FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502848
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA27300.5429075983199695No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT23330.4639572992236223No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC21970.4369113529336897No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG21410.42577478681430575No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATATT21270.42299064528445973No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG20430.4062857961053838No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC19300.3838137966144839No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC18460.3671089474354079No Hit
GATTATTACTGCTGCTCATGTGCAGGTAGTGGCACGAATGTCTTCGGAAATGGGACCAGGGTCACCGTCCTCCGT17630.3506029655084638No Hit
GTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG17600.35000636375206823No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGGAGGACGGTGACCCTGGTCCCATTTCCGAAGACATTCGTGCCA16380.3257445589919817No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATATTGGGGGTTATAACCTTGTCTCCTGGTACCAACAGCA16120.32057401043655337No Hit
CTCCTGCACTGGAACCAGCAGTGATATTGGGGGTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGCCAAAGC15900.31619893088965256No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC14910.2965110729285987No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14900.2963122056764668No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG14480.2879597810869289No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT14060.2796073564973908No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT13320.26489117983963345No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG13030.2591240295278096No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC12620.25097047219040347No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12130.24122597683594246No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC12050.23963503881888762No Hit
GCCTTGGGCTGACGGAGGACGGTGACCCTGGTCCCATTTCCGAAGACATTCGTGCCACTACCTGCACATGAGCAG12000.23864070255822833No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11640.23148148148148145No Hit
GGGTCACCGTCCTCCGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAG11570.2300894107165585No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGC11550.22969167621229475No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC11370.22611206567392134No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC10810.21497549955453735No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG10640.2115947562682958No Hit
GGTACCAACAGCACCCAGCCAAAGCCCCCAAAGTCATGATTTATGAGGTC10530.20940721649484537No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC10480.20841288023418608No Hit
CGGTGACCCTGGTCCCATTTCCGAAGACATTCGTGCCACTACCTGCACATGAGCAGCAGTAATAATCAGCCTCGT10460.20801514572992238No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC10430.20741854397352677No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA10140.20165139366170293No Hit
ACCTGCACATGAGCAGCAGTAATAATCAGCCTCGTCGTCAGCCTGGAGCC10070.20025932289677995No Hit
GTACCAACAGCACCCAGCCAAAGCCCCCAAAGTCATGATTTATGAGGTCA9760.19409443808069238No Hit
GTATCAACGCAGAGTACGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG9670.19230463281150567No Hit
GTAATAGACGGCCGTGTCTTCAGATCTCAGGCTCCTCAACTCCATGTAGG9380.18653748249968183No Hit
GTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGCCAAAGCCCCCAAA9100.18096919943998982No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG9050.17997486317933054No Hit
GACGGAGGACGGTGACCCTGGTCCCATTTCCGAAGACATTCGTGCCACTACCTGCACATGAGCAGCAGTAATAAT8850.1759975181366934No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC8810.17520204912816598No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC8790.17480431462390225No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8750.17400884561537483No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG8670.17241790759831996No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG8560.17023036782486953No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT8560.17023036782486953No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT8530.16963376606847397No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA8430.1676450935471554No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG8340.1658552882779687No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG8050.16008813796614482No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGC8040.159889270714013No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT7990.1588949344533537No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA7890.15690626193203513No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT7810.15531532391498026No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7740.15392325315005728No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7730.15372438589792542No Hit
GTCAATAAGCGGCCGTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAAT7720.15352551864579356No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA7590.1509402443680794No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC7590.1509402443680794No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTC7530.14974704085528828No Hit
GTCTGGGGCTGAGGTGAAGAAGCCGGGGTCCTCGGTGAAAGTCTCCTGCAAGGCCTCTGGAGGCACCTTCAGCAA7360.1463662975690467No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG7180.14278668703067326No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC7130.14179235077001398No Hit
CAACAGCACCCAGCCAAAGCCCCCAAAGTCATGATTTATGAGGTCAATAAGCGGCCGTCAGGGGTTTCTAATCG7070.14059914725722286No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC6960.13841160748377243No Hit
TGTGCAGGTAGTGGCACGAATGTCTTCGGAAATGGGACCAGGGTCACCGT6950.13821274023164057No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG6930.13781500572737687No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG6830.1358263332060583No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC6830.1358263332060583No Hit
GCAGTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCAGAGATTGTCAGGG6820.13562746595392644No Hit
GTCATGATTTATGAGGTCAATAAGCGGCCGTCAGGGGTTTCTAATCGCTT6810.13542859870179458No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACGGAGGACGG6740.13403652793687157No Hit
TCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCC6720.13363879343260787No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT6690.13304219167621228No Hit
CTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCC6650.13224672266768486No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC6610.13145125365915744No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG6610.13145125365915744No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG6540.13005918289423446No Hit
ATATTGGGGGTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGCCAAA6530.12986031564210257No Hit
GTGATATTGGGGGTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGCC6380.12687730686012472No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT6380.12687730686012472No Hit
GTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCC6290.125087501590938No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT6250.1242920325824106No Hit
GGGTTGGCCTTGGGCTGACGGAGGACGGTGACCCTGGTCCCATTTCCGAA6220.12369543082601503No Hit
GCCAAAGCCCCCAAAGTCATGATTTATGAGGTCAATAAGCGGCCGTCAGG6130.12190562555682832No Hit
GTATCAACGCAGAGTACGGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG6040.12011582028764159No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA6000.11932035127911417No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG5960.11852488227058675No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC5960.11852488227058675No Hit
CTACCTGCACATGAGCAGCAGTAATAATCAGCCTCGTCGTCAGCCTGGAG5950.11832601501845488No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA5890.11713281150566374No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC5810.11554187348860889No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG5810.11554187348860889No Hit
GTGCAGGTAGTGGCACGAATGTCTTCGGAAATGGGACCAGGGTCACCGTCCTCCGTCAGCCCAAGGCCAACCCCA5790.11514413898434518No Hit
CCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAG5780.11494527173221332No Hit
CTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCC5760.11454753722794961No Hit
CCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACA5730.11395093547155402No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT5720.11375206821942216No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATTAGAAAC5710.11355320096729032No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG5690.11315546646302661No Hit
CTTATTGACCTCATAAATCATGACTTTGGGGGCTTTGGCTGGGTGCTGTTGGTACCAGGAGACAAGGTTATAACC5640.11216113020236732No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG5630.11196226295023545No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC5630.11196226295023545No Hit
GTCTATTACTGTGCGAGAACCTATTATTATGGTTCGGGGAGTCCACCAGT5620.11176339569810362No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC5570.11076905943744432No Hit
GCACTGGAACCAGCAGTGATATTGGGGGTTATAACCTTGTCTCCTGGTAC5560.11057019218531246No Hit
GGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC5550.1103713249331806No Hit
AGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCT5500.10937698867252131No Hit
GTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAG5490.10917812142038946No Hit
GTCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGGCTGACGACG5480.1089792541682576No Hit
CCCTGGTCCCATTTCCGAAGACATTCGTGCCACTACCTGCACATGAGCAGCAGTAATAATCAGCCTCGTCGTCAG5460.10858151966399389No Hit
TCTCTGGGCTCCAGGCTGACGACGAGGCTGATTATTACTGCTGCTCATGT5460.10858151966399389No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG5420.10778605065546647No Hit
CATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCC5390.1071894488990709No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGG5370.10679171439480718No Hit
GGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGG5350.10639397989054347No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACGGAG5350.10639397989054347No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCT5320.1057973781341479No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA5300.10539964362988419No Hit
GCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCT5290.10520077637775233No Hit
CTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG5290.10520077637775233No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC5250.1044053073692249No Hit
GGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACGGAGGACGGT5250.1044053073692249No Hit
GCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG5230.10400757286496119No Hit
GTCCTCCGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC5210.10360983836069745No Hit
GGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGT5170.10281436935217003No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGA5120.10182003309151075No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG5110.10162116583937891No Hit
TCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGT5090.10122343133511517No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG5090.10122343133511517No Hit
GGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGAC5030.10003022782232404No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGACGA300.003179724663.95697470
GTGGTAT1100.059.6247521
TGGTATC1100.059.6188242
GTACACG200.006960850752.47875663
AAGTAGC200.007126008652.1685151
ACCCTAT555.966913E-446.51416470
GGTATCA3800.044.5121081
GTATCAA10600.043.6379741
TATCAAC12050.038.3831182
GGGCAGT1200.037.396231
ATCAACG13250.034.906913
CCGAAAT400.002490753534.78946352
TCAACGC13350.034.6454354
AACGCAG13900.033.274576
CGCAGAG14800.031.2511188
TACGGGT1550.031.17588615
ACGGGTG1001.439912E-831.06454316
AAATTGA450.00435096431.04532661
ACGCAGA14900.031.0413787
AGAGTAC14750.030.88903811
Produced by FastQC (version 0.11.5)