FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1144303
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTG33880.2960754275746896No Hit
CCATAGTAGAGCAGGCAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAATGTCAGGGCAGCTTTGCCC31010.27099465788344523No Hit
CTCCTGGACTCCTGCCCGTTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACATTGTCAGGTGTGCAGC30510.2666251858117999No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTGAGCACCACCATAGTAGAGCAGGCAGTAATACTCAG28900.25255548574110176No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA27580.24102007947195805No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC26260.22948467320281432No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT25490.22275568621248043No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC24720.21602669922214657No Hit
GTCCAGGAGTGTTTGTTGCTTGTACTATAAATCAGTGACCTGGGTGCTTG23890.2087733755832153No Hit
GTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAATGTCAGGGCAG22330.19514062271968177No Hit
CTACTATGGTGGTGCTCAGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC22280.19470367551251724No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20890.18255654315334313No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20420.1784492394059965No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19960.17442932510008277No Hit
GAGTATTACTGCCTGCTCTACTATGGTGGTGCTCAGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG19950.17434193565864986No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19380.16936073749697414No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT18990.16595254928109077No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18120.15834966787642782No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG17800.1555532057505748No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17790.1554658163091419No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC17280.15100895479606363No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17090.1493485554088384No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16760.14646470384155247No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16720.14611514607582082No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC16550.1446295255714614No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16190.14148350567987675No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15820.1382500963468592No Hit
CAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAATGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGC15570.1360653603110365No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15470.13519146589670744No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15390.13449235036524415No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA15210.13291934041945183No Hit
CTACATACACTGGGTGCGACAGGCCCCTGGACAAGGACTTGAGTGGATGGGATGGAGCAACCCTAACAGCGGTGG15050.13152110935652533No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAACACTGGAGCAGTCACCAGTGGTTA15020.1312589410322266No Hit
GTATTACTGCCTGCTCTACTATGGTGGTGCTCAGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC14950.13064721494219625No Hit
CCACCATAGTAGAGCAGGCAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAATGTCAGGGCAGCTTTG14580.12741380560917867No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14250.12452995404189275No Hit
GCCTGAGGACGAGGCTGAGTATTACTGCCTGCTCTACTATGGTGGTGCTCAGGTGTTCGGCGGAGGGACCAAG14080.12304433353753333No Hit
GTCTGGGGCAGAGGTGAAGAAGCCTGGGGCCTCAGTGAGGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAA13950.12190827079890554No Hit
GATTTATAGTACAAGCAACAAACACTCCTGGACTCCTGCCCGTTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGC13730.11998570308738157No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC13730.11998570308738157No Hit
TCCTCAGGCTGCACACCTGACAATGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAAACGGGCAGGAGTC13680.11954875588021704No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13660.11937397699735124No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13510.11806313537585762No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13420.11727663040296146No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG13290.11614056766433366No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13210.11544145213287041No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13060.11413061051137678No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12730.11124675894409086No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12510.1093241912325669No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12240.1069646763138784No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG12150.10617817134098224No Hit
CAATAATACACGGCCGTGTCGTCAGATGTCAGCCTGCTCAGGTCCATGAA12040.10521688748522026No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11640.10172130982790398No Hit
GTCCAATTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC11510.10058524708927619No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCTCAGAC11480.10032307876497745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8350.048.399351
GTATCAA21850.042.840841
TATCAAC24750.037.6749572
GTGGTAT2300.037.5450171
ATCAACG26000.035.8636633
TCAACGC26250.035.6536644
CAACGCA26700.035.052765
AACGCAG27450.034.346666
TGGTATC2650.032.5805442
CAGAGTA29400.031.82806610
AGAGTAC29450.031.77402911
CGCAGAG29700.031.6228318
AATCGAG800.00404114531.51876470
CCGTTAA553.239993E-431.39168416
TATTACG450.004603498630.6927477
GCAGAGT31500.030.1446659
ACGCAGA31550.029.9874487
CGAACTA605.378745E-428.78954729
ATATATA756.1206156E-527.6283022
TCTATAC658.605416E-426.5656763
Produced by FastQC (version 0.11.5)