FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences600887
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23620.39308555518758104No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18970.3156999568970538No Hit
GAGTTACAGTACCCCATTCACTTTCGGCCCTGGGACCAAACTTGATATCAAACGAACTGTGGCTGCACCAT18400.3062139803324086No Hit
GTCTACATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA17250.28707560656163306No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16640.27692394743104776No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15110.25146158928384205No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13800.22966048524930643No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13430.22350292151436124No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13360.22233797702396627No Hit
GTCTACAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACCCCATTCACTTTCGGCCCTGGGA13210.21984166740169117No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13180.21934240547723613No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13010.21651325457199108No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12890.21451620687417103No Hit
GGTCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA12390.2061951747999208No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11970.1992055078575506No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11640.19371362668854544No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11590.19288152348112042No Hit
CCATAGACCAGGAGTTTAGGGGCTTTTCCTGGTTTCTGCTGATACCAATT11300.18805532487805526No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10970.18256344370905012No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10350.17224536393697984No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10190.16958263367321977No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGTAGACTGCTGATGGTGAGAGTGAAATCTGTC10110.16825126854133973No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10060.16741916533391468No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10060.16741916533391468No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9980.16608780020203465No Hit
ACAGTACCCCATTCACTTTCGGCCCTGGGACCAAACTTGATATCAAACGA9740.16209370480639454No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG9450.1572675062033294No Hit
GTGCAGCCACAGTTCGTTTGATATCAAGTTTGGTCCCAGGGCCGAAAGTG9040.15044425990244423No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8980.14944573605353417No Hit
GTCTCCATCCTCCCTGTCTACATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAG8920.14844721220462417No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCCTTAGGGAGA8810.1466165851482891No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8590.142955331035619No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8520.14179038654522397No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8480.14112470397928395No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8420.14012618013037392No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT8330.13862839435700888No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8110.13496714024433878No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCCATTCACTTTCGGCCCTGGGACCAAACTTGATATCAAACGAA8080.13446787831988377No Hit
GTTGTAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA7900.1314723067731537No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA7640.12714537009454357No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAGCTATTTAAATTGGTATCAGCAGAAA7600.12647968752860353No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT7490.1246490604722685No Hit
GATTTCACTCTCACCATCAGCAGTCTACAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACC7490.1246490604722685No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACTGGATGCACCATAGA7390.12298485405741845No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC7220.12015570315217339No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGTAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA7210.11998928251068836No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7190.11965644122771835No Hit
GGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGTAGACTGCTGATGGTGAGAGTGAAAT7140.11882433802029332No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6880.11449740134168321No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCAAGTTTGGTCCCAGGGCCGAAAGTGAATGGGGTA6880.11449740134168321No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6820.11349887749277318No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6780.11283319492683316No Hit
CTCCTGGTCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT6660.11083614722901311No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT6650.1106697265875281No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6620.11017046466307309No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC6500.10817341696525302No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC6470.10767415504079803No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6370.10600994862594798No Hit
GGTTGTAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCC6360.10584352798446296No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACT6350.10567710734297796No Hit
GTGTATTACTGTGCGAGCGGGCAGAGGGGTTGTAGTAAGGGTTTATGCAG6260.10417932156961292No Hit
GATGTAGACAGGGAGGATGGAGACTGGGTCATCTGGATGTCACATCTGGCACCTCGGAGCCAGAGTAGCAGGAGC6040.10051806745694282No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC6030.10035164681545782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1900.043.5191881
GTATACA350.001374319439.2696534
TAGGACC350.001374319439.2696534
GTATCAA11600.039.204791
TATCAAC12700.035.222452
CGAACTA400.002622506834.42683829
CGTTAAA501.9041091E-434.36380817
GCCGTTA501.9041091E-434.36380815
CCGTTAA501.9041091E-434.36380816
ATCAACG13050.034.246393
CAACGCA13250.034.231285
TCAACGC13300.034.1025924
AACGCAG13850.032.7483376
AGTATAC751.7141938E-632.086243
AGAGTAC14100.031.92400211
GTAGTAT553.2814796E-431.320631
GGGGATA450.004302161331.1179356
TTGGTAT8850.030.9826970
CGCAGAG14750.030.7501358
ACGCAGA15050.030.1371777
Produced by FastQC (version 0.11.5)