FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523979
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43260.28386217920325674No Hit
GTTTTATACACCTCCCACAGTAAGAACTACTTAGCTTGGTACCAGCAGAG29110.19101313075836346No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26610.17460870523806432No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26110.17132782013400447No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25830.16949052447573096No Hit
CTCCTAAACTCCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCT25690.1685718766465942No Hit
GTATTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT25370.1664721101799959No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25160.1650941384362908No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24000.15748248499487197No Hit
ATAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATTAAACGA22300.14632747564106854No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATTAAACGAACTGTGGCTGCACCA21300.1397657054329489No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21260.1395032346246241No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21250.1394376169225429No Hit
GTTTAGGAGGCTGCCCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCTTACTGTGGGAGGTGTATAAAACACT21000.13779717437051298No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19820.13005428552493178No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19650.12893878458955144No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19080.1251985755709232No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA18330.12027724791483346No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGCTTGGTCCCCTGGCCAAAAGTG17770.11660265659828646No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17760.11653703889620527No Hit
GTATTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGC17750.11647142119412407No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17320.1136498600046326No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17140.11246874136717107No Hit
GTACCAGCAGAGACCAGGGCAGCCTCCTAAACTCCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCTGA17120.11233750596300868No Hit
TTATAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTT17120.11233750596300868No Hit
ATTATAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATTAAA16680.10945032707143601No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16330.10715370749859415No Hit
GAGTATTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT15850.1040040577986967No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15750.10334788077788473No Hit
GACCAGGGCAGCCTCCTAAACTCCTCATTTACTGGGCTTCTACCCGGGAA15480.10157620282169241No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15470.10151058511961124No Hit
GGCCAAAAGTGTACGGAGTATTATAATATTGCTGACAGTAATAAACTGCC15370.10085440809879927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC42550.035.856122
ATCAACG42950.035.3378033
TCAACGC43350.034.9199144
CAACGCA44900.033.7133335
AACGCAG45550.033.3829546
AGAGTAC50200.030.56421711
ACGCAGA49800.030.534017
GTATCAA53200.030.3247911
CGCAGAG50750.030.0300718
CAGAGTA51700.029.61104810
GCAGAGT52400.029.1499759
AGTACGG40700.026.05994613
GAGTACG40950.025.9846712
AGTACTT19600.023.99241413
GAGTACT19950.023.57149312
TACGGGC7150.023.52568415
GTACGGG47750.022.2123514
GGTATCA30200.020.616181
GCCTAAT2758.4070416E-720.40581370
CAGTTCG16050.020.20097770
Produced by FastQC (version 0.11.5)