FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences726280
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7850.10808503607424133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9700.041.521551
GTATCAA23300.040.6290051
TATCAAC26200.035.9632842
TCAACGC27750.034.1599854
ATCAACG27800.033.8631133
CAACGCA28150.033.5502625
AACGCAG28600.033.0223736
AACGTTG750.003486847532.48026770
GTGGTAT3950.032.2451251
ACGCAGA30750.030.825187
CGCAGAG30950.030.6259848
AGAGTAC32450.029.42196811
CAGAGTA32600.029.18124610
GCGGCAT1703.6003985E-728.6590670
GCAGAGT33500.028.4997889
TGGTATC3900.027.3342532
AGTACGG25600.026.15991413
GAGTACG26150.025.74103512
GTTAACG851.2882409E-424.76227456
TACGGGT6400.024.68762415
Produced by FastQC (version 0.11.5)