FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1255134
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38500.040.0004961
GGTATCA15300.038.9559021
TCAACGC43300.035.1567274
TATCAAC44000.035.114962
ATCAACG43500.035.0908553
CAACGCA44550.034.2474175
AACGCAG45750.033.649576
GTGGTAT5800.033.264321
ACGCAGA48050.031.9673547
CGCAGAG49000.031.417718
AGAGTAC51100.030.19381511
CAGAGTA51400.029.8838810
TGGTATC6600.029.1972942
GCAGAGT52700.029.1467069
ATAACGC605.171769E-428.98347544
AGTACGG39650.025.56650713
GAGTACG41000.024.72468412
GTACTTT22150.023.7361514
ACGGGCT3400.023.24749216
AGTACTT22900.022.95876513
Produced by FastQC (version 0.11.5)