FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences600887
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22960.38210179284957074No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20330.3383331641390145No Hit
GAGTTACAGTACCCCATTCACTTTCGGCCCTGGGACCAAACTTGATATCAAACGAACTGTGGCTGCACCAT19460.32385456832981907No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19090.31769700459487393No Hit
GTCTACATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA17990.2993907340315234No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15690.2611139864899723No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15200.25295937505720706No Hit
GTCTACAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACCCCATTCACTTTCGGCCCTGGGA15030.250130224151962No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14420.2399785650213767No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13470.2241686040803013No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13240.22034092932614618No Hit
GGTCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA13010.21651325457199108No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12970.21584757200605106No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12880.214349786232686No Hit
CCATAGACCAGGAGTTTAGGGGCTTTTCCTGGTTTCTGCTGATACCAATT12750.21218631789338094No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12200.2030331826117057No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11980.19937192849903562No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11780.19604351566933548No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11450.19055163450033033No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11350.18888742808548029No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10790.17956787216232004No Hit
ACAGTACCCCATTCACTTTCGGCCCTGGGACCAAACTTGATATCAAACGA10650.17723798318152997No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGTAGACTGCTGATGGTGAGAGTGAAATCTGTC10640.17707156254004497No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10140.16875053046579472No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10040.16708632405094467No Hit
GTGCAGCCACAGTTCGTTTGATATCAAGTTTGGTCCCAGGGCCGAAAGTG9560.15909813325966446No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9220.1534398314491743No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCCTTAGGGAGA9130.15194204567580927No Hit
GTCTCCATCCTCCCTGTCTACATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAG9010.14994499797798921No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG9000.1497785773365042No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8670.14428669616749903No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8510.14162396590373896No Hit
GTTGTAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA8150.1356328228102788No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8070.13430145767839877No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7870.13097304484869868No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCCATTCACTTTCGGCCCTGGGACCAAACTTGATATCAAACGAA7720.1284767352264236No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT7700.1281438939434536No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7650.12731179073602858No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACTGGATGCACCATAGA7520.1251483223967235No Hit
GATTTCACTCTCACCATCAGCAGTCTACAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACC7410.12331769534038846No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCAAGTTTGGTCCCAGGGCCGAAAGTGAATGGGGTA7400.12315127469890345No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC7380.12281843341593346No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC7310.12165348892553841No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAGCTATTTAAATTGGTATCAGCAGAAA7230.12032212379365838No Hit
GGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGTAGACTGCTGATGGTGAGAGTGAAAT7090.11799223481286832No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT7060.11749297288841329No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7050.11732655224692828No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7020.11682729032247328No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGTAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA6970.11599518711504825No Hit
GTGTATTACTGTGCGAGCGGGCAGAGGGGTTGTAGTAAGGGTTTATGCAG6850.11399813941722821No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC6760.11250035364386317No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6730.11200109171940814No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6680.11116898851198313No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6670.1110025678704981No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6600.10983762338010307No Hit
CTCCTGGTCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT6490.10800699632376803No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6490.10800699632376803No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT6470.10767415504079803No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC6470.10767415504079803No Hit
GGTTGTAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCC6340.10551068670149295No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA6330.10534426606000795No Hit
CACCATCAGCAGTCTACAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACCCCATTCACTTT6300.10484500413555295No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC6190.10301437707921789No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG6100.10151659130585285No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGC252.5707638E-455.18010335
GGTATAC200.00681513152.76148660
TTTCCGT200.007292745651.86550540
CTAACTA306.1913446E-446.17606442
GTGGTAT1100.043.787321
GTATCAA10500.043.251151
TATCGCG350.001348031239.42423236
CTTATAC1050.039.319221
TATCAAC12500.036.3279382
ATCAACG12850.035.3384633
TCAACGC13000.034.930714
CAACGCA13100.034.926675
TTATACA1201.8189894E-1234.4014552
AACGCAG13700.033.648146
TTGGTAT8450.033.35457670
AGAGTAC13650.033.26180611
CGCAGAG14150.032.3295568
GGGATAC1305.456968E-1231.74990518
GCAGAGT14700.031.3539339
ACTAACT450.004550269330.7634241
Produced by FastQC (version 0.11.5)