FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523979
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44830.29416415843000465No Hit
GTTTTATACACCTCCCACAGTAAGAACTACTTAGCTTGGTACCAGCAGAG35220.23110554672997463No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33820.2219190684386071No Hit
CTCCTAAACTCCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCT27380.17966126829831647No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26800.17585544157760702No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25650.16830940583826942No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25090.1646348145217224No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24910.16345369588426087No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24050.15781057350527794No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATTAAACGAACTGTGGCTGCACCA23270.15269239274294463No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23250.15256115733878223No Hit
GTATTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGC22060.14475265079111982No Hit
GTTTAGGAGGCTGCCCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCTTACTGTGGGAGGTGTATAAAACACT21890.1436371498557395No Hit
ATAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATTAAACGA21240.1393719992204617No Hit
GTATTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT20940.13740346815802582No Hit
GTACCAGCAGAGACCAGGGCAGCCTCCTAAACTCCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCTGA20730.13602549641432066No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20620.1353037016914275No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19670.12907001999371384No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18950.12434554544386767No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18680.12257386748767535No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGCTTGGTCCCCTGGCCAAAAGTG18110.11883365846904716No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC17970.11791501063991039No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17860.11719321591701723No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17340.11378109540879501No Hit
GACCAGGGCAGCCTCCTAAACTCCTCATTTACTGGGCTTCTACCCGGGAA17290.11345300689838901No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCGCCATCAACTGCAGGTCCAGCCAGAGTGTTTTATA17280.11338738919630781No Hit
TTATAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTT17020.1116813289421967No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16970.11135324043179072No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16950.11122200502762833No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16370.10741617830691892No Hit
GTGTTGCAGACCCAGGTCTTCACTTCTCTGTTGCTCTGGATCTCTGGTGC16180.10616944196737618No Hit
ATTATAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATTAAA16000.10498832332991465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC42100.034.979312
TCACGCG501.8584474E-434.5102735
ATCAACG42800.034.1660463
TCAACGC43450.033.5757454
CAACGCA44000.033.1560485
AACGCAG44550.032.746716
ACGCAGA49150.029.7480147
AGAGTAC50250.029.16623511
CGCAGAG50850.028.7534898
CAGAGTA51800.028.22708110
GCAGAGT53800.027.2416959
GTATCAA61500.025.7922741
AGTACGG40200.025.41775713
GAGTACG40850.025.26597212
TCGTATG1151.6421691E-624.09278140
CTCGTAT1352.5787813E-723.07443439
TGTTAGA10200.022.90447870
TAAGGCG1554.1691237E-822.21716129
GTACGGG45950.022.16220714
GAGCAAA19850.021.42662670
Produced by FastQC (version 0.11.5)