FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429426
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT5230.12179048311001196No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCT306.3549425E-445.9293828
GTGGTAT2950.043.1846241
TGGTATC2700.042.0774152
GTATCAA17700.040.2667471
GGTATCA7200.037.778441
TATCAAC20100.036.139762
TCGTTAA851.7683476E-434.78920770
AACGCAG21000.034.0951276
ACGCAGA22200.032.248397
CGCAGAG22550.031.7478648
AGAGTAC22750.031.62005211
CAGAGTA22750.031.62005210
GCAGAGT23550.030.545919
ATCAACG23700.030.3596533
TCAACGC23700.030.3596534
CAACGCA23900.029.9615525
CGGGTAT703.9045597E-529.505417
GAGTACG20100.027.9118912
AGTACGG20150.027.84262813
TACGGGC4150.027.36929115
Produced by FastQC (version 0.11.5)