FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771338
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCTTCAGACTGGAGGCCACTGATGGCCAGGGAGG55680.7218625297859045No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC48180.6246288916143117No Hit
GATTATTACTGTGCAACATGGGATGACAGCCTGAATGGTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTA41320.5356925239000282No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA37710.4888907327267683No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT37680.4885017981740819No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC36330.4709997433031952No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC33750.4375513717721673No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT32060.41564139197083505No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG29690.3849155623086118No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC29470.38206337558891174No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC27530.3569122745151931No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC25360.3287793418708789No Hit
GACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCATTCAGG23990.3110179972982013No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG22510.29183055936567365No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA22110.2866447653298554No Hit
ACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCATTCAGGC22060.28599654107537814No Hit
GTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC22040.2857372513735872No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG22030.28560760652269174No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGGTGACCTTGGTC21820.28288506465388713No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20630.26745732739732775No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG20590.2669387479937459No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC19160.24839953431569556No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA17810.2308974794448089No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT17070.22130376047854505No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG16570.21482151793377224No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGAAATACTGTCAGCTGGTACCGCCAGCTCCCAGGAACGGCCCC16060.20820963053810393No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC16000.20743176143273118No Hit
GTCAGGGACCGCTGAGGGCCGCTGATGATCATCATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGGCGGTA15890.20600566807288118No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT15580.201986677695122No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15420.19991236008079466No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC15240.19757875276467646No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG15070.19537479029945368No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15070.19537479029945368No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGAAATACT14340.18591071618408533No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA14330.18578107133318986No Hit
ATCCCATGTTGCACAGTAATAATCAGCCTCATCTTCAGACTGGAGGCCAC14240.18461426767513073No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC14230.1844846228242353No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA14210.18422533312244438No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAG14080.18253995006080342No Hit
GCCTTGGGCTGACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA14010.18163243610453522No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT13940.18072492214826705No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT13770.17852095968304427No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC13600.17631699721782151No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG13550.17566877296334424No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG13340.1729462310945396No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC13300.1724276516909578No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG13250.1717794274364805No Hit
GGCCATCAGTGGCCTCCAGTCTGAAGATGAGGCTGATTATTACTGTGCAA13220.17139049288379413No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGAAATACTGTCAG13200.17113120318200323No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC13170.17074226862931685No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC13160.17061262377842137No Hit
TCTTCAGACTGGAGGCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTT12960.16801972676051224No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGCCTCCAGTCTGAAGATG12780.16568611944439401No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12700.16464896063723036No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATGATGATCATCAGCGGCCCTCAGCGGTCCCTGACCGATTCTCTGG12410.16088925996126213No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCGCTGAGGGCCGCTG12320.159722456303203No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12300.1594631666014121No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG12210.15829636294335298No Hit
TCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG12180.15790742839066663No Hit
GTCTGAAGATGAGGCTGATTATTACTGTGCAACATGGGATGACAGCCTGAATGGTTATGTCTTCGGAACTGGGAC12120.15712955928529387No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12080.15661097988171202No Hit
GTTTGGGGGCCGTTCCTGGGAGCTGGCGGTACCAGCTGACAGTATTTCTT11900.15427737256559382No Hit
ACATGGGATGACAGCCTGAATGGTTATGTCTTCGGAACTGGGACCAAGGT11850.15362914831111651No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA11770.15259198950395286No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC11770.15259198950395286No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC11760.1524623446530574No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11690.1515548306967892No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGG11570.14999909248604373No Hit
ATGTTGCACAGTAATAATCAGCCTCATCTTCAGACTGGAGGCCACTGATG11510.14922122338067098No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT11490.14896193367888008No Hit
GCCCTCAGCGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG11450.14844335427529823No Hit
GCCCCCAAACTCCTCATCTATGATGATCATCAGCGGCCCTCAGCGGTCCCTGACCGATTCTCTGGCTCCAAGT11350.14714690576634368No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAG11240.14572081240649365No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT11110.1440354293448527No Hit
GAAGAAATACTGTCAGCTGGTACCGCCAGCTCCCAGGAACGGCCCCCAAA11040.14312791538858452No Hit
GTCATCCCATGTTGCACAGTAATAATCAGCCTCATCTTCAGACTGGAGGC11010.14273898083589814No Hit
CATCTATGATGATCATCAGCGGCCCTCAGCGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC11000.14260933598500267No Hit
GTGCAACATGGGATGACAGCCTGAATGGTTATGTCTTCGGAACTGGGACC10960.14209075658142084No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG10920.14157217717783904No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC10770.13962750441440716No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG10690.1385903456072435No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGA10420.13508993463306618No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC10090.1308116545535161No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10060.13042272000082972No Hit
TCATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGGCGGTACCAGCTGACAGTATTTCTTCCGATGTTGGAG10030.13003378544814334No Hit
CTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG9870.12795946783381604No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG9750.12640372962307056No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9750.12640372962307056No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG9750.12640372962307056No Hit
GACATAACCATTCAGGCTGTCATCCCATGTTGCACAGTAATAATCAGCCT9730.12614443992127966No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9730.12614443992127966No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG9710.12588515021948873No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG9530.1235515429033705No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA9510.1232922532015796No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG9470.12277367379799775No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9450.12251438409620685No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9410.12199580469262501No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9380.12160687013993865No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC9170.11888432827113407No Hit
GGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGAAATACTGTCAGCTGGTACCGCCAG8940.11590249670053855No Hit
GGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGGT8880.11512462759516581No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG8790.11395782393710668No Hit
GTTCCGAAGACATAACCATTCAGGCTGTCATCCCATGTTGCACAGTAATAATCAGCCTCATCTTCAGACTGGAGG8640.11201315117367483No Hit
GGGTTGGCCTTGGGCTGACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCATTCAGGCTGTCATCC8550.11084634751561573No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC8530.11058705781382482No Hit
CAGCGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC8510.11032776811203389No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8490.11006847841024299No Hit
GTTCTGGAAGCAGCTCCAACATCGGAAGAAATACTGTCAGCTGGTACCGCCAGCTCCCAGGAACGGCCCCCAAA8480.10993883355934753No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC8450.10954989900666115No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC8350.10825345049770659No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA8280.10734593654143838No Hit
GTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA8270.10721629169054292No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC8200.10630877773427473No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC8150.10566055347979746No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA8100.10501232922532015No Hit
ATCACATACTACGCAGACTCTGTGAAGGGCCGATTCAGCATCTCCAGAGACAATGCCAAGAACTCACTGTATCTG8070.10462339467263379No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8030.10410481526905196No Hit
CAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTG7950.1030676564618883No Hit
GGTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGA7940.10293801161099284No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7930.10280836676009739No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7920.10267872190920194No Hit
GCTCCAACATCGGAAGAAATACTGTCAGCTGGTACCGCCAGCTCCCAGGA7900.10241943220741102No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA7760.1006044042948746No Hit
GTATGTGATACTACTACTTCTGCTAATGTATGAAATCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTT7750.10047475944397917No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGTAA300.00301051564.8515970
GTGGTAT1450.054.758171
TGCCGTT200.007088560652.24073452
AGTTTGC200.007132793352.15862350
CCCCTAA200.007195807552.04273645
GTTATAA200.00740061951.6746729
CCGATGC200.00742723651.6277713
ATCCGTT200.00742723651.6277712
TGGTATC1450.049.9186972
GGTATCA4500.047.5628971
GTATCAA13600.045.4363251
CTTACAC503.8884627E-641.326333
TATCAAC15250.040.4571342
GCTTGAC350.001343428239.453091
GGATTAT350.001362863639.3379944
TAAGGCC350.001363297939.3354455
TACGGGC1150.038.91040815
ATCAACG17000.036.059253
TCAACGC17000.035.838074
CAACGCA17250.035.3163875
Produced by FastQC (version 0.11.5)