011884686852196No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCA29120.1954824226931463No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29080.1952139028817546No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATG28310.1900448965124647No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCA28310.1900448965124647No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27340.18353329108621633No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27240.18286199155773714No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATGTTGCAGTTTATTTCTGTCAGCAGTATAATGGCTGG25820.17332953825333233No Hit
CCATTATACTGCTGACAGAAATAAACTGCAACATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC25370.1703086903751759No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25340.17010730051663212No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT24530.1646697743359505No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23610.15849381867394177No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22490.1509752639549746No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGTT21910.1470817266897952No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21680.145537737774293No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21320.14312105947176784No Hit
GGCCAAAAGTGTTCGGAGGCCAGCCATTATACTGCTGACAGAAATAAACTGCAACATCTTCAGACTGCAGGCTG20900.14030160145215514No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20880.1401673415464593No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20700.1389590023951967No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20190.13553537479995276No Hit
GTGTTAGCAGCGACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCCCCC19170.12868811960946477No Hit
GTCTGTGTCTCCAGGGGAAAGAGCTACCGTCTCCTGTAGGGCCAGTCAGAGTGTTAGCAGCGACTTAGCCTGGTA18860.12660709107117923No Hit
CATCTATGGTGCATTCAACAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA18520.1243246726743499No Hit
GTCTGAAGATGTTGCAGTTTATTTCTGTCAGCAGTATAATGGCTGGCCTC18030.12103530498480178No Hit
GTCTCCGGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCTACCGTCTCCTGTAGGGCCAGTCAGAGTGTTAGCAG18000.120833915126258No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTTCGGAGGCCAGCCATTATACTG17420.1169403778610786No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCC17390.11673898800253482No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17020.11425517974716175No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16910.11351675026583462No Hit
GCAGAAATAGTGCTGACGCAGTCTCCGGCCACCCTGTCTGTGTCTCCAGG16760.11250980097311579No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16460.11049590238767816No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCACTGCAGAAATAGTG16410.11016025262343856No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16330.1096232130006552No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15090.10129909884751297No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14960.10042640946049No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14950.10035927950764206No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14950.10035927950764206No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)