FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences780921
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGATGCCC52260.6692098176384039No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT47340.6062072860122855No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37380.4786655756472165No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGATGCCCCCGGGCAGAGGGTCAC37280.4773850363865231No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACAACACCATTCACGATGTCATCCCATG36540.46790904585739146No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA36190.4634271584449644No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC34660.44383490775635437No Hit
GATTATTACTGTGCAGCATGGGATGACATCGTGAATGGTGTTGTTTTCGG34370.4401213439003433No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACAACACCATTCACG33860.43359059367080666No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG30920.39594273940641883No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29540.37827129760884903No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27780.3557338066206441No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27400.3508677574300089No Hit
GGGATACTCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG27060.3465139239436511No Hit
GAGTATCCCTGTAGATGAGAAGTTTGGGGGCCGCTCCCGGGAGATGCTGG25090.3212873005079899No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG25050.32077508480371253No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25000.3201348151733658No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC24830.31795789843018696No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24360.31193936390492766No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG23390.29951813307620107No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC22190.2841516619478795No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21980.2814625295004232No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC21510.2754439949751639No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG20920.2678888133370725No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20760.26583995051996295No Hit
GGATGACATCGTGAATGGTGTTGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC19340.24765629301811581No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19010.24343051345782737No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18900.24202192027106456No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18320.23459479255904248No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18120.23203371403765555No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17800.22793598840343648No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG17750.2272957187730897No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG17600.22537490988204953No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17570.2249907481038415No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17390.22268577743459325No Hit
GCATGGGATGACATCGTGAATGGTGTTGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG16990.2175636203918194No Hit
CCCCTGAGGGCCTCTGAGTATCCCTGTAGATGAGAAGTTTGGGGGCCGCTCCCGGGAGATGCTGGTACCAATACA16890.21628308113112593No Hit
GTAATAATCACCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG16380.20975233090158926No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA16150.20680709060199431No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15800.2023252031895672No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGATGCCCCCGGGCAGAGGGTCACCATTTCTTGTTCTGGAGGCAG15220.1948980754775451No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15080.19310532051257426No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGATGCCCCCGGGCAGAGGGTCAC15060.19284921266043556No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14950.19144061947367277No Hit
CCCTCAGCGTCTGATGCCCCCGGGCAGAGGGTCACCATTTCTTGTTCTGG14780.18926370273049387No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT14750.18887954095228582No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT14740.18875148702621647No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGATGCCCCCGGGCAGAGG14700.1882392713219391No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC14550.1863184624308989No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14480.18542208494841347No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGGTGATTATTACTGTGCAG14480.18542208494841347No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG14460.1851659770962748No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG14440.1849098692441361No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACAACACCATTCACGA14350.18375738390951196No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14170.18145241324026373No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14000.17927549649708485No Hit
GTGTTGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC13840.1772266336799753No Hit
ACTCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGG13830.17709857975390597No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT13710.1755619326410738No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13270.16992755989402256No Hit
GGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT13140.16826285885512107No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13130.16813480492905172No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13060.1672384274465663No Hit
GTGCAGCATGGGATGACATCGTGAATGGTGTTGTTTTCGGCGGAGGGACC12880.16493345677731805No Hit
GTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCT12540.1605796232909603No Hit
GGGTCACCATTTCTTGTTCTGGAGGCAGCTCCAACATCGAAACTCAATTT12540.1605796232909603No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCTCTG12500.1600674075866829No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAG12490.15993935366061357No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACAACACCATTCA12370.15840270654778138No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACAACAC12310.1576343829913653No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC12270.15712216728708794No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12270.15712216728708794No Hit
ATGTCATCCCATGCTGCACAGTAATAATCACCCTCATCCTCGGACCGGAG12260.1569941133610186No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC12240.1567380055088799No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12230.15660995158281055No Hit
GGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCT12220.15648189765674123No Hit
GGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11810.15123168668789802No Hit
CTCCCGGGAGATGCTGGTACCAATACACAAATTGAGTTTCGATGTTGGAGCTGCCTCCAGAACAAGAAATGGTGA11780.15084752490968995No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11710.14995114742720456No Hit
CTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTC11590.1484145003143724No Hit
CTCCCGGGAGCGGCCCCCAAACTTCTCATCTACAGGGATACTCAGAGGCCCTCAGGGGTCCCTGACCGATTCT11480.14700590712760958No Hit
GGCAGAGGGTCACCATTTCTTGTTCTGGAGGCAGCTCCAACATCGAAACTCAATTTGTGTATTGGTACCAGCAT11310.1448289903844307No Hit
CTACAGGGATACTCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC11230.1438045589758759No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGATG11160.1429081814933905No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGATGCCCCCGGGC11080.14188375008483572No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC10920.1398348872677262No Hit
GGTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG10860.1390665637113101No Hit
GTTCTGGAGGCAGCTCCAACATCGAAACTCAATTTGTGTATTGGTACCAGCATCTCCCGGGAGCGGCCCCCAAA10850.13893850978524075No Hit
GTTTGGGGGCCGCTCCCGGGAGATGCTGGTACCAATACACAAATTGAGTT10800.13829824015489403No Hit
GTCCGAGGATGAGGGTGATTATTACTGTGCAGCATGGGATGACATCGTGAATGGTGTTGTTTTCGGCGGAGGGAC10750.13765797052454729No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGG10600.1357371616335071No Hit
GTCAGGGACCCCTGAGGGCCTCTGAGTATCCCTGTAGATGAGAAGTTTGGGGGCCGCTCCCGGGAGATGCTGGTA10500.13445662237281364No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG10450.13381635274246692No Hit
GCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACT10400.13317608311212017No Hit
GTGTATTGGTACCAGCATCTCCCGGGAGCGGCCCCCAAACTTCTCATCTA10360.13266386740784278No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10250.13125527422108No Hit
GCTTGGTCCCTCCGCCGAAAACAACACCATTCACGATGTCATCCCATGCTGCACAGTAATAATCACCCTCATCCT10240.13112722029501064No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT10130.12971862710824783No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC10100.12933446533003978No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAAC9970.12766976429113827No Hit
GGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGG9940.12728560251293025No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC9890.1266453328825835No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA9850.12613311717830614No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC9630.12331593080478051No Hit
CCATTTCTTGTTCTGGAGGCAGCTCCAACATCGAAACTCAATTTGTGTATTGGTACCAGCATCTCCCGGGAGCGG9520.12190733761801771No Hit
TTACTGTGCAGCATGGGATGACATCGTGAATGGTGTTGTTTTCGGCGGAG9370.1199865287269775No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT9270.11870598946628404No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC9180.11755350413165994No Hit
GCCCCCAAACTTCTCATCTACAGGGATACTCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT9120.11678518057524384No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9090.11640101879703581No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC9070.11614491094489711No Hit
CAGCCACCCTCAGCGTCTGATGCCCCCGGGCAGAGGGTCACCATTTCTTG9060.11601685701882777No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG8940.11448020990599561No Hit
GTACCAGCATCTCCCGGGAGCGGCCCCCAAACTTCTCATCTACAGGGATACTCAGAGGCCCTCAGGGGTCCCTGA8900.11396799420171824No Hit
TTATTACTGTGCAGCATGGGATGACATCGTGAATGGTGTTGTTTTCGGCG8880.11371188634957954No Hit
CCTCTGAGTATCCCTGTAGATGAGAAGTTTGGGGGCCGCTCCCGGGAGAT8840.11319967064530215No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA8800.11268745494102476No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG8790.11255940101495542No Hit
GGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC8700.1114069156803313No Hit
CTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC8620.11038248427177653No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGG8540.10935805286322177No Hit
AAACAACACCATTCACGATGTCATCCCATGCTGCACAGTAATAATCACCC8500.10884583715894437No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGG8440.1080775136025283No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA8380.10730919004611221No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC8320.10654086648969614No Hit
ATCCCATGCTGCACAGTAATAATCACCCTCATCCTCGGACCGGAGCCCAC8280.10602865078541876No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGG8160.1044920036725866No Hit
CTCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGC8130.10410784189437856No Hit
TCAGTGGGCTCCGGTCCGAGGATGAGGGTGATTATTACTGTGCAGCATGG8050.1030834104858238No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8050.1030834104858238No Hit
CCCCCGGGCAGAGGGTCACCATTTCTTGTTCTGGAGGCAGCTCCAACATC8030.1028273026336851No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC8030.1028273026336851No Hit
GCTCCAACATCGAAACTCAATTTGTGTATTGGTACCAGCATCTCCCGGGA7990.1023150869294077No Hit
CATCTACAGGGATACTCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC7950.10180287122513033No Hit
CCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAG7930.10154676337299164No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT7920.1014187094469223No Hit
CTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCA7890.10103454766871424No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7850.10052233196443687No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)