FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1489648
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGGCTGGCCTCCGAACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT52820.35458041094271936No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46040.30906630291182885No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33350.22387839274781693No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31550.2117950012351911No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30450.2044107064219198No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28520.19145462552227105No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATG28170.18910507717259378No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCA27980.18782960806848328No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27800.1866212689172207No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCA26930.18078096301945157No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATGTTGCAGTTTATTTCTGTCAGCAGTATAATGGCTGG26270.17635038613148876No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25850.1735309281118761No Hit
CCATTATACTGCTGACAGAAATAAACTGCAACATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC24510.16453551443025466No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23740.15936650806096475No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22350.1500354446151037No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT22210.14909562527523282No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21930.147215986595491No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21780.1462090373027722No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21420.14379235900024703No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21160.1420469802262011No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20930.1405029913106989No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20880.1401673415464593No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGTT20630.13848909272526128No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20090.13486407527147354No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19840.1331858264502755No Hit
GGCCAAAAGTGTTCGGAGGCCAGCCATTATACTGCTGACAGAAATAAACTGCAACATCTTCAGACTGCAGGCTG19520.13103766795914204No Hit
GTCTGTGTCTCCAGGGGAAAGAGCTACCGTCTCCTGTAGGGCCAGTCAGAGTGTTAGCAGCGACTTAGCCTGGTA18490.12412328281580616No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18060.12123669484334554No Hit
CATCTATGGTGCATTCAACAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA17900.12016261559777881No Hit
GTCTGAAGATGTTGCAGTTTATTTCTGTCAGCAGTATAATGGCTGGCCTC17800.1194913160692996No Hit
GTGTTAGCAGCGACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCCCCC17570.11794732715379741No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17440.11707463776677443No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17420.1169403778610786No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTTCGGAGGCCAGCCATTATACTG17030.11432230970000966No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCC16710.1121741512088762No Hit
GCAGAAATAGTGCTGACGCAGTCTCCGGCCACCCTGTCTGTGTCTCCAGG15660.10512550615984448No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15490.10398429696142981No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCACTGCAGAAATAGTG15320.10284308776301516No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15300.10270882785731932No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15270.10250743799877554No Hit
GTCTCCGGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCTACCGTCTCCTGTAGGGCCAGTCAGAGTGTTAGCAG15050.10103057903612128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13800.045.3487661
GTATCAA35400.041.573221
TATCAAC39950.036.8784982
TCAACGC41800.034.954024
ATCAACG41850.034.9286733
CAACGCA42650.034.256255
AACGCAG43650.033.6285976
CGCAGAG47400.030.8957528
ACGCAGA48950.030.0575667
AGAGTAC48750.030.04017611
CTACGGA5250.029.64462369
GTGGTAT4800.029.37081
GCAGAGT51700.028.3260869
CAGAGTA53150.027.68237110
ATATCGC500.00796224527.4371646
TTAACGG1306.355549E-926.5207635
AGTACGG39200.026.24728813
AACGGCC1457.021299E-1026.17792137
GAGTACG39550.026.0150112
CGCGGTA1358.8348315E-925.71290243
Produced by FastQC (version 0.11.5)