FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325991
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11840.36320021104877126No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAACACCCAAGAATAAGTA10800.33129748980800083No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAACACCCA9560.2932596298670822No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG9010.2763879984416748No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT8700.26687853345644513No Hit
GTGTATTACTGTGCGAGACAAGAGAGAAAGTGGGGCTCTTACTGGGGCCA7750.23773662463074133No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG7600.23313527060563022No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC7440.22822715964551168No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7320.2245460764254228No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC7260.22270553481537836No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA7260.22270553481537836No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT6990.21442309757017833No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGTTGACACAGCCACCCTCCGTGTCAGTTTCCCCAGGACAGACGG6990.21442309757017833No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG6980.2141163406351709No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC6940.21288931289514126No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA6410.19663119533974865No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC6360.19509741066471162No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6350.1947906537297042No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT6180.18957578583457826No Hit
GGCTCTGAGGCCTCCTATGAGTTGACACAGCCACCCTCCGTGTCAGTTTCCCCAGGACAGACGGCCAGGATCAC6030.18497443180946713No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC5960.18282713326441527No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATTGGTTT5540.16994334199410413No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT5420.16626225877401524No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC5390.165341987968993No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG5330.16350144635894856No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAC5310.16288793248893374No Hit
GACTATTACTGTCAATCAACAGACAGCAGTGATACTTATTCTTGGGTGTTTGGCGGAGGGACCAAGCTGACCGTC5140.1576730645938078No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA5130.15736630765880041No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT5110.1567527937887856No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG5020.1539919813737189No Hit
GTCTTTATATATCACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTACC4980.15276495363368928No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG4920.15092441202364482No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA4800.14724332880355595No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC4780.1466298149335411No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG4730.14509603025850407No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT4710.14448251638848925No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA4660.1429487317134522No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG4550.13957440542837074No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGTTGACACAGCCA4510.1383473776883411No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGTTGACACAGCCACCCTCCGTGT4490.13773386381832628No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG4440.13620007914328922No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAACACCCAAGAATAAGTAT4390.13466629446825218No Hit
GGCCAGGCCCCTGTGCTGGTGATATATAAAGACACTGAGAGGCCCTCAGG4370.13405278059823736No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA4300.13190548205318553No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC4270.13098521124816329No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT4230.12975818350813367No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG4220.12945142657312625No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT4200.12883791270311146No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC4200.12883791270311146No Hit
GTGCTGGTGATATATAAAGACACTGAGAGGCCCTCAGGGATCCCTGAGCG4200.12883791270311146No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC4150.1273041280280744No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA4090.12546358641802993No Hit
CAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGT4080.12515682948302254No Hit
GTCTCTGGCGGCTCCGTCAGCAGTGGTAGTTCCTATTGGGGCTGGATCCG4030.1236230448079855No Hit
CCCCTGTGCTGGTGATATATAAAGACACTGAGAGGCCCTCAGGGATCCCT4020.12331628787297809No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT4010.12300953093797068No Hit
GGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATTGGTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGC3990.12239601706795586No Hit
GTATCAACGCAGAGTACGGGGTGGGTCCAGGAGGCAGAACTCTGGGTGTC3980.12208926013294846No Hit
CCTATGAGTTGACACAGCCACCCTCCGTGTCAGTTTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATG3970.12178250319794105No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT3960.12147574626293364No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA3940.12086223239291882No Hit
CACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATT3930.12055547545791143No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT3910.1199419615878966No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG3890.11932844771788179No Hit
ATATAAAGACACTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCT3860.11840817691285954No Hit
GAGTTGACACAGCCACCCTCCGTGTCAGTTTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTG3840.11779466304284474No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT3790.1162608783678077No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGTTGACACAGCCACCCTCCGTGT3680.11288655208272622No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC3640.11165952434269658No Hit
GCCACCCTCCGTGTCAGTTTCCCCAGGACAGACGGCCAGGATCACCTGCT3590.11012573966765954No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA3580.10981898273265214No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT3580.10981898273265214No Hit
GCTTGGTCCCTCCGCCAAACACCCAAGAATAAGTATCACTGCTGTCTGTTGATTGACAGTAATAGTCAGCCTCGT3570.10951222579764473No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA3470.10644465644757063No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC3420.1049108717725336No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG3420.1049108717725336No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT3400.10429735790251878No Hit
GCTCTGGAGATGCATTGCCAAACCAATATGCTTATTGGTACCAGCAGAAG3400.10429735790251878No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC3370.10337708709749656No Hit
GTATTACTGTGCGAGACAAGAGAGAAAGTGGGGCTCTTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGC3360.10307033016248915No Hit
ACCTATTACAATCCGTCCCTCAAGAGTCGAGTCACCATCTCCGTAGACACGTCCAAGAGCCAGTTCTCCCTGAAG3360.10307033016248915No Hit
GTATCACTGCTGTCTGTTGATTGACAGTAATAGTCAGCCTCGTCTTCTGC3360.10307033016248915No Hit
CTATTACTGTCAATCAACAGACAGCAGTGATACTTATTCTTGGGTGTTTG3320.10184330242245952No Hit
CCAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTGATATATAAAGACACTGAGAGGCC3300.1012297885524447No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT3280.1006162746824299No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCAGT150.00234184769.0903241
CCGAGTT252.391377E-455.98605759
TAAGATT252.469608E-455.62256650
TTTACTT302.71375E-454.56815369
GGTATCA1700.048.7696381
CGAGTTC305.8442604E-446.71319660
GCCGGTT404.69206E-543.58313853
TCCGTTT404.9467082E-543.1947237
CTCCGTT404.9511968E-543.18808436
GTATCAA4500.042.2218671
TAGGCAA600.001087885141.1658470
TTTTACT456.57527E-541.1590668
TTAGGCA604.1476324E-640.92611769
GCCGAGT350.001257879439.977658
TGCCGAG350.001261733439.95274457
TTTGCCG350.001269469439.90312255
GGTAAGA350.001300790339.70585348
GTGGTAT350.001337765839.4801861
TCTAATA350.001342825839.4498832
CTAATAC350.00134586939.431733
Produced by FastQC (version 0.11.5)