FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1291908
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCGTCAGATCTCAGCCTCCTCAGCTCCATGTAGGCTGTGCTGATGGACGTGTCCCTGGT47060.3642674246153751No Hit
CTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACCCTAACAGTGGTGG40390.3126383612455376No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT34950.27053009966653974No Hit
GTGTATATAGTAGCCGGTGAAGGTGTATCCAGAAGCCTTACAGGAGACCTTCACTGAGGCCCCAGGCTTCTTCAC32530.2517981156552943No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32120.24862451505834782No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT30770.23817485455620677No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGTAAGGCTTCTGGATACACCTTCACCGG28200.21828179715583465No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT27230.2107735225728148No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC26990.2089158051502119No Hit
GTGTATTACTGTGCGAGAGTTCGTATGGTTCGGGGAGTTACATTCCATTA26180.2026460088489273No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAGGCACTCAGGCTGCTGTCCCATGTT23930.18522990801202563No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC23680.18329478569681432No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG23630.18290776123377206No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGCCAGAAGGTCACCATCTCCTG23430.18135966338160303No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG23080.1786504921403072No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23060.17849568235509028No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22390.17330955455032404No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGCCAGAAGGTCAC22330.1728451251946733No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAGGCACTCAGG21670.16773640228251546No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA21540.16673013867860562No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21440.16595608975252107No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT21430.16587868485991264No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC21120.1634791331890506No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20630.15968629345123647No Hit
GCCTACATGGAGCTGAGGAGGCTGAGATCTGACGACACGGCCGTGTATTA20460.15837041027689278No Hit
CTGTTAGGGTTGATCCATCCCATCCACTCAAGCCCTTGTCCAGGGGCCTG20450.15829300538428434No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT19680.15233282865343353No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG19640.1520232090829997No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT19050.14745632041910106No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC19020.14722410574127567No Hit
ACTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG18720.14490195896302213No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18050.13971583115825584No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17570.13600039631305016No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA17550.13584558652783324No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT17250.1335234397495797No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT17210.13321382017914588No Hit
GGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACCCTAACAGTGGTGGCACAAACTATG17070.13213015168262757No Hit
CCCTAACAGTGGTGGCACAAACTATGCACAGAAGTTTCAGGGCTGGGTCACCATGACCAGGGACACGTCCATCAG16950.13120129297132613No Hit
GTCCTCTAGAGAATCCCCTGAGAGCTCCGTTCCTCACCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGGCAG16850.1304272440452416No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC16750.1296531951191571No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16660.12895655108568102No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16620.12864693151524723No Hit
CAGTAATACACGGCCGTGTCGTCAGATCTCAGCCTCCTCAGCTCCATGTA16580.1283373119448134No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA16330.12640218962960212No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG16110.12469928199221615No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT16030.12408004285134856No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT15960.1235382086030894No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA15900.12307377924743866No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA15800.12229973032135415No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA15680.12137087161005274No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14910.11541069487920191No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACCCTAACAGTGGT14910.11541069487920191No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14730.1140174068122498No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC14510.11231449917486384No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14450.11185006981921314No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC14250.11030197196704408No Hit
ACATGGGACAGCAGCCTGAGTGCCTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGG14140.1094505181483511No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14140.1094505181483511No Hit
CCCAGGAACAGCCCCCAAACTCCTCATCTATGAAAATAATGAGCGACCCTCAGGGATTCCTGACCGATTCTCTGG14110.10921830347052576No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA14010.10844425454444125No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14000.10836684965183278No Hit
GTATCAACGCAGAGTACGGGGAGCATCACCCAGCAACCACATCTGTCCTCTAGAGAATCCCCTGAGAGCTCCGTT13900.10759280072574827No Hit
CCATGTAGGCTGTGCTGATGGACGTGTCCCTGGTCATGGTGACCCAGCCCTGAAACTTCTGTGCATAGTTTGTGC13840.10712837137009756No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGA13660.10573508330314542No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13620.10542546373271161No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG13560.1049610343770609No Hit
GTGCTGATGGACGTGTCCCTGGTCATGGTGACCCAGCCCTGAAACTTCTG13390.10364515120271722No Hit
GGCCAGAAGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGGAATAATTTTGTATCCTGGTACCAGCAG13290.10287110227663271No Hit
CATCTGTCCTCTAGAGAATCCCCTGAGAGCTCCGTTCCTCACCATGGACT13040.10093597996142141No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG13030.10085857506881295No Hit
GTCCCATGTTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGG13020.10078117017620448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18400.042.4947431
GGTATCA7100.038.983081
GTGGTAT2850.038.8463021
GGTATAC459.9985824E-538.3448873
TATCAAC21000.037.0213052
ATCAACG22000.035.451593
TCAACGC22000.035.2769664
CAACGCA22400.034.798315
AACGCAG23250.033.524826
TGGTATC3100.033.43862
CGCAGAG23750.032.819038
AGAGTAC23700.032.7427511
CCGCTAT800.003696272432.0988270
CAGAGTA24650.031.6207710
GCAGAGT24950.031.1023319
ACGCAGA27050.028.9427347
AGTACGG24700.027.92633813
GAGTACG25050.027.67383212
TACGGGG13950.025.96048715
GGGCAGT5400.025.6277681
Produced by FastQC (version 0.11.5)