FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1291908
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCGTCAGATCTCAGCCTCCTCAGCTCCATGTAGGCTGTGCTGATGGACGTGTCCCTGGT46100.3568365549249637No Hit
CTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACCCTAACAGTGGTGG42040.3254101685259322No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT33250.2573712679231029No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32560.25203033033311967No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAGGCACTCAGGCTGCTGTCCCATGTT30290.2344594197110011No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAGGCACTCAGG29980.23205986804013906No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC29720.23004734083231934No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT29350.22718335980580662No Hit
GTGTATATAGTAGCCGGTGAAGGTGTATCCAGAAGCCTTACAGGAGACCTTCACTGAGGCCCCAGGCTTCTTCAC28610.22145539775278114No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT28570.22114577818234735No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGTAAGGCTTCTGGATACACCTTCACCGG28380.21967508522278675No Hit
GTGTATTACTGTGCGAGAGTTCGTATGGTTCGGGGAGTTACATTCCATTA27750.21479857698845425No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC26130.20225898438588508No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG25400.19660842722546806No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG24630.19064825049461726No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG24520.1897967966759243No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22870.1770249893955297No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC22140.17137443223511273No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA22000.1702907637385944No Hit
GCCTACATGGAGCTGAGGAGGCTGAGATCTGACGACACGGCCGTGTATTA21980.1701359539533775No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG21280.16471761147078584No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT21260.16456280168556894No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20530.15891224452515193No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT20100.15558383414298851No Hit
ACTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG20060.1552742145725547No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA19370.14993327698257153No Hit
CCCTAACAGTGGTGGCACAAACTATGCACAGAAGTTTCAGGGCTGGGTCACCATGACCAGGGACACGTCCATCAG18920.14645005681519116No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18530.14343126600346154No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC18420.1425798121847686No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC18070.13987064094347276No Hit
CTGTTAGGGTTGATCCATCCCATCCACTCAAGCCCTTGTCCAGGGGCCTG18010.13940621158782204No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT17920.138709567554346No Hit
GTCCTCTAGAGAATCCCCTGAGAGCTCCGTTCCTCACCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGGCAG17910.13863216266173753No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17790.1377033039504361No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA17700.13700665991696004No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC17700.13700665991696004No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG17230.13336862996436277No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG16900.13081426850828387No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16880.13065945872306695No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG16840.13034983915263318No Hit
CAGTAATACACGGCCGTGTCGTCAGATCTCAGCCTCCTCAGCTCCATGTA16720.12942098044133174No Hit
GTCCCATGTTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGG16610.12856952662263876No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT16340.12647959452221055No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACCCTAACAGTGGT15930.12330599392526402No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15760.12199011075092034No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGA15530.12020979822092595No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15400.11920353461701608No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA15380.11904872483179917No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC15320.11858429547614846No Hit
CCCAGGAACAGCCCCCAAACTCCTCATCTATGAAAATAATGAGCGACCCTCAGGGATTCCTGACCGATTCTCTGG15310.11850689058354No Hit
ACATGGGACAGCAGCCTGAGTGCCTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGG15100.11688138783876251No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC14760.11424962149007514No Hit
GGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACCCTAACAGTGGTGGCACAAACTATG14660.11347557256399063No Hit
GGCCAGAAGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGGAATAATTTTGTATCCTGGTACCAGCAG14560.1127015236379061No Hit
GTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACGTGCCAGACTTGGAGCC14560.1127015236379061No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14310.11076640132269482No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG14260.11037937685965254No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG14150.10952792304095957No Hit
CATCTGTCCTCTAGAGAATCCCCTGAGAGCTCCGTTCCTCACCATGGACT14000.10836684965183278No Hit
GTATCAACGCAGAGTACGGGGAGCATCACCCAGCAACCACATCTGTCCTCTAGAGAATCCCCTGAGAGCTCCGTT13990.10828944475922435No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG13910.10767020561835673No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA13880.10743799094053137No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTC13830.1070509664774891No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA13730.10627691755140459No Hit
CCATGTAGGCTGTGCTGATGGACGTGTCCCTGGTCATGGTGACCCAGCCCTGAAACTTCTGTGCATAGTTTGTGC13720.10619951265879612No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13590.10519324905488626No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA13470.10426439034358483No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13440.10403217566575948No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13390.10364515120271722No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG13290.10287110227663271No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT13270.1027162924914158No Hit
CCCAGCAACCACATCTGTCCTCTAGAGAATCCCCTGAGAGCTCCGTTCCTCACCATGGACTGGACCTGGAGGATC13020.10078117017620448No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13000.1006263603909876No Hit
CCTCTAGAGAATCCCCTGAGAGCTCCGTTCCTCACCATGGACTGGACCTG12950.10023933592794533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2900.047.6264421
TGGTATC3050.045.2771452
GGTATCA7850.041.3470341
CTTATAC1350.040.9234661
GTATCAA19150.040.7498321
TATCAAC22000.035.3084562
TCAACGC22700.034.0675664
ATCAACG22600.034.0655443
CAACGCA22750.033.9926875
AACGCAG23400.033.3435256
TTATACA1800.032.6058352
AGAGTAC24900.030.91653311
CGCAGAG25100.030.8053388
CGTATGC1600.030.31748641
CAGAGTA25650.030.01254310
GCAGAGT25950.029.6644299
ATGCCGT1700.028.56184444
ACGGGTA756.138415E-527.61692216
ACGAGAC2000.027.61692222
CCGTTAA500.007712167727.61692216
Produced by FastQC (version 0.11.5)