FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779033
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10600.042.4647031
GTATCAA28600.041.850541
GTGGTAT3800.040.3821071
TATCAAC33050.036.4149442
TGGTATC4500.034.8515172
ATCAACG35450.033.9496153
AACGCAG35850.033.570826
TCAACGC35900.033.524064
CAACGCA36550.032.927875
CGCAGAG37700.031.8309678
ACGCAGA37950.031.7131447
GTCTAAC553.4862227E-431.0004041
AGAGTAC38850.030.6254811
CAGAGTA39350.030.23633810
GCAGAGT40550.029.7619179
ACGCATT850.006025915529.04395170
TACGGGC4650.026.39356415
AGTACGG33300.026.20862613
GAGTACG33600.025.97461912
TACGGGG10750.024.1020315
Produced by FastQC (version 0.11.5)