FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325991
Sequences flagged as poor quality0
Sequence length21-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAACACCCAAGAATAAGTA11140.3417272255982527No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11010.3377393854431564No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAACACCCA10840.3325245175480305No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG9820.30123531017727484No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT9810.3009285532422674No Hit
GTGTATTACTGTGCGAGACAAGAGAGAAAGTGGGGCTCTTACTGGGGCCA8180.25092717283605986No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC8120.24908663122601543No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA8010.24571230494093393No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC7950.2438717633308895No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG7910.24264473559085986No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGTTGACACAGCCACCCTCCGTGTCAGTTTCCCCAGGACAGACGG7480.22945418738554132No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC7380.22638661803546725No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA7370.2260798611004598No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC6910.21196904209011908No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG6760.20736768806500794No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT6640.20368660484491904No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT6630.20337984790991162No Hit
GGCTCTGAGGCCTCCTATGAGTTGACACAGCCACCCTCCGTGTCAGTTTCCCCAGGACAGACGGCCAGGATCAC6520.20000552162483015No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAC5990.1837474040694375No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT5950.18252037632940787No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG5780.17730550843428194No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC5780.17730550843428194No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG5710.17515820988923006No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT5690.17454469601921524No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATTGGTTT5680.17423793908420784No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC5660.17362442521419302No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG5640.1730109113441782No Hit
GACTATTACTGTCAATCAACAGACAGCAGTGATACTTATTCTTGGGTGTTTGGCGGAGGGACCAAGCTGACCGTC5310.16288793248893374No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC5240.16074063394388188No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG5210.15982036313885967No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAACACCCAAGAATAAGTAT5050.15491225217874113No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT5050.15491225217874113No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT4990.15307171056869667No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG4970.15245819669868185No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA4810.14755008573856335No Hit
GTCTTTATATATCACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTACC4680.14356224558346703No Hit
CCTATGAGTTGACACAGCCACCCTCCGTGTCAGTTTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATG4660.1429487317134522No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG4630.14202846090843No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT4610.14141494703841517No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC4560.13988116236337814No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA4520.1386541346233485No Hit
CACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATT4510.1383473776883411No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA4500.1380406207533337No Hit
GGCCAGGCCCCTGTGCTGGTGATATATAAAGACACTGAGAGGCCCTCAGG4450.13650683607829664No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA4380.13435953753324478No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGTTGACACAGCCACCCTCCGTGT4370.13405278059823736No Hit
CAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGT4370.13405278059823736No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC4360.13374602366322996No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC4320.13251899592320032No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG4320.13251899592320032No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT4280.1312919681831707No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGTTGACACAGCCA4240.13006494044314107No Hit
GAGTTGACACAGCCACCCTCCGTGTCAGTTTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTG4240.13006494044314107No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA4200.12883791270311146No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT4140.12699737109306697No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG4140.12699737109306697No Hit
GTGCTGGTGATATATAAAGACACTGAGAGGCCCTCAGGGATCCCTGAGCG4140.12699737109306697No Hit
GTCTCTGGCGGCTCCGTCAGCAGTGGTAGTTCCTATTGGGGCTGGATCCG4110.12607710028804475No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC4060.12454331561300772No Hit
GTATCAACGCAGAGTACGGGGTGGGTCCAGGAGGCAGAACTCTGGGTGTC4020.12331628787297809No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT3920.12024871852290402No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC3820.11718114917282992No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA3780.11595412143280029No Hit
GGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATTGGTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGC3750.11503385062777806No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT3740.11472709369277066No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC3730.11442033675776325No Hit
TCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAACA3690.11319330901773363No Hit
CTATTACTGTCAATCAACAGACAGCAGTGATACTTATTCTTGGGTGTTTG3660.1122730382127114No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT3660.1122730382127114No Hit
GCCACCCTCCGTGTCAGTTTCCCCAGGACAGACGGCCAGGATCACCTGCT3650.11196628127770399No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG3640.11165952434269658No Hit
GTATTACTGTGCGAGACAAGAGAGAAAGTGGGGCTCTTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGC3610.11073925353767436No Hit
CCCCTGTGCTGGTGATATATAAAGACACTGAGAGGCCCTCAGGGATCCCT3610.11073925353767436No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGA3560.10920546886263732No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG3550.1088987119276299No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA3530.10828519805761508No Hit
ATATAAAGACACTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCT3520.10797844112260767No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGTTGACACAGCCACCCTCCGTGT3480.10675141338257804No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT3440.10552438564254842No Hit
GCTCTGGAGATGCATTGCCAAACCAATATGCTTATTGGTACCAGCAGAAG3440.10552438564254842No Hit
CACCTGCACTGTCTCTGGCGGCTCCGTCAGCAGTGGTAGTTCCTATTGGG3420.1049108717725336No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC3360.10307033016248915No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG3360.10307033016248915No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT3320.10184330242245952No Hit
GTATCACTGCTGTCTGTTGATTGACAGTAATAGTCAGCCTCGTCTTCTGC3310.10153654548745211No Hit
CCAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTGATATATAAAGACACTGAGAGGCC3310.10153654548745211No Hit
ACCTATTACAATCCGTCCCTCAAGAGTCGAGTCACCATCTCCGTAGACACGTCCAAGAGCCAGTTCTCCCTGAAG3280.1006162746824299No Hit
CTATTGTCCCTGAGCTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGC3260.10000276081241508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC452.9509465E-853.82308241
CTCGTAT452.9653165E-853.79000539
TCCGCGT200.00691861252.55534762
GTTCGAG200.007051630452.30291454
ACTTGCA200.007327812351.797333
TCGTATG552.5356712E-950.29714640
ACGAGAC552.57387E-950.21227622
AGGGGGG504.2396536E-449.8431970
ATGCCGT451.8012088E-646.20511644
TATCTCG451.8378323E-646.0703236
GAGACCG605.5606506E-946.02791624
CACGAGA651.1279553E-842.4873121
GTGGTAT651.1279553E-842.487311
GGTATCA1900.041.7885061
CCGTCTT503.6873862E-641.63589547
GCCTATC503.8103026E-641.4378433
AGACCGG758.62201E-1041.42512525
GTATCAA4500.041.4251251
TTATACA602.8882278E-740.2744262
TATACAC702.1693268E-839.4525033
Produced by FastQC (version 0.11.5)