FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences626431
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9940.15867669384177988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13700.042.0058141
GGTATCA5550.037.2539021
TCAACGC15600.037.0454524
CAACGCA16000.036.1193125
ATCAACG16200.035.6790923
AACGCAG16850.034.297276
ACGCAGA17000.033.9946487
TATCAAC17050.033.9274522
AGAGTAC17700.033.03891811
CGCAGAG17750.032.5582548
CAGAGTA18500.031.61020910
AGTACGG17500.028.69888313
GCAGAGT20550.028.4568799
GAGTACG18250.027.89645812
TCTATAC658.7933574E-426.465263
GTACGGG19500.025.75540714
ACGGGTA1401.4279067E-824.57292416
TACGGGG8550.024.54222515
TACGGGA5550.024.17251215
TACGGGC4200.022.93289815
Produced by FastQC (version 0.11.5)