FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771338
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCTTCAGACTGGAGGCCACTGATGGCCAGGGAGG59360.7695718349154327No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC49270.6387601803619166No Hit
GATTATTACTGTGCAACATGGGATGACAGCCTGAATGGTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTA42710.5537131581744968No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA40810.52908063650436No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT40150.5205240763452598No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC35990.46659181837274966No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT32950.4271797837005308No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC32190.417326775032476No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC31570.4092887942769577No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG30240.3920460291078619No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC27800.3604126854893704No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC27380.35496760175176123No Hit
GACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCATTCAGG25880.3355208741174427No Hit
GTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC24520.3178891743956605No Hit
ACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCATTCAGGC22860.29636812914701466No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGGTGACCTTGGTC22580.29273807332194185No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA22570.2926084284710464No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG21980.2849593822682145No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21440.27795856031985977No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG21140.27406921479299606No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG21020.27251347658225056No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC21020.27251347658225056No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA18100.23465718012077716No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT17310.22441523690003604No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC17070.22130376047854505No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG16980.22013695682048598No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT16940.21961837741690413No Hit
GTCAGGGACCGCTGAGGGCCGCTGATGATCATCATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGGCGGTA16660.21598832159183132No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG16050.20807998568720845No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC15640.20276454680049472No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGAAATACTGTCAGCTGGTACCGCCAGCTCCCAGGAACGGCCCC15390.1995234255281083No Hit
ATCCCATGTTGCACAGTAATAATCAGCCTCATCTTCAGACTGGAGGCCAC15130.1961526594048264No Hit
GCCTTGGGCTGACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA15060.19524514544855823No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA14930.19355976238691727No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14930.19355976238691727No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14600.1892814823073672No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14540.18850361320199446No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAG14500.18798503379841264No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT14390.1865589404385626No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC14350.18604036103498076No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA14200.18409568827154893No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC14150.18344746401707163No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC14070.18241030520990797No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATGATGATCATCAGCGGCCCTCAGCGGTCCCTGACCGATTCTCTGG13860.17968776334110337No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGAAATACTGTCAG13790.17878024938483517No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG13770.17852095968304427No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG13720.177872735428567No Hit
TCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG13550.17566877296334424No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG13530.1754094832615533No Hit
TCTTCAGACTGGAGGCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTT13430.17411303475259873No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC13360.17320552079633053No Hit
GGCCATCAGTGGCCTCCAGTCTGAAGATGAGGCTGATTATTACTGTGCAA13350.17307587594543508No Hit
ACATGGGATGACAGCCTGAATGGTTATGTCTTCGGAACTGGGACCAAGGT13350.17307587594543508No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC13290.17229800684006233No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGAAATACT13140.17035333407663047No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGCCTCCAGTCTGAAGATG13040.1690568855676759No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAG12960.16801972676051224No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC12850.16659363340066222No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA12830.16633434369887132No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12620.1636118018300667No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT12580.16309322242648489No Hit
ATGTTGCACAGTAATAATCAGCCTCATCTTCAGACTGGAGGCCACTGATG12370.16037068055768028No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG12350.16011139085588938No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT12290.15933352175051665No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12130.15725920413618932No Hit
GCCCTCAGCGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG12010.15570346592544385No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCGCTGAGGGCCGCTG11970.155184886521862No Hit
GTCTGAAGATGAGGCTGATTATTACTGTGCAACATGGGATGACAGCCTGAATGGTTATGTCTTCGGAACTGGGAC11970.155184886521862No Hit
GCCCCCAAACTCCTCATCTATGATGATCATCAGCGGCCCTCAGCGGTCCCTGACCGATTCTCTGGCTCCAAGT11940.15479595196917564No Hit
GTCATCCCATGTTGCACAGTAATAATCAGCCTCATCTTCAGACTGGAGGC11760.1524623446530574No Hit
GAAGAAATACTGTCAGCTGGTACCGCCAGCTCCCAGGAACGGCCCCCAAA11730.15207341010037106No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC11690.1515548306967892No Hit
CATCTATGATGATCATCAGCGGCCCTCAGCGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC11590.15025838218783463No Hit
TCATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGGCGGTACCAGCTGACAGTATTTCTTCCGATGTTGGAG11360.14727655061723913No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG11310.14662832636276185No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11290.14636903666097092No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG11210.1453318778538073No Hit
GTTTGGGGGCCGTTCCTGGGAGCTGGCGGTACCAGCTGACAGTATTTCTT11140.14442436389753907No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11110.1440354293448527No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11060.14338720509037542No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGA10880.14105359777425722No Hit
CTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG10720.13897928015992989No Hit
GTGCAACATGGGATGACAGCCTGAATGGTTATGTCTTCGGAACTGGGACC10660.13820141105455713No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT10600.1374235419491844No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10530.1365160279929162No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC10370.13444171037858887No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10360.13431206552769345No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG10150.13158952365888882No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGG10140.1314598788079934No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC10110.13107094425530702No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG10080.13068200970262064No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG9990.12951520604456151No Hit
GACATAACCATTCAGGCTGTCATCCCATGTTGCACAGTAATAATCAGCCT9920.1286076920882933No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9670.1253665708159069No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA9640.12497763626322053No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9400.12186615984172958No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9330.12095864588546137No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9240.11979184222740225No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG9150.11862503856934314No Hit
CAGCGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC9120.11823610401665675No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA9100.11797681431486585No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA9080.11771752461307494No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9060.11745823491128404No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC9050.11732859006038858No Hit
GTTCCGAAGACATAACCATTCAGGCTGTCATCCCATGTTGCACAGTAATAATCAGCCTCATCTTCAGACTGGAGG8990.11655072095501584No Hit
GGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGAAATACTGTCAGCTGGTACCGCCAG8900.11538391729695671No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC8720.1130503099808385No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC8710.11292066512994305No Hit
GTTCTGGAAGCAGCTCCAACATCGGAAGAAATACTGTCAGCTGGTACCGCCAGCTCCCAGGAACGGCCCCCAAA8560.11097599236651118No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC8530.11058705781382482No Hit
GGGTTGGCCTTGGGCTGACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCATTCAGGCTGTCATCC8520.11045741296292935No Hit
GTATGTGATACTACTACTTCTGCTAATGTATGAAATCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTT8510.11032776811203389No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC8490.11006847841024299No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC8450.10954989900666115No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG8390.1087720299012884No Hit
CCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGC8370.1085127401994975No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG8360.10838309534860205No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC8330.10799416079591566No Hit
GCTGAGGGCCGCTGATGATCATCATAGATGAGGAGTTTGGGGGCCGTTCC8330.10799416079591566No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8290.10747558139233383No Hit
CAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTG8280.10734593654143838No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAG8230.1066977122869611No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC8180.10604948803248382No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCT8090.1048826843744247No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA8070.10462339467263379No Hit
GTTCCTGGGAGCTGGCGGTACCAGCTGACAGTATTTCTTCCGATGTTGGA8060.10449374982173833No Hit
GACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACAT8060.10449374982173833No Hit
CCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATC8010.10384552556726104No Hit
GGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGGT8000.10371588071636559No Hit
CTCCTGTGTAGCCTCTGGATCCACCTTCAGTTCCGATTACATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG7890.10228978735651556No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT7840.10164156310203827No Hit
CCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACA7820.10138227340024736No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC7760.1006044042948746No Hit
ATCACATACTACGCAGACTCTGTGAAGGGCCGATTCAGCATCTCCAGAGACAATGCCAAGAACTCACTGTATCTG7740.1003451145930837No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTAC150.002362197568.9487819
TCCAACG150.002362804768.944312
CCGCTAT300.0032994163.36944270
GTGGTAT1400.056.6511881
TGGTATC1450.054.679972
CAACGTC200.007375972351.7182926
AGTTACT200.007383539351.704889
GATGTGT552.6157068E-950.1380710
GTGTATA506.989103E-848.25788513
TTGATTA451.8709743E-645.9628753
CTTGATT451.8709743E-645.9628752
GTCAGAT551.4782927E-743.8736536
GTATCAA12450.043.7619821
GTGTATC405.0147646E-543.1041681
TATACAC405.024369E-543.090193
ACGAGAC405.024369E-543.0901922
GGTATCA4200.042.6936531
TGTATAA602.9273906E-740.21751414
CGTCAGA602.9273906E-740.2175145
CGCTCCG350.001269225439.91165557
Produced by FastQC (version 0.11.5)